Correction of spectra for studying dye aggregates by resonance light scattering

Tikhomirov, A. M.; Shmigol, T. A.; Kozhinova, E. A.; Kyagova, Alla A.; Bezdetnaya, Lina; Potapenko, A. Ya.

doi:10.1134/s0006350909050054

Cited by 4 publications

(6 citation statements)

References 12 publications

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“…RLS is widely used for the sensitive detection of chromophore aggregates, including PDT dyes ( e.g. m‐THPC) (27,28).…”

mentioning

confidence: 99%

“…RLS measurements were performed using a Shimadzu RF‐1501 spectrofluorimeter by simultaneous scanning of PS samples with both the excitation and emission monochromators tuned to the same wavelength. Then the recorded RLS spectra were corrected for internal light filter effect and for the sensitivity of the spectrofluorimeter (27). A 5 μ m aqueous solution of merocyanine 540 (MC540, Sigma) was used as an aggregation positive control (27,29).…”

mentioning

confidence: 99%

“…Then the recorded RLS spectra were corrected for internal light filter effect and for the sensitivity of the spectrofluorimeter (27). A 5 μ m aqueous solution of merocyanine 540 (MC540, Sigma) was used as an aggregation positive control (27,29).…”

mentioning

confidence: 99%

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Fully Protected Glycosylated Zinc (II) Phthalocyanine Shows High Uptake and Photodynamic Cytotoxicity in MCF‐7 Cancer Cells

Kimani

Shmigol

Hammond

et al. 2012

Photochem & Photobiology

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Phthalocyanine photosensitizers are effective in anticancer photodynamic therapy (PDT) but suffer from limited solubility, limited cellular uptake and limited selectivity for cancer cells. To improve these characteristics, we synthesized isopropylidene-protected and partially deprotected tetra β-glycosylated zinc (II) phthalocyanines and compared their uptake and accumulation kinetics, subcellular localization, in vitro photocytotoxicity and reactive oxygen species generation with those of disulfonated aluminum phthalocyanine. In MCF-7 cancer cells, one of the compounds, zinc phthalocyanine {4}, demonstrated 10-fold higher uptake, 5-fold greater PDT-induced cellular reactive oxygen species concentration and 2-fold greater phototoxicity than equimolar (9 μm) disulfonated aluminum phthalocyanine. Thus, isopropylidene-protected β-glycosylation of phthalocyanines provides a simple method of improving the efficacy of PDT.

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“…RLS is widely used for the sensitive detection of chromophore aggregates, including PDT dyes ( e.g. m‐THPC) (27,28).…”

mentioning

confidence: 99%

mentioning

confidence: 99%

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Fully Protected Glycosylated Zinc (II) Phthalocyanine Shows High Uptake and Photodynamic Cytotoxicity in MCF‐7 Cancer Cells

Kimani

Shmigol

Hammond

et al. 2012

Photochem & Photobiology

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“…1 , curve 2 ). This new band appears as a result of the formation of aggregates [ 12 ], which is confirmed by the appearance of a band in the RLS spectrum [ 10 ] ( Fig. 1 , curve 4 ).…”

Section: Resultsmentioning

confidence: 74%

“…The spectral measurements were performed using quartz cells with a thickness of 1 cm. The measured RLS spectra were corrected taking into account the effects of the inner optical filtering effect and the sensitivity of the instrument, in accordance with the procedure described in [ 10 ] by Tikhomirov et al .…”

Section: Methodsmentioning

confidence: 99%

Effect of Sodium Chloride on Aggregation of Merocyanine 540 and Photosensitized Inactivation of Staphylococcus aureus and Pseudomonas aeruginosa

CIe

et al. 2011

Acta Naturae

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Merocyanine 540 (MC540) is used as a photosensitizer for the inactivation of microorganisms. The following is already known about MC540: firstly, MC540 exists in distilled water in both monomeric and dimeric forms, and the addition of salts into a MC540 solution leads to the formation of large aggregates that can be detected by the resonance light scattering technique. Secondly, singlet oxygen can only be photogenerated by MC540 monomers. In the present work, we studied the effect of MC540 in the aggregated state on the rate of photosensitized inactivation ofStaphylococcus aureusandPseudomonas aeruginosa. To this end, bacteria either in MC540-containing distilled water or in a 0.25 M sodium chloride aqueous solution also containing MC540 are irradiated (546 nm). The results show that, in the presence of salt, the aggregation of MC540 greatly increases the efficiency of the MC540-photosensitized inactivation ofP. aeruginosaandS. aureus. In the presence of salt, the rates ofP. aeruginosaandS. aureusinactivation increase by factors of 10 and 30, respectively, in comparison with the rate of inactivation observed in the case of distilled water. Our results suggest that a salt-induced photosensitization mechanism can switch from the singlet oxygen to the free-radical pathway.

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