Correction: Forced mobilization accelerates pathogenesis: characterization of a preclinical surgical model of osteoarthritis

Appleton, T.; McErlain, David D.; Pitelka, V.; Schwartz, Neil; Bernier, Suzanne M.; Henry, James L.; Holdsworth, David W.; Beier, Frank

doi:10.1186/ar2513

Cited by 70 publications

(135 citation statements)

References 2 publications

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“…In established OA, studies of bone of existing noninvasive (in vivo) analytic methods of subchondral bone changes is low, and small changes are difficult to quantify. (8) Several studies in the rabbit and canine anterior cruciate ligament transaction (ACLT) model of OA (9) showed a decrease of trabecular bone volume (10)(11)(12)(13) and a thinning of the subchondral plate (13)(14)(15) early in the disease process. These changes are in contrast to those found in human end-stage OA.…”

Section: Introductionmentioning

confidence: 99%

Similarities and discrepancies in subchondral bone structure in two differently induced canine models of osteoarthritis

Intema

Sniekers

Weinans

et al. 2010

Journal of Bone and Mineral Research

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In osteoarthritis (OA), cartilage degradation is accompanied by subchondral bone changes. The pathogenesis and physiology of bone changes in OA are still unclear. The changes in subchondral bone architecture and cartilage damage were compared in differently induced experimental models of OA. Experimental OA was induced bilaterally by anterior cruciate ligament transection (ACLT) or by cartilage trauma (Groove model); bilateral sham surgery served as control. Lysylpyridinoline (LP, bone resorption) and C-telopeptide of type II collagen (CTX-II, cartilage breakdown) were measured over time. At 20 weeks after surgery, the subchondral cortical plate and trabecular bone of the tibia were analyzed by micro-computed tomography (mCT) and cartilage degeneration was analyzed histologically and biochemically. In both models, cartilage degeneration and cortical subchondral plate thinning were present. CTX-II levels were elevated over time in both models. Subchondral trabecular bone changes were observed only in the ACLT model, not in the Groove model. Correspondingly, LP levels were elevated over time in the ACLT model and not in the Groove model. Interestingly, the trabecular bone changes in the ACLT model were extended to the metaphyseal area. The early decrease in plate thickness, present in both models, as was cartilage damage, suggests that plate thinning is a phenomenon that is intrinsic to the process of OA independent of the cause/induction of OA. On the other hand, trabecular changes in subchondral and metaphyseal bone are not part of a common pathway of OA development and may be induced biomechanically in the destabilized and less loaded ACLT joint. ß

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Section: Introductionmentioning

confidence: 99%

Similarities and discrepancies in subchondral bone structure in two differently induced canine models of osteoarthritis

Intema

Sniekers

Weinans

et al. 2010

Journal of Bone and Mineral Research

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“…In this work, we report the development and application of a high-performance chemical isotope labeling (CIL) liquid chromatography mass spectrometry (LC-MS) method for in-depth submetabolome analysis of samples collected from an animal model of studying disease progression and treatment. A rat model of surgically induced osteoarthritis (OA)14 is used as an example to illustrate the study design, analytical workflow, metabolomic profiling performance and data analysis method.…”

mentioning

confidence: 99%

Chemical Isotope Labeling LC-MS for Monitoring Disease Progression and Treatment in Animal Models: Plasma Metabolomics Study of Osteoarthritis Rat Model

Chen

Wang

et al. 2017

Sci Rep

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We report a chemical isotope labeling (CIL) liquid chromatography mass spectrometry (LC-MS) method generally applicable for tracking metabolomic changes from samples collected in an animal model for studying disease development and treatment. A rat model of surgically induced osteoarthritis (OA) was used as an example to illustrate the workflow and technical performance. Experimental duplicate analyses of 234 plasma samples were carried out using dansylation labeling LC-MS targeting the amine/phenol submetabolome. These samples composed of 39 groups (6 rats per group) were collected at multiple time points with sham operation, OA control group, and OA rats with treatment, separately, using glucosamine/Celecoxib and three traditional Chinese medicines (Epimedii folium, Chuanxiong Rhizoma and Bushen-Huoxue). In total, 3893 metabolites could be detected and 2923 of them were consistently detected in more than 50% of the runs. This high-coverage submetabolome dataset could be used to track OA progression and treatment. Many differentiating metabolites were found and 11 metabolites including 2-aminoadipic acid, saccharopine and GABA were selected as potential biomarkers of OA progression and OA treatment. This study illustrates that CIL LC-MS is a very useful technique for monitoring incremental metabolomic changes with high coverage and accuracy for studying disease progression and treatment in animal models.

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“…[9][10][11][12] However, evaluation of the actual loading and mobility of the animals demonstrated a major drawback in the study setup. Although initially it was intended to create two groups of animals, one with significant loading of the surgically affected joint and one with minimal loading of the affected joint, this was not unambiguously achieved.…”

Section: Discussionmentioning

confidence: 99%

“…11 Forced mobilization of rats after ACLT and partial medial menisectomy leads to an increase in OA compared to normal mobilization, again indicating that loading is important in the development of OA. 12 More importantly, also in human OA, there is an increasing body of literature suggesting that the mechanical environment of the knee during ambulation has a profound influence on the severity, progression, and treatment outcome for osteoarthritis of the knee. Physically demanding occupation, sports subjecting joints to intense loading, and obesity are associated with osteoarthritis, suggesting that more intense joint loading is associated with onset and progression of joint degeneration.…”

mentioning

confidence: 99%

Does loading influence the severity of cartilage degeneration in the canine Groove‐model of OA?

Vos

Interma

El³

et al. 2009

Journal Orthopaedic Research

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Many animal models are used to study osteoarthritis (OA). In these models the role of joint loading in the development of OA is not fully understood. We studied the effect of loading on the development of OA in the canine Groove-model. In ten female beagle dogs OA was induced in one knee according to the Groove-model. The animals were divided in groups with and without forced-loading. Forced-loading was achieved by fixing the contra-lateral limb to the trunk 3 times a week for 4 hours. After 20 weeks joint tissues of all dogs were evaluated. Subjective evaluation revealed less movement with more loading in the forced-loading-group compared to the group without forced-loading. In both groups induction of OA resulted in macroscopical and microscopical OA changes as well as alterations in cartilage metabolism characteristics for OA. Although differences were small, for some parameters they were statistically significant for the forced-loading-group. There were no differences between the contra-lateral healthy joints of both groups. The present study demonstrates that in the Groove-model intensified loading is not a prerequisite for the development of OA, although it adds to some extent to the severity of the OA. ß

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Correction: Forced mobilization accelerates pathogenesis: characterization of a preclinical surgical model of osteoarthritis

Cited by 70 publications

References 2 publications

Similarities and discrepancies in subchondral bone structure in two differently induced canine models of osteoarthritis

Similarities and discrepancies in subchondral bone structure in two differently induced canine models of osteoarthritis

Chemical Isotope Labeling LC-MS for Monitoring Disease Progression and Treatment in Animal Models: Plasma Metabolomics Study of Osteoarthritis Rat Model

Does loading influence the severity of cartilage degeneration in the canine Groove‐model of OA?

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