Correction for Fukunaga et al., “Activation of Protein Kinase Tel1 through Recognition of Protein-Bound DNA Ends”

Abstract

Page 1961, Fig. 1A and C: In the Rad53 and Tel1 panels, which were generated from the same Western blot and autoradiography images, the splice lines were not properly indicated and images were mixed up. The correct versions are shown below (left, A; right, C). Note that identical images were used for the WT cell lanes. Page 1962, Fig. 2A: An incorrect Western blot image was inadvertently used. The correct version is shown below. Note that the Tel1-HA lanes are identical to the ones used in Fig. 1A and C. Page 1962, Fig. 2C: A Western blot image was inadvertently duplicated and presented. Since we could not locate the original image, a set of data directly relevant to the experiment is shown below. These changes do not affect the interpretation of the data and conclusions of the study.

Dynamic Properties of the DNA Damage Response Mre11/Rad50 Complex

“…Although it is clear that Tel1/ATM activation required its recruitment to damaged DNA and MRN/X complexes [75,76], the necessity of Rad50-mediated ATP hydrolysis [10] and the unwinding of DNA ends [12] for Tel1/ATM stimulation were reported, although the resting state conformation seems to be required for Tel1 activation [19]. Consistently, Rad50 A78T mutation in S. cerevisiae, which destabilizes the resting state of the Mre11/Rad50 tetramer as described above, was also reported to counteract Tel1 activity [28].…”