Correction: Qualitative analysis of biosurfactants from Bacillus species exhibiting antifungal activity

Sarwar, Ambrin; Brader, Günter; Corretto, Erika; Aleti, Gajender; Ullah, Muhammad Abaid; Sessitsch, Angela; Hafeez, Fauzia Yusuf

doi:10.1371/journal.pone.0201624

Cited by 11 publications

(4 citation statements)

References 1 publication

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“…The GC-MS analysis of the Cp24 crude biosurfactant revealed the presence of 19 compounds. The nature of the majority of detected compounds was the fatty acids and peptides components of the lipopeptide biosurfactant, which conforms with the previously identified nature of B. amyloliquefaciens biosurfactants as a lipopeptide surfactant (Sarwar et al, 2018;Wu et al, 2022). The complex nature of biosurfactants and their mosaic distribution of polarity, as well as branched or circular structures, give them remarkable physical properties compared to synthetic surfactants.…”

Section: A B Figuresupporting

confidence: 83%

“…Cyclic peptides detected in the Cp24 extract have been detected as products by many Bacillus sp. and have been reported for many potential applications in the agricultural, pharmaceutical, and biotechnology industries due to their dynamic surface properties (Sarwar et al, 2018). Previous studies showed that the diketopiperazines (DKPs) cyclopeptides ((Cyclo(Leu-Pro) and Cyclo (Leu-Leu)), similar to those detected in our extract, inhibit quorum sensing mechanisms and biofilm formation by various microorganisms, such as soft rot-causing pathogen Lelliottia amnigena RCE and P.aeruginosa (Rashiya et al, 2021;Kachhadia et al, 2022).…”

Section: A B Figurementioning

confidence: 52%

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Biosurfactant from Nile Papyrus endophyte with potential antibiofilm activity against global clones of Acinetobacter baumannii

Amer

Wasfi

Hamed

2023

Front. Cell. Infect. Microbiol.

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Acinetobacter baumannii is a leading cause of biofilm-associated infections, particularly catheter-related bloodstream infections (CRBSIs) that are mostly recalcitrant to antimicrobial therapy. One approach to reducing the burden of CRBSIs is inhibiting biofilm formation on catheters. Owing to their prodigious microbial diversity, bacterial endophytes might be a valuable source of biosurfactants, which are known for their great capacity to disperse microbial biofilms. With this in mind, our study aimed to screen bacterial endophytes from plants growing on the banks of the River Nile for the production of powerful biosurfactants capable of reducing the ability of A. baumannii to form biofilms on central venous catheters (CVCs). This was tested on multidrug- and extensive drug-resistant (M/XDR) clinical isolates of A. baumannii that belong to high-risk global clones and on a standard strain of A. baumannii ATCC 19606. The drop collapse and oil dispersion assays were employed in screening the cell-free supernatants (CFS) of all endophytes for biosurfactant activity. Of the 44 bacterial endophytes recovered from 10 plants, the CFS of Bacillus amyloliquefaciens Cp24, isolated from Cyperus papyrus, showed the highest biosurfactant activity. The crude biosurfactant extract of Cp24 showed potent antibacterial activity with minimum inhibitory concentrations (MICs) ranging from 0.78 to 1.56 mg/ml. It also showed significant antibiofilm activity (p-value<0.01). Sub-MICs of the extract could reduce biofilm formation by up to 89.59%, while up to 87.3% of the preformed biofilms were eradicated by the MIC. A significant reduction in biofilm formation on CVCs impregnated with sub-MIC of the extract was demonstrated by CV assay and further confirmed by scanning electron microscopy. This was associated with three log10 reductions in adhered bacteria in the viable count assay. GC-MS analysis of the crude biosurfactant extract revealed the presence of several compounds, such as saturated, unsaturated, and epoxy fatty acids, cyclopeptides, and 3-Benzyl-hexahydro-pyrrolo [1, 2-a] pyrazine-1,4-dione, potentially implicated in the potent biosurfactant and antibiofilm activities. In the present study, we report the isolation of a B. amyloliquefaciens endophyte from the plant C. papyrus that produces a biosurfactant with potent antibiofilm activity against MDR/XDR global clones of A. baumannii. The impregnation of CVCs with the biosurfactant was demonstrated to reduce biofilms and, hence, proposed as a potential strategy for reducing CRBSIs.

