Correction: Corrigendum: Endogenous Arabidopsis messenger RNAs transported to distant tissues

Abstract

In the version of this Article originally published, the x-axis scale in Fig. 4b was incorrectly labelled and should have ranged from-10 to 10. This has been corrected in all versions of the Article.

The mRNA mobileome: challenges and opportunities for deciphering signals from the noise

“…Despite strong evidence in support of phloem-based movement, consistent evidence has shown that up to a quarter of identified mobile transcripts move against the source-to-sink gradient (from root to shoot in most cases). This observation conflicts with the assumption that the mRNA mobileome is transported via bulk phloem flow ( Ruiz-Medrano et al 1999 ; Notaguchi et al 2015 ; Yang et al 2015 ; Thieme et al 2016 ; Zhang et al 2016b ; Li et al 2021 ). These findings imply that mRNAs are likely moving against source-to-sink within the phloem, although no mechanism for this movement has been described ( Thieme et al 2015 ; Kehr and Kragler 2018 ).…”

“…Since this initial discovery, mRNAs have been found in the phloem sap of a broad range of families, including the Brassicaceae, Cucurbitaceae, and Euphorbiaceae ( Doering-Saad et al 2002 , 2006 ; Omid et al 2007 ; Deeken et al 2008 ; Kanehira et al 2010 ; Dinant and Kehr 2013 ; Ostendorp et al 2017 ). These observations of mRNA in the phloem sap have subsequently been expanded upon and now demonstrate that RNA movement is associated with diverse processes, including gene silencing, development, abiotic responses, and plant–parasite interactions ( Kim et al 2001 ; Haywood et al 2005 ; Banerjee et al 2006 ; David-Schwartz et al 2008 ; Molnar et al 2010 ; Melnyk et al 2011 ; Pallas and Gómez 2013 ; Kim et al 2014 ; Lewsey et al 2016 ; Thieme et al 2016 ; Ghate et al 2017 ; Shahid et al 2018 ; Johnson et al 2019 ). Indeed, across a wide range of studies, there is a ubiquitous presence of long-distance mobile RNAs moving throughout the plant body.…”

“…First, phloem sap has been shown to protect RNA from degradation ( Sasaki et al 1998 ; Doering-Saad et al 2002 ; Gaupels et al 2008 ), while xylem sap contains RNases ( Alvarez et al 2006 ; Bai et al 2013 ). Second, most currently published studies profiling long-distance mRNA mobility show a strong source-to-sink relationship of movement ( Ruiz-Medrano et al 1999 ; Notaguchi et al 2015 ; Yang et al 2015 ; Thieme et al 2016 ; Zhang et al 2016b ; Li et al 2021 ). Third, modeling of factors that influence RNA entry into the phloem shows that proximity of transcript expression to sieve elements correlates with long-distance movement ( Calderwood et al 2016 ).…”