Correction: The fecal, oral, and skin microbiota of children with Chagas disease treated with benznidazole

Mapping of host-parasite-microbiome interactions reveals metabolic determinants of tropism and tolerance in Chagas disease

“…Overall, the lack of resolution of microbiota alterations in these sites correlates well with our observation of continued alterations of the fecal microbiota and metabolome through early and persistent experimental T. cruzi infection ( 4 ). In accordance with prior reports ( 4 , 11 ), no significant differences in microbial community richness were observed (fig. S4) between infected and uninfected samples at 12 and 89 days after infection.…”

“…4H) also matches with recent findings that infection decreases many acylcarnitines in chronic CD (23). Likewise, several of our microbiome observations in this C3H/HeJ mouse model of infection with luminescent CL Brener parasites [this study and ( 4)] were corroborated in a study of T. cruzi-infected children (11), including the following: (i) absence of microbial richness changes associated with infection, (ii) infection-associated changes in community composition (beta diversity), and (iii) alterations in family Lachnospiraceae in response to infection.…”

“…Tryptophan and tyrosine, in particular, were affected by infection [tryptophan: decreased in the large intestine overall, Mann-Whitney P = 6.8 × 10 −6 (12 days after infection) and P = 0.0043 (89 days after infection); tyrosine: decreased in the large intestine overall, Mann-Whitney P = 0.015 (acute stage), nonsignificant (89 days after infection)]. We have previously demonstrated that experimental T. cruzi infection alters the fecal microbiome and metabolome ( 4 ), a finding that was recently confirmed in T. cruzi– infected children in Bolivia ( 11 ). We therefore sought to evaluate the spatial impact of T. cruzi infection on the microbiota at each collection site in acute-stage disease (except for the esophagus where insufficient material was available to perform both metabolomic and 16 S analyses) and focusing on the cecum and large intestine in persistent infection, given their role as major sites of CD pathogenesis and the unique metabolomic pattern observed at these sites ( Fig.…”

Microbiome Alterations Driven by Trypanosoma cruzi Infection in Two Disjunctive Murine Models

“…Changes in the intestinal microbiome have previously been associated with T. cruzi infection, even in forms of digestive Chagas disease such as megacolon. Reduced relative abundances of Bacteroides , Akkermansia , and Lactobacillus have been reported ( 5 , 6 , 9 , 10 ). This is consistent with our findings, demonstrating that this parasite could directly affect even other taxa that may be key in the interaction with this protozoan and that may have a direct impact on immunological and metabolic changes in the intestinal environment associated with infection.…”

“…Different studies have identified that the host gut microbiota composition can influence Chagas disease’s physiopathology. Robello et al found in 2019 that the presence of T. cruzi produced alterations in the intestinal microbiota in children, producing an increased fecal abundance of Streptococcus , Blautia , Butyrivibrio , and Roseburia and a lower fecal abundance of Bacteroides ( 5 ). These changes have also been observed in murine models using bioluminescent strains of T. cruzi , highlighting a significant intestinal transit that impacts host gut microbiome.…”