Correction to: Cyclically stretched ACL fibroblasts emigrating from spheroids adapt their cytoskeleton and ligament-related expression profile

Gögele, Clemens; Hoffmann, Christina; Konrad, Jens; Merkel, Rudolf; Schwarz, Silke; Tohidnezhad, Mersedeh; Hoffmann, Bernd; Schulze‐Tanzil, Gundula

doi:10.1007/s00441-021-03472-1

Cited by 3 publications

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“…To analyze this, we immunolabeled the Ki67 antigen and measured the DNA content. It is possible that the LACL derived cells suffered from overload in this study with 4% stretch, although we had shown previously that the same cells showed a significantly higher proliferation rate under 2D conditions, but also when emigrating from 3D spheroids with 14% stretch [ 49 ]. However, it remains debatable to directly compare 3D with 2D stretch conditions since the cells have to sustain the 3D deformation of their complete environment under 3D conditions.…”

Section: Discussionmentioning

confidence: 99%

Maintenance of Ligament Homeostasis of Spheroid-Colonized Embroidered and Functionalized Scaffolds after 3D Stretch

Gögele

Konrad

Hahn

et al. 2021

IJMS

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Anterior cruciate ligament (ACL) ruptures are usually treated with autograft implantation to prevent knee instability. Tissue engineered ACL reconstruction is becoming promising to circumvent autograft limitations. The aim was to evaluate the influence of cyclic stretch on lapine (L) ACL fibroblasts on embroidered scaffolds with respect to adhesion, DNA and sulphated glycosaminoglycan (sGAG) contents, gene expression of ligament-associated extracellular matrix genes, such as type I collagen, decorin, tenascin C, tenomodulin, gap junctional connexin 43 and the transcription factor Mohawk. Control scaffolds and those functionalized by gas phase fluorination and cross-linked collagen foam were either pre-cultured with a suspension or with spheroids of LACL cells before being subjected to cyclic stretch (4%, 0.11 Hz, 3 days). Stretch increased significantly the scaffold area colonized with cells but impaired sGAGs and decorin gene expression (functionalized scaffolds seeded with cell suspension). Stretching increased tenascin C, connexin 43 and Mohawk but decreased decorin gene expression (control scaffolds seeded with cell suspension). Pre-cultivation of functionalized scaffolds with spheroids might be the more suitable method for maintaining ligamentogenesis in 3D scaffolds compared to using a cell suspension due to a significantly higher sGAG content in response to stretching and type I collagen gene expression in functionalized scaffolds.

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Section: Discussionmentioning

confidence: 99%

Maintenance of Ligament Homeostasis of Spheroid-Colonized Embroidered and Functionalized Scaffolds after 3D Stretch

Gögele

Konrad

Hahn

et al. 2021

IJMS

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The actin cytoskeleton: Morphological changes in pre- and fully developed lung cancer

2022

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Actin, a primary component of the cell cytoskeleton can have multiple isoforms, each of which can have specific properties uniquely suited for their purpose. These monomers are then bound together to form polymeric filaments utilizing adenosine triphosphate hydrolysis as a source of energy. Proteins, such as Arp2/3, VASP, formin, profilin, and cofilin, serve important roles in the polymerization process. These filaments can further be linked to form stress fibers by proteins called actin-binding proteins, such as α-actinin, myosin, fascin, filamin, zyxin, and epsin. These stress fibers are responsible for mechanotransduction, maintaining cell shape, cell motility, and intracellular cargo transport. Cancer metastasis, specifically epithelial mesenchymal transition (EMT), which is one of the key steps of the process, is accompanied by the formation of thick stress fibers through the Rho-associated protein kinase, MAPK/ERK, and Wnt pathways. Recently, with the advent of “field cancerization,” pre-malignant cells have also been demonstrated to possess stress fibers and related cytoskeletal features. Analytical methods ranging from western blot and RNA-sequencing to cryo-EM and fluorescent imaging have been employed to understand the structure and dynamics of actin and related proteins including polymerization/depolymerization. More recent methods involve quantifying properties of the actin cytoskeleton from fluorescent images and utilizing them to study biological processes, such as EMT. These image analysis approaches exploit the fact that filaments have a unique structure (curvilinear) compared to the noise or other artifacts to separate them. Line segments are extracted from these filament images that have assigned lengths and orientations. Coupling such methods with statistical analysis has resulted in development of a new reporter for EMT in lung cancer cells as well as their drug responses.

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Harnessing Thermogel Actuation for Driving Directional Stromal Cell Communication and Migration into Columnar Arrays

Vernengo,

Pylostomou,

Wychowaniec

et al. 2024

Preprint

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During developmental processes, cells frequently condense along a preferred axis, creating columnar arrangements—a pivotal step in shaping elongating tissue structures and facilitating gradual building of tissue complexity. Despite advances in biofabrication technology that has allowed researchers to recreate these axial arrangements in vitro in 3D culture, maintaining these patterns for periods of cultivation beyond 7 days has proven challenging, given cells' tendency to exhibit random migratory patterns. In this study, we introduce EXPECT (EXtrusion Patterned Embedded ConstruCTs), a thermosensitive hydrogel based on poly(N-isopropylacrylamide) designed with specific rheological properties enabling the creation of embedded, macroscopic, cell-laden channels within the hydrogel using 3D printing. EXPECT, coupled with mild temperature changes at regular intervals, suppressed the random migratory tendencies of mesenchymal stromal cells (MSCs), guiding the cells to laterally intercalate and aggregate longitudinally. This resulted in the formation of continuous stacked arrangements of MSCs sustained over 36 days of culture. Additionally, EXPECT led to the elongation of initially spaced MSC spheroids toward each other, culminating in their fusion into narrowed, columnar assemblies. Our study presents a versatile and readily applicable approach for orchestrating and maintaining cell communication and movements along a preferred axis outside the developmental niche. By addressing a key limitation in current in vitro 3D culture systems and inducing cell movements reminiscent of both convergent extension and directed chemotaxis, we present a novel tool for studying various facets of development, disease, and repair.

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Correction to: Cyclically stretched ACL fibroblasts emigrating from spheroids adapt their cytoskeleton and ligament-related expression profile

Abstract: A correction to this paper has been published: https://doi.org/10.1007/s00441-021-03472-1

Cited by 3 publications

References 0 publications

Maintenance of Ligament Homeostasis of Spheroid-Colonized Embroidered and Functionalized Scaffolds after 3D Stretch

Maintenance of Ligament Homeostasis of Spheroid-Colonized Embroidered and Functionalized Scaffolds after 3D Stretch

The actin cytoskeleton: Morphological changes in pre- and fully developed lung cancer

Harnessing Thermogel Actuation for Driving Directional Stromal Cell Communication and Migration into Columnar Arrays

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