Correction: Corrigendum: The Mouse Intestinal Bacterial Collection (miBC) provides host-specific insight into cultured diversity and functional potential of the gut microbiota

Lagkouvardos, Ilias; Baines, JF; Stecher, Bärbel; Peterson, Danielle; Abt, Birte; Plugge, Caroline M.; Ziegler, Caroline; Meier‐Kolthoff, Jan P.; Clavel, Thomas; Bui, Thi Phuong Nam; Overmann, Jörg; Garzetti, Debora; Nielsen, Henrik Bjørn; Brugiroux, Sandrine; Hugenholtz, Floor; Smidt, Hauke; Haller, Dirk; Martínez, Inés; Hornef, Mathias; Kumar, Nitin; Bresciani, Anne Gøther; Pukall, Rüdiger; Foesel, Bärbel U.; Just, Sarah; Wang, J.; Wenning, Mareike; Kristiansen, Karsten; Walter, Jens

doi:10.1038/nmicrobiol.2016.219

Cited by 29 publications

(17 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Of these three species, L. murinus is the only species that was part of the Altered Schaedler Formula, the community of microbes that were used to originally colonize gnotobiotic mice to develop SPF laboratory mice as we know them today (29,30). Similar examples of host specificity have also been reported in other mouse microbiomes, including the colonization of segmented filamentous bacteria (31) and Muribaculaceae (19) in the mouse gut microbiome. This specificity also strongly indicates to the phenomenon of host-microbe coevolution and fitness characteristics (32).…”

Section: Discussionmentioning

confidence: 64%

“…Similar databases have already been developed to characterize the oral microbiome in humans (15,16) and other mammalian host species such as cats and dogs (17,18). More recently, researchers have characterized and generated a database for the mouse intestinal bacterial community (19,20), as well as chronicled the collection of genes in the mouse gut metagenome (21).…”

mentioning

confidence: 99%

See 1 more Smart Citation

A 16S rRNA Gene and Draft Genome Database for the Murine Oral Bacterial Community

et al. 2021

View full text Add to dashboard Cite

A curated murine oral microbiome database to be used as a reference for mouse-based studies has been constructed using a combination of bacterial culture, 16S rRNA gene amplicon, and whole-genome sequencing. The database comprises a collection of nearly full-length 16S rRNA gene sequences from cultured isolates and draft genomes from representative taxa collected from a range of sources, including specific-pathogen-free laboratory mice, wild Mus musculus domesticus mice, and formerly wild wood mouse Apodemus sylvaticus. At present, it comprises 103 mouse oral taxa (MOT) spanning four phyla—Firmicutes, Proteobacteria, Actinobacteria, and Bacteroidetes—including 12 novel undescribed species-level taxa. The key observations from this study are (i) the low diversity and predominantly culturable nature of the laboratory mouse oral microbiome and (ii) the identification of three major murine-specific oral bacterial lineages, namely, Streptococcus danieliae (MOT10), Lactobacillus murinus (MOT93), and Gemella species 2 (MOT43), which is one of the novel, still-unnamed taxa. Of these, S. danieliae is of particular interest, since it is a major component of the oral microbiome from all strains of healthy and periodontally diseased laboratory mice, as well as being present in wild mice. It is expected that this well-characterized database should be a useful resource for in vitro experimentation and mouse model studies in the field of oral microbiology. IMPORTANCE Mouse model studies are frequently used in oral microbiome research, particularly to investigate diseases such as periodontitis and caries, as well as other related systemic diseases. We have reported here the details of the development of a curated reference database to characterize the oral microbial community in laboratory and some wild mice. The genomic information and findings reported here can help improve the outcomes and accuracy of host-microbe experimental studies that use murine models to understand health and disease. Work is also under way to make the reference data sets publicly available on a web server to enable easy access and downloading for researchers across the world.

show abstract

Section: Discussionmentioning

confidence: 64%

mentioning

confidence: 99%

A 16S rRNA Gene and Draft Genome Database for the Murine Oral Bacterial Community

et al. 2021

View full text Add to dashboard Cite

show abstract

“…6). Members of Muribaculaceae are well adapted to the mouse gut microbiome (52) and may comprise up to 685 species-level clusters (53). Further, analysis of the 157 draft genomes of the members of Muribaculaceae showed them to be highly enriched in glycoside hydrolases, suggesting an ability to deconstruct complex carbohydrates such as MACs and thereby promote SCFA production (53,54).…”

