Correction: Population Genetic Structure of Apple Scab (Venturia inaequalis (Cooke) G. Winter) in Iran

Elucidating genetic variability and population structure in Venturia inaequalis associated with apple scab diseaseusing SSR markers

“…It is the primary cultivated crop in J&K because though the climate conditions are distinct (sub-temperate to true temperate), they are very suitable for the cultivation of apples[37]. This crop suffers huge losses both quantitatively and qualitatively due to frequent epidemics of scab disease [23, 38], which is caused by V . inaequalis .…”

“…ThePCR was performed in a 0.2-ml tube containing 0.5μM forward and reverse primer, 200 μM eachdNTP, 1 unit kappa Taqpolymerase and 1ul of genomic DNA in a 10xkappa buffer and 5 mM MgCl 2 . The PCR was normalized after repetitive cycles till optimal amplification was achieved and consists of 35 cycles involving initial denaturation step at 94°C for 5 min, followed by 94°C for 30 s, annealing at 53°C for 45 s, extension at 72°C for 1min and final extension at 72°C for 15min[23]. The PCR products were electrophoresed in 1% agarose gel in 0.5 X Tris-Borate-EDTA buffers (89 mMTris-HCl, 89 mM boric acid, 2.5 mMEDTAand pH 8.5) at 110V.…”

“…For diversity and structure analysis of selected fungal samples, 28 published SSR primer pairs were used Table 2 [11, 13]Conditions for PCR were initial denaturation at 94°C for 3 min, followed by 35 cycles of denaturation step at 94°C for 30 s, 45 s of annealing at 50–60°C, 1min of extension at 72°C, and a final extension of 15 min at 72°C which was performed in a 10 μl final volume containing 2 μl of 10X PCR buffer, 3 mMMgCl2, 0.5mMdNTP, 0.5μl of Taq DNA polymerase (kappa), 1μM of each primer and 1μl DNA template [23]. The amplified PCR products were resolved in 2.5% agarose gel at 110 V for 3 h. The bands amplified in different isolates using SSR primers in gel werevisualized using a Gel documentation system (Bio Rad, Gel Doc XR system 170–8170).…”