Correction: PI(4,5)P2 forms dynamic cortical structures and directs actin distribution as well as polarity in <i>Caenorhabditis elegans</i> embryos (doi: 10.1242/dev.164988)

Scholze, Melina J.; Barbieux, Kévin; Simone, Alessandro De; Boumasmoud, Mathilde; Süess, Camille C. N.; Wang, Ruijia; Gönczy, Pierre

doi:10.1242/dev.169144

Cited by 7 publications

(2 citation statements)

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“…Notably, this interaction between polarity proteins and phospholipids is also essential during oriented cell division: The junctional adhesion molecule JAM-A mediates the generation of PI(3,4,5)P3 by PI3K via an Cdc42-dependent pathway thus controlling the planar positioning of the mitotic spindle, which is tethered to the PI(3,4,5)P3-enriched cell poles by dynactin (Tuncay et al, 2015). Similar to protein polarity regulators, phospholipid polarity seems to be conserved throughout evolution and different forms of polarity: In anterior-posterior polarized C. elegans zygotes, PI(4,5)P2 accumulates asymmetrically at the anterior cortex, where it regulates actin assembly and the asymmetric localization of the anterior polarity proteins PAR-3, PAR-6 and PKC3 (aPKC) (Scholze et al, 2018). Conversely, downregulation of PAR-3, but not of the posterior determinant PAR-2, results in disturbed anterior PI(4,5)P2 accumulation.…”

Section: Apical Versus Basolateral Polarity: the Par-complex And Its mentioning

confidence: 99%

Phospholipids of the Plasma Membrane – Regulators or Consequence of Cell Polarity?

Krahn

2020

Front. Cell Dev. Biol.

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Cell polarity is a key feature of many eukaryotic cells, including neurons, epithelia, endothelia and asymmetrically dividing stem cells. Apart from the specific localization of proteins to distinct domains of the plasma membrane, most of these cells exhibit an asymmetric distribution of phospholipids within the plasma membrane too. Notably, research over the last years has revealed that many known conserved regulators of apical-basal polarity in epithelial cells are capable of binding to phospholipids, which in turn regulate the localization and to some extent the function of these proteins. Conversely, phospholipid-modifying enzymes are recruited and controlled by polarity regulators, demonstrating an elaborated balance between asymmetrically localized proteins and phospholipids, which are enriched in certain (micro)domains of the plasma membrane. In this review, we will focus on our current understanding of apical-basal polarity and the implication of phospholipids within the plasma membrane during the cell polarization of epithelia and migrating cells.

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Section: Apical Versus Basolateral Polarity: the Par-complex And Its mentioning

confidence: 99%

Phospholipids of the Plasma Membrane – Regulators or Consequence of Cell Polarity?

Krahn

2020

Front. Cell Dev. Biol.

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“…PI(4,5)P 2 clustering has been associated with many cellular components and functions. Particularly, the possible presence of membrane rafts enriched in cholesterol and sphingomyelin at the plasma membrane of eukaryotic cells, as well as the interaction with the cortical cytoskeleton, have been often suggested to promote PI(4,5)P 2 compartmentalization [11][12][13]19,21,[50][51][52][53][54].…”

Section: Determinants Of Pi-(45)p 2 Clustering In Hela Cellsmentioning

confidence: 99%

Quantitative FRET Microscopy Reveals a Crucial Role of Cytoskeleton in Promoting PI(4,5)P2 Confinement

Sarmento

Borges-Araújo

Pinto

et al. 2021

IJMS

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Phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) is an essential plasma membrane component involved in several cellular functions, including membrane trafficking and cytoskeleton organization. This function multiplicity is partially achieved through a dynamic spatiotemporal organization of PI(4,5)P2 within the membrane. Here, we use a Förster resonance energy transfer (FRET) approach to quantitatively assess the extent of PI(4,5)P2 confinement within the plasma membrane. This methodology relies on the rigorous evaluation of the dependence of absolute FRET efficiencies between pleckstrin homology domains (PHPLCδ) fused with fluorescent proteins and their average fluorescence intensity at the membrane. PI(4,5)P2 is found to be significantly compartmentalized at the plasma membrane of HeLa cells, and these clusters are not cholesterol-dependent, suggesting that membrane rafts are not involved in the formation of these nanodomains. On the other hand, upon inhibition of actin polymerization, compartmentalization of PI(4,5)P2 is almost entirely eliminated, showing that the cytoskeleton network is the critical component responsible for the formation of nanoscale PI(4,5)P2 domains in HeLa cells.

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Where does asymmetry come from? Illustrating principles of polarity and asymmetry establishment in Drosophila neuroblasts

Loyer

Januschke

2020

Current Opinion in Cell Biology

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Correction: PI(4,5)P2 forms dynamic cortical structures and directs actin distribution as well as polarity in Caenorhabditis elegans embryos (doi: 10.1242/dev.164988)

Cited by 7 publications

References 0 publications

Phospholipids of the Plasma Membrane – Regulators or Consequence of Cell Polarity?

Phospholipids of the Plasma Membrane – Regulators or Consequence of Cell Polarity?

Quantitative FRET Microscopy Reveals a Crucial Role of Cytoskeleton in Promoting PI(4,5)P2 Confinement

Where does asymmetry come from? Illustrating principles of polarity and asymmetry establishment in Drosophila neuroblasts

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