Correction for Amino Acid Loss during Acid Hydrolysis of a Purified Protein

Darragh, Alison J.; Garrick, Dorian J.; Moughan, Paul J.; Hendriks, W.H.

doi:10.1006/abio.1996.0157

Cited by 69 publications

(69 citation statements)

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“…However, the amount of aspartic acid recovered, and the good precision of the recovery (6.7%), suggested that asparagine's contribution to the aspartic acid pool was small. The low recoveries of threonine, serine, tyrosine, methionine and cysteine may have been due to their increased lability at low pH (Darragh et al 1996), although any loss of tyrosine may have been masked by the conversion of phenylalanine to tyrosine by ascorbic acid (Metzler 1977). In contrast to previous studies, tryptophan was occasionally evident but recoveries were low (ca.…”

Section: Resultsmentioning

confidence: 98%

Distributions, cycling and recovery of amino acids in estuarine waters and sediments

2007

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Acid hydrolysis of estuarine water samples for the determination of amino acids (AAs) was tested and found to be effective at high (250 lM) nitrate concentrations when the anti-oxidant, ascorbic acid, was added to the samples. Hydrolysable AA concentrations were then determined in surface sediments collected from low and high salinity regions of the Tamar Estuary (UK) during winter 2003 and 2004, and in overlying water when simulated resuspension of sediment particles was performed. Concentrations of AAs in sediment samples comprised <50% of particulate nitrogen, fitting the paradigm that most sedimentary nitrogen is preserved within an organic matrix. When sediment samples were resuspended in overlying water (salinity 17.5), the rapid, measured increase in dissolved AA concentrations almost equalled the reported nitrate concentration in the lower estuary, with the subsequent decrease in the total dissolved AA levels suggested that bacterial uptake was occurring. Our data concur with previous studies on nitrogen desorption from sediments and suggest that an understanding of organic nitrogen cycling will be an important aspect of future effective estuarine management.

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Section: Resultsmentioning

confidence: 98%

Distributions, cycling and recovery of amino acids in estuarine waters and sediments

2007

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“…11 Also, it has been established that valine and isoleucine are released slowly during hydrolysis, while serine and threonine are degraded prior to 24 h hydrolysis. 4,6,12 In the present study, amino acids were greatly released from 2 to 6 h hydrolysis, and valine and isoleucine were not maximized until the ®nal hydrolysis time (66 h).…”

Section: Effects Of Hydrolysis Timementioning

confidence: 99%

Sequential hydrolysis of swine carcass samples and determination of amino acid concentrations using pre-column derivatization with phenyl isothiocyanate

Wubben

Albin

Gabert

2000

J. Sci. Food Agric.

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“…3.2). Of among, complete hydrolysis is critical for overall accuracy of the procedure (Albin et al, 2000;Anderson et al, 1977;Darragh et al, 1996;Fountoulakis and Lahm, 1998;Kinumi et al, 2010). Moreover, for the analysis of an unknown protein, optimization of hydrolysis conditions should be considered before conducting the whole procedure.…”

Section: Amino Acid Analysismentioning

confidence: 99%