Correction: Differential anti-proliferative and apoptotic effects of lichen species on human prostate carcinoma cells

Göncü, Beyza; Sevgi, Ece; Hançer, Çağla Kizilarslan; Gokay, Guzin; Özten, Nur

doi:10.1371/journal.pone.0244831

Cited by 2 publications

(3 citation statements)

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“…Apoptotic cell ratios were analyzed by flow cytometry using the Muse cell analyzer system, and gating was adjusted according to the untreated sample. Results were presented as the percentage of cells that were viable (Ann-V– 7-AAD−), early apoptotic (Ann-V+ 7-AAD−), late apoptotic (Ann-V+ 7-AAD−), or dead (Ann-V– 7-AAD+) …”

Section: Methodsmentioning

confidence: 99%

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Tail-Approach-Based Design and Synthesis of Coumarin-Monoterpenes as Carbonic Anhydrase Inhibitors and Anticancer Agents

et al. 2023

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In this study, sixty novel coumarin-monoterpene compounds were synthesized in two series [thirty-two compounds (12−43) bearing a triazole ring in the first series, and twenty-eight compounds (44−71) bearing an alkyl chain in the second one]. Their inhibitory effects on the human carbonic anhydrase (hCA) isoforms I, II, IX, and XII and anticancer potentials were determined. All synthesized molecules selectively inhibited CA IX and XII. 23 and 42 were found to be the strongest inhibitors, with K i values of 1.9 nM against hCA IX. Also, 70 showed the highest inhibitory activity with a K i value of 4.9 nM against hCA XII. Moreover, their cytotoxic effects on colon adenocarcinoma (HT-29), prostate adenocarcinoma (PC-3), and breast adenocarcinoma (MCF-7) cell lines were evaluated. According to the cytotoxicity results, 14 (IC 50 = 2.48 μM) and 63 (IC 50 = 3.91 μM) exhibited the highest cytotoxicity on the MCF-7 cells, while 23 showed the strongest cytotoxic effect on both PC-3 (IC 50 = 9.40 μM) and HT-29 (IC 50 = 12.10 μM) cell lines. 14, 23, and 66 decreased CA IX and CA XII protein expression in HT-29 cells, while 23 and 66 showed the strongest reduction of both CA IX and CA XII in MCF-7 cells. All of the selected compounds increased total apoptosis in a concentration-dependent manner in HT-29 and MCF-7 cells. 14 has the strongest apoptotic effect in MCF-7 cells. 23 increased early apoptosis primarily, while 14 and 66 increased total apoptosis in HT-29. In addition, PI/Hoechst staining proves that apoptotic cells are increased in HT-29 with an effect of 14, 23, and 66. As a result of the modeling studies, it has been shown that only the open coumarin form of the compounds can interact directly with the active-site Zn 2+ ion. It has been shown that coumarinmonoterpene structures with different alkyl and monoterpene groups both specifically inhibit CA IX and XII and exhibit specific cytotoxicity in different cell lines.

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Section: Methodsmentioning

confidence: 99%

“…Results were presented as the percentage of cells that were viable (Ann-V– 7-AAD−), early apoptotic (Ann-V+ 7-AAD−), late apoptotic (Ann-V+ 7-AAD−), or dead (Ann-V– 7-AAD+). 26 …”

Section: Methodsmentioning

confidence: 99%

Tail-Approach-Based Design and Synthesis of Coumarin-Monoterpenes as Carbonic Anhydrase Inhibitors and Anticancer Agents

et al. 2023

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“…Results are given as a percentage (%). 43 There are two different dyes in the reagent: Annexin V (apoptosis marker) and 7-AAD (dead cell marker). The profiles of the cells after staining with these dyes according to their viability are as follows:…”

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confidence: 99%

Synthesis of Sorafenib−Ruthenium Complexes, Investigation of Biological Activities and Applications in Drug Delivery Systems as an Anticancer Agent

Zengin Kurt,

Öztürk Civelek,

Çakmak

et al. 2024

J. Med. Chem.

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Sorafenib, a multiple kinase inhibitor, is widely used as a first-line treatment for hepatocellular carcinoma. However, there is a need for more effective alternatives when sorafenib proves insufficient. In this study, we aimed to design a structure that surpasses sorafenib's efficacy, leading us to synthesize sorafenib− ruthenium complexes for the first time and investigate their properties. Our results indicate that the sorafenib−ruthenium complexes exhibit superior epidermal growth factor receptor (EGFR) inhibition compared to sorafenib alone. Interestingly, among these complexes, Ru3S demonstrated high activity against various cancer cell lines including sorafenib-resistant HepG2 cells while exhibiting significantly lower cytotoxicity than sorafenib in healthy cell lines. Further evaluation of cell cycle, cell apoptosis, and antiangiogenic effects, molecular docking, and molecular dynamics studies revealed that Ru3S holds great potential as a drug candidate. Additionally, when free Ru3S was encapsulated into polymeric micelles M1, enhanced cytotoxicity on HepG2 cells was observed. Collectively, these findings position Ru3S as a promising candidate for EGFR inhibition and warrant further exploration for drug development purposes.

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Correction: Differential anti-proliferative and apoptotic effects of lichen species on human prostate carcinoma cells

Cited by 2 publications

References 1 publication

Tail-Approach-Based Design and Synthesis of Coumarin-Monoterpenes as Carbonic Anhydrase Inhibitors and Anticancer Agents

Tail-Approach-Based Design and Synthesis of Coumarin-Monoterpenes as Carbonic Anhydrase Inhibitors and Anticancer Agents

Synthesis of Sorafenib−Ruthenium Complexes, Investigation of Biological Activities and Applications in Drug Delivery Systems as an Anticancer Agent

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