Correction: Shelf-Life Evaluation of Bilayered Human Skin Equivalent, MyDerm™

Seet, Wan Tai; Maarof, Manira; Anuar, Khairoji Khairul; Chua, Kien Hui; Irfan, Abdul Wahab Ahmad; Ng, Min Hwei; Aminuddin, B S; Ruszymah, B H I

doi:10.1371/annotation/44cd1027-1f9e-4843-b013-84ca45ae942f

Cited by 12 publications

(15 citation statements)

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“…Despite testing four different storage media, they showed that viability was reduced to 50% by storage day 14. By storage day 28, viability was <5% across all storage groups (27). Closely related to viability of the skin graft is the colony-forming efficiency of keratinocytes, which was inversely correlated with storage time (27).…”

Section: Epidermal Cellsmentioning

confidence: 97%

Concise Review: Considering Optimal Temperature for Short-Term Storage of Epithelial Cells

et al. 2021

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Transplantation of novel tissue-engineered products using cultured epithelial cells is gaining significant interest. While such treatments can readily be provided at centralized medical centers, delivery to patients at geographically remote locations requires the establishment of suitable storage protocols. One important aspect of storage technology is temperature. This paper reviews storage temperature for above-freezing point storage of human epithelial cells for regenerative medicine purposes. The literature search uncovered publications on epidermal cells, retinal pigment epithelial cells, conjunctival epithelial cells, corneal/limbal epithelial cells, oral keratinocytes, and seminiferous epithelial cells. The following general patterns were noted: (1) Several studies across different cell types inclined toward 4 and 16°C being suitable short-term storage temperatures. Correspondingly, almost all studies investigating 37°C concluded that this storage temperature was suboptimal. (2) Cell death typically escalates rapidly following 7–10 days of storage. (3) The importance of the type of storage medium and its composition was highlighted by some of the studies; however, the relative importance of storage medium vs. storage temperature has not been investigated systematically. Although a direct comparison between the included investigations is not reasonable due to differences in cell types, storage media, and storage duration, this review provides an overview, summarizing the work carried out on each cell type during the past two decades.

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Section: Epidermal Cellsmentioning

confidence: 97%

Concise Review: Considering Optimal Temperature for Short-Term Storage of Epithelial Cells

et al. 2021

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“…Redundant skin tissue samples were obtained from three consenting healthy patients (n = 3) who had undergone abdominoplasty or face-lift surgery. The tissue samples were processed and cultured as described elsewhere [3]. Briefly, the redundant skin was minced, digested with 0.6% type I collagenase (Worthington, Columbia, NJ, USA) for 4-6 h in a 37 • C incubator shaker (Stuart, Staffordshire, UK) and dissociated using 0.05% trypsin-EDTA (Gibco, Gaithersburg, MD, USA) for 8-10 min.…”

Section: Cell Isolation and Culturementioning

confidence: 99%

“…Therefore, these mediators or growth factors have the potential to be used as supplementary therapies in wound treatment. Single-layer keratinocytes, single-layer fibroblasts, and bilayered skin constructs have healing potential [2][3][4] for tissue regeneration. These constructs secrete essential factors such as cytokines, chemokines, and growth factors that are important for wound healing [5].…”

Section: Introductionmentioning

confidence: 99%

Hybrid Collagen Hydrogel/Chondroitin-4-Sulphate Fortified with Dermal Fibroblast Conditioned Medium for Skin Therapeutic Application

