Correction of fertility disorders in patients with cryoglobulinemia

Abstract

IntroductionCryoglobulinemia can be among the causes of sperm bad quality. But conventional examination algorithm for patients with idiopathic oligozoospermia makes no provision for cryoglobulin determination in blood serum.Materials and methodsWe examined 55 patients with idiopathic spermatogenesis disorder. Each patient had cryoglobulin determined in blood serum. For this purpose, optical density of the patient's blood serum before and after its 7–day cooling at a temperature of 4°C was compared. Type of cryoglobulins was established by the method evaluation of serum optic density in different periods of cooling incubation (before and after cooling) using the curves of temperature resistance for comparison. Patients with cryoglobulinemia underwent intracutaneous immunization with autoleukocytes separated from heparinized venous blood.ResultsCryoglobulins were revealed in 16 patients of all 55 examined (29.09%): in six patients’ cryoglobulins of second type; in nine – third type and in one patient – cryoglobulins of first type were detected. In a control group, which consisted of 50 men is blood – donors’ frequency of cryoglobulinemia was 2%.Pathogenetic connection between cryoglobulinemia and disturbances in sperm quality is also supported by the fact that after autoleukocyte immunization in patients who positively responded to cryoglobulinemia treatment (14 persons of 16 or 87.5%) spermogram was found to be improved. Thus, in 12 patient number of spermatozoa. In all patients progressive motility and precentage of normal forms increased with spermatoza concentration.ConclusionsIt is reasonable to include determination of cryoglobulins in blood serum for patients with idiopathic oligo– and zoospermia.

Inhibition of cryoglobulin synthesis as a method of positive influence on spermogram indices (clinical observations)

“…This method was described earlier [2-5, 10, 11]. In our investigations [2,3,4], it was established that inhibition of cryoglobulin synthesis has a positive impact on production of spermatozoa, their motility and morphology. Thus, in most patients with idiopathic oligozoospermia, when autoleukocyte immunization resulted in a considerable decrease in the concentration of cryoglobulins, the amount of spermatozoa increased to 20 mln/ml and more (85.71%).…”

“…Thus, in most patients with idiopathic oligozoospermia, when autoleukocyte immunization resulted in a considerable decrease in the concentration of cryoglobulins, the amount of spermatozoa increased to 20 mln/ml and more (85.71%). Simultaneously, their progressive motility and percentage of normal forms improved [2]. However, despite high efficacy of this method, its implementation into clinical practice occurs slowly and incompletely.…”

“…Peculiar difficulties, according to literature data, occur because in 40% of cases it is impossible to determine the causes of spermatogenesis impairment (idiopathic infertility) [1]. The aim of our earlier described investigation [2,3,4], was a determination of the etiology and pathogen-esis of oligozoospermia (OZ) in patients, who after examination, according to the WHO recommendations, were considered as 'actually healthy people'. In these investigations, it was established that more than the third of patients with idiopathic OZ have an increased level of cold-shock precipitating proteins -cryoglobulins in blood.…”

Original inhibition method of excessive synthesis of pro-inflammatory cytokine of tumour necrosis factor α

“…The investigated method of reduction of pro-inflammatory cytokine activity implies two stages: isolation of leukocytes from the peripheral blood and their intradermal introduction into a patient's body. The mechanism of action of intradermal autoleukocyte immunization was described previously [ 19 – 24 ].…”

“…In our previous investigations on the treatment of autoimmune processes, in particular, systemic vasculitis, efficacy of intradermal autoleukocyte immunization was established, which was regarded as a personal method of the cell therapy. A positive influence on the clinical picture of systemic vasculitis and other autoimmune conditions [ 19 – 24 ] theoretically coincides with the influence on the activity of pro-inflammatory cytokines. It was the cause of the trial on intradermal autoleukocyte immunization as the way of decreasing the high level of TNF-α in blood serum [ 25 ].…”