Correction:  Reaction Rate Method for Ammonia Blood Urea Nitrogen Utilizing Pentacyanonitrosyloferrate Catalyzed Berthelot Reaction.

Weichselbaum, Theodore E.; Hagerty, John C.; Mark, Harry B.

doi:10.1021/ac60277a048

Cited by 2 publications

(2 citation statements)

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“…Hematological parameters including hemoglobin (Hb), red blood corpuscles (RBC), erythrocyte sedimentation (ESR), packed cell volume (PCV), total white blood cells (WBC) and platelets were determined according to methods of Docie (1958). Biochemical parameters including serum glutamate oxaloacetate transaminase (SGOT), serum glutamate pyruvate transaminase (SGPT) (Reitman and Frankel, 1957), alkaline phosphatase (ALP) (Kind and King, 1954), gamma glutamyl transpeptidase (GGTP) (Szasz, 1969), total bilirubin (BRN) (Malloy and Evelyn, 1937), total protein (TP) (Lowry et al, 1951), total cholesterol (Varley et al, 1980), glucose (Folin and Wu, 1920), urea, blood urea nitrogen (BUN) (Weichselbaum et al, 1969) and creatinine (Henry, 1974) were analyzed following the standard methods and the electrolytes Na + , K + and Cl − in serum were estimated using flame photometer.…”

Section: Subchronic Toxicity Studies In Ratsmentioning

confidence: 99%

Hepatoprotective and toxicological assessment of an ethnomedicinal plant Euphorbia fusiformis Buch.-Ham.ex D.Don

Anusuya

Raju

Manian

2010

Journal of Ethnopharmacology

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Section: Subchronic Toxicity Studies In Ratsmentioning

confidence: 99%

Hepatoprotective and toxicological assessment of an ethnomedicinal plant Euphorbia fusiformis Buch.-Ham.ex D.Don

Anusuya

Raju

Manian

2010

Journal of Ethnopharmacology

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“…Urease activity was measured by the Berthelot determination (Weichselbaum et al, 1969). Aliquots of 200 L of reaction mixture were removed and acidified with 400 L of 180 mM sulfuric acid, then diluted 1:10 with water and analyzed with a colorimetric method.…”

Section: Enzyme Assaymentioning

confidence: 99%

Isoelectrically trapped enzymatic bioreactors in a multimembrane cell coupled to an electric field: Theoretical modeling and experimental validation with urease

Nembri

Bossi

Ermakov

et al. 1997

Biotechnol. Bioeng.

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A novel type of immobilized enzyme reactor operating under an electric field is here reported: a multicompartment immobilized enzyme reactor (MIER). In this experimental set‐up, the enzyme and zwitterionic buffering ions are trapped in between two isoelectric membranes, having isoelectric point (pl) values so far apart as to trap the enzyme by an isoelectric mechanism, while allowing operation at pH optima, even when the latter pH value is quite removed from the enzyme pl. As an example, urease (pl 4.9) is trapped between a pl 4.0 and a pl 8.0 membranes, thus permitting operation (via suitable amphoteric ions buffering at pH 7.5) at the pH of optimum of activity (pH 7.5). The charged product (ammonium ions) quickly leaves the enzyme chamber under the influence of the electric field, thus allowing sustained activity for much longer time periods than in conventional reactors. As an example, while in a batch reactor 90% of original enzyme activity is lost in 200 min, only 2% activity is lost in the same period in the MIER reactor. As an additional bonus, the MIER reactor allows conversion rates of ∼95% in a wide range of substrate concentrations, whereas batch‐type reactors rarely achieve better than 50% conversion under comparable experimental conditions. © 1997 John Wiley & Sons, Inc.

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Correction: Reaction Rate Method for Ammonia Blood Urea Nitrogen Utilizing Pentacyanonitrosyloferrate Catalyzed Berthelot Reaction.

Cited by 2 publications

References 1 publication

Hepatoprotective and toxicological assessment of an ethnomedicinal plant Euphorbia fusiformis Buch.-Ham.ex D.Don

Hepatoprotective and toxicological assessment of an ethnomedicinal plant Euphorbia fusiformis Buch.-Ham.ex D.Don

Isoelectrically trapped enzymatic bioreactors in a multimembrane cell coupled to an electric field: Theoretical modeling and experimental validation with urease

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