Correction: Human decellularized bone scaffolds from aged donors show improved osteoinductive capacity compared to young donor bone

Smith, Christopher A.; Board, Tim; Rooney, Paul; Eagle, M. J.; Richardson, Stephen M.; Hoyland, Judith A.

doi:10.1371/journal.pone.0187783

Cited by 19 publications

(15 citation statements)

References 1 publication

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“…In addition to quantifying the remaining cellular material, it is also important to evaluate both the macroscopic change in the ECM structure and the biochemical composition to ensure minimal disruption of the ECM composition. For applications where the whole tissue is decellularized without being further broken down into smaller particles, imaging techniques such as scanning/transmission electron microscopy and microcomputed tomography can be used to compare the structure of ECM both before and after decellularization. For more quantitative analyses, the amount of sulfated glycosaminoglycans (sGAGs) and collagen can be evaluated using dimethylmethylene blue (DMMB) and hydroxyproline assays, respectively.…”

Section: General Methods Of Decellularizationmentioning

confidence: 99%

“…The development of a decellularized bone scaffold has been motivated by the need to improve the biocompatibility of allograft bone and the benefit of preserving the bone's native structure . Xu et al successfully decellularized annulus fibrosus tissue from porcine spine while maintaining its macroscopic structure .…”

Section: Postdecellularization Processing Methodsmentioning

confidence: 99%

“…All three methods did not result in significant loss of collagen . Smith et al investigated the effect of donor age on the resulting osteogenic capacity of the isolated human bone following decellularization . The authors reported that bone from an old donor (≥70 years age) was more porous and less dense than that from a young donor (≤50 years age), but the tissues otherwise had similar composition (e.g.,mineral density, calcium/phosphate ratio).…”

Section: Postdecellularization Processing Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Applications of decellularized extracellular matrix in bone and cartilage tissue engineering

Kim

Majid

Melchiorri

et al. 2018

Bioengineering & Transla Med

229

175

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Regenerative therapies for bone and cartilage injuries are currently unable to replicate the complex microenvironment of native tissue. There are many tissue engineering approaches attempting to address this issue through the use of synthetic materials. Although synthetic materials can be modified to simulate the mechanical and biochemical properties of the cell microenvironment, they do not mimic in full the multitude of interactions that take place within tissue. Decellularized extracellular matrix (dECM) has been established as a biomaterial that preserves a tissue's native environment, promotes cell proliferation, and provides cues for cell differentiation. The potential of dECM as a therapeutic agent is rising, but there are many limitations of dECM restricting its use. This review discusses the recent progress in the utilization of bone and cartilage dECM through applications as scaffolds, particles, and supplementary factors in bone and cartilage tissue engineering.

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Section: General Methods Of Decellularizationmentioning

confidence: 99%

Section: Postdecellularization Processing Methodsmentioning

confidence: 99%

Section: Postdecellularization Processing Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Applications of decellularized extracellular matrix in bone and cartilage tissue engineering

Kim

Majid

Melchiorri

et al. 2018

Bioengineering & Transla Med

229

175

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show abstract

“…According to our knowledge, only two research groups considered the decellularization of human bone. Smith and Collaborators [82,83] developed a method based on wash–centrifuge phases (i.e., dH 2 O preheated to 60 °C under shaking, followed by centrifugation at room temperature) and sonication (sterilant solution at 60 °C and then in 70% v / v ethanol at 21 °C). The resulting material was free from the marrow elements, which may interfere with the graft osteointegration.…”

Section: Bonementioning

confidence: 99%

Tissue-Engineered Grafts from Human Decellularized Extracellular Matrices: A Systematic Review and Future Perspectives

Porzionato

Stocco

Barbon

et al. 2018

IJMS

237

191

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Tissue engineering and regenerative medicine involve many different artificial and biologic materials, frequently integrated in composite scaffolds, which can be repopulated with various cell types. One of the most promising scaffolds is decellularized allogeneic extracellular matrix (ECM) then recellularized by autologous or stem cells, in order to develop fully personalized clinical approaches. Decellularization protocols have to efficiently remove immunogenic cellular materials, maintaining the nonimmunogenic ECM, which is endowed with specific inductive/differentiating actions due to its architecture and bioactive factors. In the present paper, we review the available literature about the development of grafts from decellularized human tissues/organs. Human tissues may be obtained not only from surgery but also from cadavers, suggesting possible development of Human Tissue BioBanks from body donation programs. Many human tissues/organs have been decellularized for tissue engineering purposes, such as cartilage, bone, skeletal muscle, tendons, adipose tissue, heart, vessels, lung, dental pulp, intestine, liver, pancreas, kidney, gonads, uterus, childbirth products, cornea, and peripheral nerves. In vitro recellularizations have been reported with various cell types and procedures (seeding, injection, and perfusion). Conversely, studies about in vivo behaviour are poorly represented. Actually, the future challenge will be the development of human grafts to be implanted fully restored in all their structural/functional aspects.

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“…Nyberg et al () developed 3D printed biocomposites of PCL: demineralized bone matrix (DBM) using an extrusion system and characterized them both in vitro (using adipose derived stem cells) and in vivo (murine calvarial defect) with improved osteogenesis in scaffolds containing DBM over PCL control (Nyberg et al, ). Also, few studies are focusing on the combinatorial effects of demineralization and decellularization strategies on subsequent rheology, gelation, collagen content (Sawkins et al, ), and sample variability in terms of donor age (Smith et al, ). Therefore, bone bioprinting will benefit from the rich source of ECM matrix to help develop biomimetic constructs, but clinical application seems farfetched at this point.…”

Section: D Bioprintingmentioning

confidence: 99%

Advances in three‐dimensional bioprinting of bone: Progress and challenges

Midha

Dalela

Sybil

et al. 2019

J Tissue Eng Regen Med

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Several attempts have been made to engineer a viable three‐dimensional (3D) bone tissue equivalent using conventional tissue engineering strategies, but with limited clinical success. Using 3D bioprinting technology, scientists have developed functional prototypes of clinically relevant and mechanically robust bone with a functional bone marrow. Although the field is in its infancy, it has shown immense potential in the field of bone tissue engineering by re‐establishing the 3D dynamic micro‐environment of the native bone. Inspite of their in vitro success, maintaining the viability and differentiation potential of such cell‐laden constructs overtime, and their subsequent preclinical testing in terms of stability, mechanical loading, immune responses, and osseointegrative potential still needs to be explored. Progress is slow due to several challenges such as but not limited to the choice of ink used for cell encapsulation, optimal cell source, bioprinting method suitable for replicating the heterogeneous tissues and organs, and so on. Here, we summarize the recent advancements in bioprinting of bone, their limitations, challenges, and strategies for future improvisations. The generated knowledge will provide deep insights on our current understanding of the cellular interactions with the hydrogel matrices and help to unravel new methodologies for facilitating precisely regulated stem cell behaviour.

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Correction: Human decellularized bone scaffolds from aged donors show improved osteoinductive capacity compared to young donor bone

Abstract: In the Funding section, the grant numbers are missing. The grant number for the John Charnley Trust is 2435. The grant number for the MRC CIC is MC_PC_14112 v.2.

Cited by 19 publications

References 1 publication

Applications of decellularized extracellular matrix in bone and cartilage tissue engineering

Applications of decellularized extracellular matrix in bone and cartilage tissue engineering

Tissue-Engineered Grafts from Human Decellularized Extracellular Matrices: A Systematic Review and Future Perspectives

Advances in three‐dimensional bioprinting of bone: Progress and challenges

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