“…It is commonly accepted that automated registration should be followed by manual verification of the visible tumors to determine if the target position is consistent with the planning target volume (PTV). In cases where the target is outside the PTV, manual adjustments are made to avoid a mismatch (Thilmann et al, 2006;Bissonnette et al, 2009;Higgins et al, 2009;Chen et al, 2011), however, some confusion still exists in the practice of employing manual registration of the planning CT and online volumetric images of lung tumors. First, if the tumor is round or oval and large, the tumors in adjacent slices will also be similar, making manual slice-to-slice matching very difficult.…”