et al. 2021

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The current strategy for rapid wound healing treatment involves combining a biomaterial and cell-secreted proteins or biomolecules. This study was aimed at characterizing 3-dimensional (3D) collagen hydrogels fortified with dermal fibroblast-conditioned medium (DFCM) as a readily available acellular skin substitute. Confluent fibroblasts were cultured with serum-free keratinocyte-specific medium (KM1 and KM2) and fibroblast-specific medium (FM) to obtain DFCM. Subsequently, the DFCM was mixed with collagen (Col) hydrogel and chondroitin-4-sulphate (C4S) to fabricate 3D constructs termed Col/C4S/DFCM-KM1, Col/C4S/DFCM-KM2, and Col/C4S/DFCM-FM. The constructs successfully formed soft, semi-solid and translucent hydrogels within 1 h of incubation at 37 °C with strength of <2.5 Newton (N). The Col/C4S/DFCM demonstrated significantly lower turbidity compared to the control groups. The Col/C4S/DFCM also showed a lower percentage of porosity (KM1: 35.15 ± 9.76%; KM2: 6.85 ± 1.60%; FM: 14.14 ± 7.65%) compared to the Col (105.14 ± 11.87%) and Col/C4S (143.44 ± 27.72%) constructs. There were no changes in both swelling and degradation among all constructs. Fourier transform infrared spectrometry showed that all groups consisted of oxygen–hydrogen bonds (O-H) and amide I, II, and III. In conclusion, the Col/C4S/DFCM constructs maintain the characteristics of native collagen and can synergistically deliver essential biomolecules for future use in skin therapeutic applications.

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“…Redundant skin tissue samples from abdominoplasty were obtained from three consenting healthy patients (n = 3) and were processed as described elsewhere [36]. The samples were digested with 0.6% type I collagenase (Worthington, NJ, USA) for 4-6 h, followed by 8-10 min cell dissociation using 0.05% trypsin-EDTA (Gibco, Carlsbad, CA, USA).…”

Section: Cell Isolation and Culturementioning

confidence: 99%

Concentration Dependent Effect of Human Dermal Fibroblast Conditioned Medium (DFCM) from Three Various Origins on Keratinocytes Wound Healing

Maarof

Chowdhury

Saim

et al. 2020

IJMS

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Fibroblasts secrete many essential factors that can be collected from fibroblast culture medium, which is termed dermal fibroblast conditioned medium (DFCM). Fibroblasts isolated from human skin samples were cultured in vitro using the serum-free keratinocyte-specific medium (Epilife (KM1), or define keratinocytes serum-free medium, DKSFM (KM2) and serum-free fibroblast-specific medium (FM) to collect DFCM-KM1, DFCM-KM2, and DFCM-FM, respectively). We characterised and evaluated the effects of 100–1600 µg/mL DFCM on keratinocytes based on attachment, proliferation, migration and gene expression. Supplementation with 200–400 µg/mL keratinocyte-specific DFCM-KM1 and DFCM-KM2 enhanced the attachment, proliferation and migration of sub-confluent keratinocytes, whereas 200–1600 µg/mL DFCM-FM significantly increased the healing rate in the wound healing assay, and 400–800 µg/mL DFCM-FM was suitable to enhance keratinocyte attachment and proliferation. A real-time (RT2) profiler polymerase chain reaction (PCR) array showed that 42 genes in the DFCM groups had similar fold regulation compared to the control group and most of the genes were directly involved in wound healing. In conclusion, in vitro keratinocyte re-epithelialisation is supported by the fibroblast-secreted proteins in 200–400 µg/mL DFCM-KM1 and DFCM-KM2, and 400–800 µg/mL DFCM-FM, which could be useful for treating skin injuries.

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Correction: Shelf-Life Evaluation of Bilayered Human Skin Equivalent, MyDerm™

Cited by 12 publications

References 0 publications

Concise Review: Considering Optimal Temperature for Short-Term Storage of Epithelial Cells

Concise Review: Considering Optimal Temperature for Short-Term Storage of Epithelial Cells

Hybrid Collagen Hydrogel/Chondroitin-4-Sulphate Fortified with Dermal Fibroblast Conditioned Medium for Skin Therapeutic Application

Concentration Dependent Effect of Human Dermal Fibroblast Conditioned Medium (DFCM) from Three Various Origins on Keratinocytes Wound Healing

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