Correction: Nitrate Reduction to Nitrite, Nitric Oxide and Ammonia by Gut Bacteria under Physiological Conditions

Tiso, Mauro; Schechter, Alan N.

doi:10.1371/journal.pone.0127490

Cited by 55 publications

(13 citation statements)

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“…The conversion of nitrate to nitric oxide involves complex pathways requiring the participation of different bacteria and enzymes ( Tiso and Schechter, 2015 ). Previous researchers reported that turkeys are more sensitive to nitrate toxicity than chickens ( Adams et al., 1966 ).…”

Section: Resultsmentioning

confidence: 99%

“…Previous studies have shown that sodium chlorate ( SC ) has a marked antimicrobial effect against Salmonella in the ceca of chickens and turkeys ( McReynolds et al., 2004 , Moore et al., 2006 ). Sodium nitrate ( SN ) has been reported to have antimicrobial activity and acts to stimulate the innate immune response by increasing the production of nitric oxide ( Ascenzi et al., 2003 , Tiso and Schechter, 2015 ). Hence, the objectives of this research note were to evaluate the effect of dietary administration of SC and SN as a HM prophylaxis in poults.…”

Section: Introductionmentioning

confidence: 99%

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Research Note: Evaluation of dietary administration of sodium chlorate and sodium nitrate for Histomonas meleagridis prophylaxis in turkeys

et al. 2020

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Histomoniasis is currently a re-emerging disease of major significance for many commercial turkey and broiler breeder production companies because of the unavailability of drugs or vaccines. The protozoa Histomonas meleagridis ( HM ) requires the presence of enteric microflora to promote the disease. The objectives of this research note were to evaluate the effect of dietary administration of sodium chlorate ( SC ) and sodium nitrate ( SN ) in vitro and in vivo for HM prophylaxis in poults. A total of 128 day-of-hatch female poults obtained from a commercial hatchery were wing-tagged and randomly assigned into 1 of 4 experimental groups: negative control ( NC ), positive control, dietary inclusion of SC (3,200 ppm) and SN (500 ppm). Poults from groups SC and SN started on their respective diets on day 12. All groups, except the NC, were challenged with 2 × 10 5 HM on day 19 . Controls were fed a basal diet, identical to the treatment diets but not supplemented with SC or SN. Body weight gain ( BWG ) was determined weekly, starting on day 1 until day 28, and postchallenge morbidity and mortality were recorded. On day 28 of age, all surviving poults were lesion scored for hepatic and cecal lesions. Ceca and distal ileum were collected on day 28 for bacterial recovery on selective media for total aerobic, lactic acid bacteria, or gram-negative bacteria. The addition of SC and SN in the in vitro growth of HM greatly reduced the growth of the protozoa after 20 h of incubation when compared with the control nontreated group ( P < 0.05). However, dietary supplementation of SC and SN had no effect against HM in vivo, as was demonstrated by BWG, the severity of lesions in the liver and ceca or bacterial recovery of treated poults when compared with the positive control group.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Research Note: Evaluation of dietary administration of sodium chlorate and sodium nitrate for Histomonas meleagridis prophylaxis in turkeys

et al. 2020

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“…Genes associated with this process were observed in higher abundance in the microbiota of NR calves. A previous study has shown that, in the presence of relatively high physiological nitrate concentrations, two common bacterial species colonizing the human intestine ( E. coli and Lactobacillus plantarum ) generate nitrite and, subsequently, ammonia in an oxygen-dependent fashion 46 . There is still limited information about the importance of this pathway in vivo , and further studies are needed to fully interpret the potential impacts of having a higher abundance of genes associated with this function 46 .…”

Section: Discussionmentioning

confidence: 99%

Impacts of feeding preweaned calves milk containing drug residues on the functional profile of the fecal microbiota

