Correction: Individual Case Analysis of Postmortem Interval Time on Brain Tissue Preservation

Blair, John D.; Wang, Qianqian; Hernandez, Damarys; Siedlak, Sandra L.; Rodgers, M. T.; Achar, Rohan K.; Fahmy, Lara M.; Torres, Sandy; Petersen, Robert B.; Zhu, Xiongwei; Casadesús, Gemma; Lee, Hyoung‐gon

doi:10.1371/journal.pone.0157209

Cited by 11 publications

(1 citation statement)

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“…The cellular pH is important in determining the level of activity of specific subsets of proteases and consequently the level of protein degradation. The integrity of the plasma membrane has also been shown to be compromised after death, thus allowing the diffusion of ions in and out of the cell (Blair et al, 2016, Despina et al, 2003 [4,17] . All these alter the quality and preservation of meat.…”

Section: Introductionmentioning

confidence: 99%

Time-dependent alterations of nucleic acids, intracellular proteins and electrolytes in tissues of post-mortem rats

Ebuehi¹,

Abubakar²

2018

Int. J. Adv. Biochem. Res.

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The study is to determine post-mortem changes in nucleic acids in brain and liver, electrolytes and protein levels in brain and kidney, and to evaluate its relation with post-mortem interval (PMI). Twenty-five male Wistar rats (122.2 ± 8.29g) were sacrificed by cervical dislocation, five rats used for immediate dissection (PMI=0); the remaining 20 rats were kept in their cages under the same environmental conditions in other to allow natural decomposition. Four more groups of five rats each were taken and dissected PMI of 15, 18, 24 and 48 hours. The intracellular levels of DNA and RNA in brain and liver were determined by UV spectrophotometry. The sodium (Na), potassium (K), calcium (Ca), phosphate (PO4 2-), and iron (Fe) concentration, were determined. Brain nucleic acids and proteins showed significant changes at p<0.05 with increasing PMI, while K showed significant changes throughout the 48 hours with very high correlation values (r = -0.9). There was decrease in kidney K, and protein while calcium levels increased significantly. The relationship for rat PMI =-109.409 x K + concentration in mg/g of kidney + 161.05) was obtained. These results suggest that brain intracellular electrolytes, nucleic acids and Kidney K, and proteins may be useful for determining PMI.

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Section: Introductionmentioning

confidence: 99%

Time-dependent alterations of nucleic acids, intracellular proteins and electrolytes in tissues of post-mortem rats

Ebuehi¹,

Abubakar²

2018

Int. J. Adv. Biochem. Res.

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Cell-type Specific Expression Quantitative Trait Loci Associated with Alzheimer Disease in Blood and Brain Tissue

Patel

Farrell

Chung

et al. 2020

Preprint

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Because regulation of gene expression is heritable and context-dependent, we investigated AD-related gene expression patterns in cell-types in blood and brain. Cis-expression quantitative trait locus (eQTL) mapping was performed genome-wide in blood from 5,257 Framingham Heart Study (FHS) participants and in brain donated by 475 Religious Orders Study/Memory & Aging Project (ROSMAP) participants. The association of gene expression with genotypes for all cis SNPs within 1Mb of genes was evaluated using linear regression models for unrelated subjects and linear mixed models for related subjects. Cell type-specific eQTL (ct-eQTL) models included an interaction term for expression of “proxy” genes that discriminate particular cell type. Ct-eQTL analysis identified 11,649 and 2,533 additional significant gene-SNP eQTL pairs in brain and blood, respectively, that were not detected in generic eQTL analysis. Of note, 386 unique target eGenes of significant eQTLs shared between blood and brain were enriched in apoptosis and Wnt signaling pathways. Five of these shared genes are established AD loci. The potential importance and relevance to AD of significant results in myeloid cell-types is supported by the observation that a large portion of GWS ct-eQTLs map within 1Mb of established AD loci and 58% (23/40) of the most significant eGenes in these eQTLs have previously been implicated in AD. This study identified cell-type specific expression patterns for established and potentially novel AD genes, found additional evidence for the role of myeloid cells in AD risk, and discovered potential novel blood and brain AD biomarkers that highlight the importance of cell-type specific analysis.

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Increased Expression of Toll-Like Receptor 4 in Skin of Dogs with Discoid Lupus Erythematous (DLE)

Cerbo

Giusti

Mariotti

et al. 2021

Animals

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Discoid lupus erythematous (DLE) is a common autoimmune skin disorder of dogs where keratinocytes play a pivotal role both in the innate and adaptive immune responses. As for the innate response, pattern recognition receptors (PRR), including Toll-like receptors (TLRs), can activate macrophages and immune tissue cells allowing for transmission and transduction of signals through cytokine and chemokine release to improve host defenses. In particular, TLR4 can also recognize endogenous molecules such as heat shock proteins produced during reactions to tissue damage. The aim of this study was to evaluate the expression of TLR4, a bacterial lipopolysaccharide sensor, in the skin of dogs with DLE and in normal skin to evaluate a possible involvement of this receptor in the disease pathogenesis. Skin samples of affected dogs had a diffuse and intense expression of TLR4 in the epidermis. Also, the inflammatory infiltrates were immunolabelled. The expression was significantly higher in DLE skin compared to normal skin (**** p < 0.0001). In conclusion, dogs with DLE showed an altered expression of TLR4, which might play an important pathogenic role in the ongoing immunopathologic process, thus being considered a valuable therapeutic potential target for DLE.

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Correction: Individual Case Analysis of Postmortem Interval Time on Brain Tissue Preservation

Cited by 11 publications

References 1 publication

Time-dependent alterations of nucleic acids, intracellular proteins and electrolytes in tissues of post-mortem rats

Time-dependent alterations of nucleic acids, intracellular proteins and electrolytes in tissues of post-mortem rats

Cell-type Specific Expression Quantitative Trait Loci Associated with Alzheimer Disease in Blood and Brain Tissue

Increased Expression of Toll-Like Receptor 4 in Skin of Dogs with Discoid Lupus Erythematous (DLE)

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