Correction: Proteomic Analysis of C2C12 Myoblast and Myotube Exosome-Like Vesicles: A New Paradigm for Myoblast-Myotube Cross Talk?

Forterre, Alexis V.; Jalabert, Audrey; Berger, Emmanuelle; Baudet, Mathieu; Chikh, Karim; Errazuriz, Elisabeth; Larichaudy, Joffrey De; Chanon, StÃ©phanie; Weiss-Gayet, MichÃ ̈le; Hesse, Anne-Marie; Record, Michel; Géloën, Alain; Lefai, Étienne; Vidal, Hubert; CoutÃ©, Yohann; Rome, Sophie

doi:10.1371/annotation/ecd1e074-2618-4ad0-95c0-efdb467c714b

Cited by 37 publications

(28 citation statements)

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“…More than 96% of the cells were alive after incubation of 24 h. This is in agreement with several studies published earlier using CQDs derived from natural sources [8,9,42]. Considering that the doubling time of C2C12 cells is 15 h [41], an incubation period of 24 h with CQDS was found to be ample for the purpose. Furthermore, the minimal extent of cell death through cyto-toxicological effect, as exhibited by C2C12 cells in the presence of CQD-A, is negligible.…”

Section: Cytotoxicitysupporting

confidence: 91%

“…Since the CQDs were dissolved in water, the corresponding volume of water was added to the control cells. As the doubling time of C2C12 is 15 h [41], the initial incubations were set at 24 h, which is estimated to be ample time for testing the internalization. After 24 h, the wells containing coverslips were fixed using formalin and after washing with PBS, the coverslips were inverted and mounted onto the slides.…”

Section: Internalization Of Cqds By C2c12 Cellsmentioning

confidence: 99%

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Bioimaging of C2C12 Muscle Myoblasts Using Fluorescent Carbon Quantum Dots Synthesized from Bread

Anpalagan

Karakkat

Truskewycz³

et al. 2020

Nanomaterials

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Biocompatible carbon quantum dots (CQDs) have recently attracted increased interest in biomedical imaging owing to their advantageous photoluminescence properties. Numerous precursors of fluorescent CQDs and various fabrication procedures are also reported in the literature. However; the use of concentrated mineral acids and other corrosive chemicals during the fabrication process curtails their biocompatibility and severely limits the utilization of the products in cell bio-imaging. In this study; a facile; fast; and cost-effective synthetic route is employed to fabricate CQDs from a natural organic resource; namely bread; where the use of any toxic chemicals is eliminated. Thus; the novel chemical-free technique facilitated the production of luminescent CQDs that were endowed with low cytotoxicity and; therefore; suitable candidates for bioimaging sensors. The above mentioned amorphous CQDs also exhibited fluorescence over 360–420 nm excitation wavelengths; and with a broad emission range of 360–600 nm. We have also shown that the CQDs were well internalized by muscle myoblasts (C2C12) and differentiated myotubes; the cell lines which have not been reported before.

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Section: Cytotoxicitysupporting

confidence: 91%

Section: Internalization Of Cqds By C2c12 Cellsmentioning

confidence: 99%

Bioimaging of C2C12 Muscle Myoblasts Using Fluorescent Carbon Quantum Dots Synthesized from Bread

Anpalagan

Karakkat

Truskewycz³

et al. 2020

Nanomaterials

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“…We found that MT-derived EVs were enriched in membrane-bound tetraspanins CD63 and CD81, which are common markers for EV subsets released from most cell types (Figure 3A,B). The expression of CD81 is observed on vesicles of various sizes indicating that multivesicular endosomes in muscle cells contain intraluminal vesicles of heterogeneous sizes [27,41]. The expression of proteins associated with EVs, such as tumor susceptibility gene 101 (TSG101), α heat shock 70 kDa protein 4 (HSP70), CD81 and CD63 was further confirmed by Western blot analysis.…”

Section: Mt-derived Ev Characterizationmentioning

confidence: 80%

“…Furthermore, EVs, through their paracrine signaling can be used in tissue engineering to modulate cell recruitment, differentiation, and proliferation [63]. Skeletal muscle cells secrete a large number of myokines and EVs that influence the growth, function and development of muscle tissue [26,27,41]. Transcriptome and proteome studies reported that EVs from C2C12 or human myoblasts are enriched in miRNAs and proteins that are implicated in myogenesis [22,27,40,41,64].…”

