Correction: Establishment and characterization of a primary androgen‐responsive African‐American prostate cancer cell line, E006AA. Prostate 2004;60(2):145‐152

Koochekpour, Shahriar; Maresh, GA; Katner, Adrienne; Parker-Johnson, KiTani A.; Lee, Ta‐Jen; Hebert, FE; Kao, Y.-L.; Skinner, June; Rayford, Walter

doi:10.1002/pros.23800

Cited by 2 publications

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“…2 A and B). While first thought to be a prostate cancer cell line, E006AA-hT cells were later found to be a clone of renal cell carcinoma cell line 786‐O [ 56 ]. The variation of LINE-1 expression in these cell lines provides a compelling model to better understand LINE-1 transcriptional activation and repression in cancer cells.…”

Section: Resultsmentioning

confidence: 99%

Unbiased proteomic mapping of the LINE-1 promoter using CRISPR Cas9

Briggs¹,

Mita²,

Sun³

et al. 2021

Mobile DNA

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Background The autonomous retroelement Long Interspersed Element-1 (LINE-1) mobilizes though a copy and paste mechanism using an RNA intermediate (retrotransposition). Throughout human evolution, around 500,000 LINE-1 sequences have accumulated in the genome. Most of these sequences belong to ancestral LINE-1 subfamilies, including L1PA2-L1PA7, and can no longer mobilize. Only a small fraction of LINE-1 sequences, approximately 80 to 100 copies belonging to the L1Hs subfamily, are complete and still capable of retrotransposition. While silenced in most cells, many questions remain regarding LINE-1 dysregulation in cancer cells. Results Here, we optimized CRISPR Cas9 gRNAs to specifically target the regulatory sequence of the L1Hs 5’UTR promoter. We identified three gRNAs that were more specific to L1Hs, with limited binding to older LINE-1 sequences (L1PA2-L1PA7). We also adapted the C-BERST method (dCas9-APEX2 Biotinylation at genomic Elements by Restricted Spatial Tagging) to identify LINE-1 transcriptional regulators in cancer cells. Our LINE-1 C-BERST screen revealed both known and novel LINE-1 transcriptional regulators, including CTCF, YY1 and DUSP1. Conclusion Our optimization and evaluation of gRNA specificity and application of the C-BERST method creates a tool for studying the regulatory mechanisms of LINE-1 in cancer. Further, we identified the dual specificity protein phosphatase, DUSP1, as a novel regulator of LINE-1 transcription.

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Section: Resultsmentioning

confidence: 99%

Unbiased proteomic mapping of the LINE-1 promoter using CRISPR Cas9

Briggs¹,

Mita²,

Sun³

et al. 2021

Mobile DNA

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“…E006AA cells were kindly provided by Dr. Shahriar Koochekpour (Roswell Park Cancer Institute, Buffalo, NY, USA). These cells were maintained and cultured as described previously [36,37]. Human ovarian adenocarcinoma cell line SKOV3 was obtained from American Type Culture Collection (ATCC) (Rockville, MD, USA) and maintained in Dulbecco's Modified Eagle Medium, supplemented with 5% FBS, in a 5% CO 2 environment at 37 • C.…”

Section: Cell Culturementioning

confidence: 99%

Small Molecule Inhibitors Targeting Gαi2 Protein Attenuate Migration of Cancer Cells

Caggia

Tapadar

et al. 2020

Cancers

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Heterotrimeric G-proteins are ubiquitously expressed in several cancers, and they transduce signals from activated G-protein coupled receptors. These proteins have numerous biological functions, and they are becoming interesting target molecules in cancer therapy. Previously, we have shown that heterotrimeric G-protein subunit alphai2 (Gαi2) has an essential role in the migration and invasion of prostate cancer cells. Using a structure-based approach, we have synthesized optimized small molecule inhibitors that are able to prevent specifically the activation of the Gαi2 subunit, keeping the protein in its inactive GDP-bound state. We observed that two of the compounds (13 and 14) at 10 μΜ significantly inhibited the migratory behavior of the PC3 and DU145 prostate cancer cell lines. Additionally, compound 14 at 10 μΜ blocked the activation of Gαi2 in oxytocin-stimulated prostate cancer PC3 cells, and inhibited the migratory capability of DU145 cells overexpressing the constitutively active form of Gαi2, under basal and EGF-stimulated conditions. We also observed that the knockdown or inhibition of Gαi2 negatively regulated migration of renal and ovarian cancer cell lines. Our results suggest that small molecule inhibitors of Gαi2 have potential as leads for discovering novel anti-metastatic agents for attenuating the capability of cancer cells to spread and invade to distant sites.

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Correction: Establishment and characterization of a primary androgen‐responsive African‐American prostate cancer cell line, E006AA. Prostate 2004;60(2):145‐152

Cited by 2 publications

References 0 publications

Unbiased proteomic mapping of the LINE-1 promoter using CRISPR Cas9

Unbiased proteomic mapping of the LINE-1 promoter using CRISPR Cas9

Small Molecule Inhibitors Targeting Gαi2 Protein Attenuate Migration of Cancer Cells

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