Correction to Fully Automated Sample Processing and Analysis Workflow for Low-Input Proteome Profiling

Open-tubular trap columns: towards simple and robust liquid chromatography separations for single-cell proteomics

“…PLOTrap sample loading. Sample loading was performed with a modified WPS-3000TPL autosampler 24 (Thermo Fisher). Samples were dispensed into 384 well plates (Part-No.…”

“…An Ultimate 3000 nanocapillary pump (Thermo Fisher) was operated at a flow rate of 910 nL min −1 . 24 The flow was split such that mobile phase passed through the analytical column at a flow rate of ∼60 nL min −1 , while the remaining flow went to waste. The analytical column was 20 cm long, 30 μm i.d.…”

“…1A). 24,25 The trap column is generally a short segment of capillary containing chromatographic media that is similar to that of the analytical column. For such setups, the trap column receives and preconcentrates the sample from the sample loop while salts and any air bubbles are flushed through.…”