Pereira

Carroll

Lima

et al. 2018

Sci Rep

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Feeding drug residue-containing milk to calves is common worldwide and no information is currently available on the impact on the functional profile of the fecal microbiota. Our objective was to characterize the functional profile of the fecal microbiota of preweaned dairy calves fed raw milk with residual concentrations of antimicrobials commonly found in waste milk from birth to weaning. Calves were assigned to a controlled feeding trial being fed milk with no drug residues or milk with antibiotic residues. Fecal samples collected from each calf once a week starting at birth, prior to the first feeding in the trial, until 6 weeks of age. Antibiotic residues resulted in a significant difference in relative abundance of microbial cell functions, especially with genes linked with stress response, regulation and cell signaling, and nitrogen metabolism. These changes could directly impacts selection and dissemination of virulence and antimicrobial. Our data also identified a strong association between age in weeks and abundance of Resistance to Antibiotics and Toxic Compounds. Findings from this study support the hypothesis that drug residues, even at very low concentrations, impact the gut microbiota of calves and result in changes in the functional profile of microbial populations.

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“…3 ) in the diet to nitrite (NO − 2 ), which interacts with gastric acid, resulting in RNS that act as antimicrobial barriers against ingested enteric pathogens (Sobko et al, 2005;Tiso and Schechter, 2015). RNS can cause damage to cellular components, including the metal centers of proteins, membrane lipids and nucleotide bases, and thereby inhibit respiration and interfere with the DNA replication of pathogens (Fang, 2004).…”

Section: Introductionmentioning

confidence: 99%

A Nitric Oxide-Responsive Transcriptional Regulator NsrR Cooperates With Lrp and CRP to Tightly Control the hmpA Gene in Vibrio vulnificus

Choi

Kim

et al. 2021

Front. Microbiol.

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Nitric oxide (NO) is an important antimicrobial effector produced by the host innate immune system to counteract invading pathogens. To survive and establish a successful infection, a fulminating human pathogen Vibrio vulnificus expresses the hmpA gene encoding an NO dioxygenase in an NO-responsive manner. In this study, we identified an Rrf2-family transcriptional regulator NsrR that is predicted to contain the Fe-S cluster coordinated by three cysteine residues. Transcriptome analysis showed that NsrR controls the expression of multiple genes potentially involved in nitrosative stress responses. Particularly, NsrR acts as a strong repressor of hmpA transcription and relieves the repression of hmpA upon exposure to NO. Notably, nsrR and hmpA are transcribed divergently, and their promoter regions overlap with each other. Molecular biological analyses revealed that NsrR directly binds to this overlapping promoter region, which is alleviated by loss of the Fe-S cluster, leading to the subsequent derepression of hmpA under nitrosative stress. We further found that a leucine-responsive regulatory protein (Lrp) negatively regulates hmpA in an NsrR-dependent manner by directly binding to the promoter region, presumably resulting in a DNA conformation change to support the repression by NsrR. Meanwhile, a cyclic AMP receptor protein (CRP) positively regulates hmpA probably through repression of nsrR and lrp by directly binding to each promoter region in a sequential cascade. Altogether, this collaborative regulation of NsrR along with Lrp and CRP enables an elaborate control of hmpA transcription, contributing to survival under host-derived nitrosative stress and thereby the pathogenesis of V. vulnificus.

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Correction: Nitrate Reduction to Nitrite, Nitric Oxide and Ammonia by Gut Bacteria under Physiological Conditions

Cited by 55 publications

References 1 publication

Research Note: Evaluation of dietary administration of sodium chlorate and sodium nitrate for Histomonas meleagridis prophylaxis in turkeys

Research Note: Evaluation of dietary administration of sodium chlorate and sodium nitrate for Histomonas meleagridis prophylaxis in turkeys

Impacts of feeding preweaned calves milk containing drug residues on the functional profile of the fecal microbiota

A Nitric Oxide-Responsive Transcriptional Regulator NsrR Cooperates With Lrp and CRP to Tightly Control the hmpA Gene in Vibrio vulnificus

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