Section: Effect Of a High-fat/low-protein Keto-like Diet On Antibiotimentioning

confidence: 99%

A High-Fat/High-Protein, Atkins-Type Diet Exacerbates Clostridioides ( Clostridium ) difficile Infection in Mice, whereas a High-Carbohydrate Diet Protects

et al. 2020

View full text Add to dashboard Cite

Clostridioides difficile (formerly Clostridium difficile) infection (CDI) can result from the disruption of the resident gut microbiota. Western diets and popular weight-loss diets drive large changes in the gut microbiome; however, the literature is conflicted with regard to the effect of diet on CDI. Using the hypervirulent strain C. difficile R20291 (RT027) in a mouse model of antibiotic-induced CDI, we assessed disease outcome and microbial community dynamics in mice fed two high-fat diets in comparison with a high-carbohydrate diet and a standard rodent diet. The two high-fat diets exacerbated CDI, with a high-fat/high-protein, Atkins-like diet leading to severe CDI and 100% mortality and a high-fat/low-protein, medium-chain-triglyceride (MCT)-like diet inducing highly variable CDI outcomes. In contrast, mice fed a high-carbohydrate diet were protected from CDI, despite the high levels of refined carbohydrate and low levels of fiber in the diet. A total of 28 members of the Lachnospiraceae and Ruminococcaceae decreased in abundance due to diet and/or antibiotic treatment; these organisms may compete with C. difficile for amino acids and protect healthy animals from CDI in the absence of antibiotics. Together, these data suggest that antibiotic treatment might lead to loss of C. difficile competitors and create a favorable environment for C. difficile proliferation and virulence with effects that are intensified by high-fat/high-protein diets; in contrast, high-carbohydrate diets might be protective regardless of the source of carbohydrate or of antibiotic-driven loss of C. difficile competitors. IMPORTANCE The role of Western and weight-loss diets with extreme macronutrient composition in the risk and progression of CDI is poorly understood. In a longitudinal study, we showed that a high-fat/high-protein, Atkins-type diet greatly exacerbated antibiotic-induced CDI, whereas a high-carbohydrate diet protected, despite the high monosaccharide and starch content. Our study results, therefore, suggest that popular high-fat/high-protein weight-loss diets may enhance CDI risk during antibiotic treatment, possibly due to the synergistic effects of a loss of the microorganisms that normally inhibit C. difficile overgrowth and an abundance of amino acids that promote C. difficile overgrowth. In contrast, a high-carbohydrate diet might be protective, despite reports on the recent evolution of enhanced carbohydrate metabolism in C. difficile.

show abstract

“…The Bacteroidetes family Muribaculaceae has been proposed only recently (1), and few isolates and/or genome sequences are publicly available from members of this family. This regards specifically the Muribaculaceae genus Duncaniella (2), which has currently only one cultured representative, the D.…”

Section: Announcementmentioning

confidence: 99%

Complete Genome Sequence of Duncaniella muris Strain B8, Isolated from the Feces of C57/BL6 Mice

Miyake

Ding

Soh

et al. 2019

Microbiol Resour Announc

View full text Add to dashboard Cite

show abstract

Correction: Corrigendum: The Mouse Intestinal Bacterial Collection (miBC) provides host-specific insight into cultured diversity and functional potential of the gut microbiota

Cited by 29 publications

References 0 publications

A 16S rRNA Gene and Draft Genome Database for the Murine Oral Bacterial Community

A 16S rRNA Gene and Draft Genome Database for the Murine Oral Bacterial Community

A High-Fat/High-Protein, Atkins-Type Diet Exacerbates Clostridioides ( Clostridium ) difficile Infection in Mice, whereas a High-Carbohydrate Diet Protects

Complete Genome Sequence of Duncaniella muris Strain B8, Isolated from the Feces of C57/BL6 Mice

Contact Info

Product

Resources

About