Section: Discussionmentioning

confidence: 99%

Extracellular Vesicles from Skeletal Muscle Cells Efficiently Promote Myogenesis in Induced Pluripotent Stem Cells

Baci

Chirivì

Pace

et al. 2020

Cells

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The recent advances, offered by cell therapy in the regenerative medicine field, offer a revolutionary potential for the development of innovative cures to restore compromised physiological functions or organs. Adult myogenic precursors, such as myoblasts or satellite cells, possess a marked regenerative capacity, but the exploitation of this potential still encounters significant challenges in clinical application, due to low rate of proliferation in vitro, as well as a reduced self-renewal capacity. In this scenario, induced pluripotent stem cells (iPSCs) can offer not only an inexhaustible source of cells for regenerative therapeutic approaches, but also a valuable alternative for in vitro modeling of patient-specific diseases. In this study we established a reliable protocol to induce the myogenic differentiation of iPSCs, generated from pericytes and fibroblasts, exploiting skeletal muscle-derived extracellular vesicles (EVs), in combination with chemically defined factors. This genetic integration-free approach generates functional skeletal myotubes maintaining the engraftment ability in vivo. Our results demonstrate evidence that EVs can act as biological “shuttles” to deliver specific bioactive molecules for a successful transgene-free differentiation offering new opportunities for disease modeling and regenerative approaches.

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“…Extracellular vesicles represent a potential source for biomarker discovery and can be used for drug and vaccine delivery conditions [87]. EVs are be considered as integrators of tissue physiology and whole-body homeostasis [88,89] EVs secretion is induced in response to extracellular signals such as ATP, interleukins, depolarization, thrombin receptor activation or by cell stress [90,91] Exosome secretion meanwhile can be induced by stress condition, micronutrient starvation, infection or cancer [92]. Recent studies have shown that skeletal muscle is also able to release EVs into the extracellular space [93,94] and to crosstalk with tissues and organs through this mechanism.…”

Section: Extracellular Vesicle Proteins As a Sub Repertoire Of Tendermentioning

confidence: 99%

Aggregation of Omic Data and Secretome Prediction Enable the Discovery of Candidate Plasma Biomarkers for Beef Tenderness

Boudon

Henry-Berger

Cassar-Malek

2020

IJMS

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Beef quality is a complex phenotype that can be evaluated only after animal slaughtering. Previous research has investigated the potential of genetic markers or muscle-derived proteins to assess beef tenderness. Thus, the use of low-invasive biomarkers in living animals is an issue for the beef sector. We hypothesized that publicly available data may help us discovering candidate plasma biomarkers. Thanks to a review of the literature, we built a corpus of articles on beef tenderness. Following data collection, aggregation, and computational reconstruction of the muscle secretome, the putative plasma proteins were searched by comparison with a bovine plasma proteome atlas and submitted to mining of biological information. Of the 44 publications included in the study, 469 unique gene names were extracted for aggregation. Seventy-one proteins putatively released in the plasma were revealed. Among them 13 proteins were predicted to be secreted in plasma, 44 proteins as hypothetically secreted in plasma, and 14 additional candidate proteins were detected thanks to network analysis. Among these 71 proteins, 24 were included in tenderness quantitative trait loci. The in-silico workflow enabled the discovery of candidate plasma biomarkers for beef tenderness from reconstruction of the secretome, to be examined in the cattle plasma proteome.

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Correction: Proteomic Analysis of C2C12 Myoblast and Myotube Exosome-Like Vesicles: A New Paradigm for Myoblast-Myotube Cross Talk?

Cited by 37 publications

References 0 publications

Bioimaging of C2C12 Muscle Myoblasts Using Fluorescent Carbon Quantum Dots Synthesized from Bread

Bioimaging of C2C12 Muscle Myoblasts Using Fluorescent Carbon Quantum Dots Synthesized from Bread

Extracellular Vesicles from Skeletal Muscle Cells Efficiently Promote Myogenesis in Induced Pluripotent Stem Cells

Aggregation of Omic Data and Secretome Prediction Enable the Discovery of Candidate Plasma Biomarkers for Beef Tenderness

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