Correction: Expression of immunohistochemical markers in non-oropharyngeal head and neck squamous cell carcinoma in Ghana

Owusu-Afriyie, Osei; Owiredu, William K. B. A.; Owusu-Danquah, Kwabena; Komarck, Christine M.; Foltin, Susan K.; Larsen‐Reindorf, Rita; Acheampong, Emmanuel; Quayson, Solomon; Prince, Mark; McHugh, Jonathan B.; Donkor, Peter; Merajver, Sofía D.; Brenner, J. Chad

doi:10.1371/journal.pone.0209696

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“…Future studies using nasospheroids may include an extended time for live-cell observations in some cases with rare CF genotypes 44 , 45 and testing of recently approved modulator drugs, such TRIKAFTA/KAFTRIO 46 .…”

Section: Discussionmentioning

confidence: 99%

Validation of nasospheroids to assay CFTR functionality and modulator responses in cystic fibrosis

Calucho

Gartner

Barranco

et al. 2021

Sci Rep

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The availability of a simple, robust and non-invasive in vitro airway model would be useful to study the functionality of the cystic fibrosis transmembrane regulator (CFTR) protein and to personalize modulator therapy for cystic fibrosis (CF) patients. Our aim was to validate a CFTR functional study using nasospheroids, a patient-derived nasal cell 3D-culture. We performed live-cell experiments in nasospheroids obtained from wild-type individuals and CF patients with different genotypes and phenotypes. We extended the existing method and expanded the analysis to upgrade measurements of CFTR activity using forskolin-induced shrinking. We also tested modulator drugs in CF samples. Immobilizing suspended-nasospheroids provided a high number of samples for live-cell imaging. The diversity observed in basal sizes of nasospheroids did not affect the functional analysis of CFTR. Statistical analysis with our method was simple, making this protocol easy to reproduce. Moreover, we implemented the measurement of inner fluid reservoir areas to further differentiate CFTR functionality. In summary, this rapid methodology is helpful to analyse response to modulators in CF samples to allow individualized treatment for CF patients.

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Section: Discussionmentioning

confidence: 99%