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Section: A B Figuresupporting

confidence: 83%

Section: A B Figurementioning

confidence: 52%

Biosurfactant from Nile Papyrus endophyte with potential antibiofilm activity against global clones of Acinetobacter baumannii

Amer

Wasfi

Hamed

2023

Front. Cell. Infect. Microbiol.

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“…Sarwar et al . (,b) in their investigations of microbial biosurfactants from Bacillus species found that LP extracts displayed antifungal activity against Fusarium moniliforme, Fusarium oxysporum, Fusarium solani and Tricoderma atroviride . Additionally, the LP extracts showed haemolytic activity and potential as biocontrol agents against various Fusarium and Trichoderma species.…”

Section: Antimicrobial and Antifungal Properties Of Biosurfactantsmentioning

confidence: 99%

Microbial biosurfactants: current trends and applications in agricultural and biomedical industries

et al. 2019

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Synthetic surfactants are becoming increasingly unpopular in many applications due to previously disregarded effects on biological systems and this has led to a new focus on replacing such products with biosurfactants that are biodegradable and produced from renewal resources. Microbially derived biosurfactants have been investigated in numerous studies in areas including: increasing feed digestibility in an agricultural context, improving seed protection and fertility, plant pathogen control, antimicrobial activity, antibiofilm activity, wound healing and dermatological care, improved oral cavity care, drug delivery systems and anticancer treatments. The development of the potential of biosurfactants has been hindered somewhat by the myriad of approaches taken in their investigations, the focus on pathogens as source species and the costs associated with large‐scale production. Here, we focus on various microbial sources of biosurfactants and the current trends in terms of agricultural and biomedical applications.

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“…For example, Pseudomonas triggers primordium formation in Agaricus bisporus by removing inhibitory C8 compounds produced by the mycelium (Noble et al, 2009). Bacillus can substantially improve the growth and yield of some edible mushrooms by inhibiting the pathogenic fungi Trichoderma harzianum and Fusarium oxysporum (Velázquez-Cedeño et al, 2008;Sarwar et al, 2018). The continuous cropping obstacle of Ganoderma lingzhi may be related to the decline in the relative abundance of beneficial bacteria such as Sphingomonas, Anaeromyxobacter, Bradyrhizobium and Dehalococcoides in the covering soil (Yuan et al, 2021).…”

Section: Figurementioning

confidence: 99%

Dazomet changes microbial communities and improves morel mushroom yield under continuous cropping

Chen

Shao

Zhou³

et al. 2023

Front. Microbiol.

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Morels (Morchella spp.) are highly prized and popular edible mushrooms. The outdoor cultivation of morels in China first developed at the beginning of the 21st century. Several species, such as Morchella sextelata, M. eximia, and M. importuna, have been commercially cultivated in greenhouses. However, the detriments and obstacles associated with continuous cropping have become increasingly serious, reducing yields and even leading to a complete lack of fructification. It has been reported that the obstacles encountered with continuous morel cropping may be related to changes in the soil microbial community. To study the effect of dazomet treatment on the cultivation of morel under continuous cropping, soil was fumigated with dazomet before morel sowing. Alpha diversity and beta diversity analysis results showed that dazomet treatment altered the microbial communities in continuous cropping soil, which decreased the relative abundance of soil-borne fungal pathogens, including Paecilomyces, Trichoderma, Fusarium, Penicillium, and Acremonium, increased the relative abundance of beneficial soil bacteria, including Bacillius and Pseudomonas. In addition, the dazomet treatment significantly increased the relative abundance of morel mycelia in the soil and significantly improved morel yield under continuous cropping. These results verified the relationship between the obstacles associated with continuous cropping in morels and the soil microbial community and elucidated the mechanism by which the obstacle is alleviated when using dazomet treatment.

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Correction: Qualitative analysis of biosurfactants from Bacillus species exhibiting antifungal activity

Cited by 11 publications

References 1 publication

Biosurfactant from Nile Papyrus endophyte with potential antibiofilm activity against global clones of Acinetobacter baumannii

Biosurfactant from Nile Papyrus endophyte with potential antibiofilm activity against global clones of Acinetobacter baumannii

Microbial biosurfactants: current trends and applications in agricultural and biomedical industries

Dazomet changes microbial communities and improves morel mushroom yield under continuous cropping

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