Correction to: Evaluating the therapeutic potential of ADAR1 inhibition for triple-negative breast cancer

Kung, Che-Pei; Cottrell, Kyle A.; Ryu, Sua; Bramel, Emily R.; Kladney, Raleigh D.; Bao, Emily A.; Freeman, Eric C.; Sabloak, Thwisha; Maggi, Leonard B.; Weber, Jason D.

doi:10.1038/s41388-021-01669-w

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“…25 Moreover, adenosine deaminase acting on RNA 1 (ADAR1) and DExH-Box Helicase 9 work together to prevent the recognition of self-dsRNAs by innate immune sensors in breast cancer cells. 29,30 Despite the pathological relevance and therapeutic potential of cellular dsRNAs, the repertoire of dsRBPs remains largely elusive. The current golden standard of systemic identification of RBPs relies on ultraviolet (UV) crosslinking and pull-down with oligo-dT beads.…”

Section: Introductionmentioning

confidence: 99%

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Cellular dsRNA interactome reveals the regulatory map of exogenous RNA sensing

Lim,

Lee,

et al. 2024

Preprint

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SUMMARYRNA-binding proteins (RBPs) provide a critical post-transcriptional regulatory layer in determining RNA fate. Currently, UV crosslinking followed by oligo-dT pull-down is the golden standard in identifying the RBP repertoire, but such method is ineffective in capturing RBPs that recognize double-stranded RNAs (dsRNAs). Here, we utilize anti-dsRNA antibody immunoprecipitation followed by quantitative mass spectrometry to comprehensively identify RBPs bound to cellular dsRNAs without any external stimulus. Notably, our dsRNA interactome contains proteins involved in sensing N6-methyladenosine RNAs and stress granule components. We further perform targeted CRISPR-Cas9 knockout functional screening and discover proteins that can regulate the interferon (IFN) response during exogenous RNA sensing. Interestingly, depleting dsRBPs mostly attenuate the IFN response, and they act as antiviral factors during human β-coronavirus HCoV-OC43 infection. Our dsRNA interactome capture provides an unbiased and comprehensive characterization of putative dsRBPs and will facilitate our understanding of dsRNA sensing in both physiological and pathological contexts.

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Section: Introductionmentioning

confidence: 99%