Correction: Comparative evaluation of a new magnetic bead-based DNA extraction method from fecal samples for downstream next-generation 16S rRNA gene sequencing

McGaughey, Kara D.; Yilmaz-Swenson, Tulay; Elsayed, Nourhan M.; Cruz, Dianne A.; Rodriguez, Ramona M.; Kritzer, Michael D.; Peterchev, Angel V.; Gray, Megan; Lewis, Samantha R.; Roach, Jeffrey; Wetsel, William C.; Williamson, Douglas E.

doi:10.1371/journal.pone.0212712

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“…Microbial DNA in mouse fecal samples was extracted using the CTAB method [29]. The extracted samples were sequenced using the 16S rRNA gene sequencing method [30].…”

Section: Dna Extraction and Sequencingmentioning

confidence: 99%

Effects of Different Heat Treatments on Yak Milk Proteins on Intestinal Microbiota and Metabolism

Shu,

Jing,

et al. 2024

Foods

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Dairy products are susceptible to modifications in protein oxidation during heat processing, which can lead to changes in protein function, subsequently affecting intestinal health. Despite being a unique nutritional source, yak milk has not been thoroughly examined for the effects of its oxidized proteins on intestinal microbiota and metabolism. Hence, this study employed different heat treatment methods (low-temperature pasteurization, high-temperature pasteurization, and high-temperature sterilization) to induce oxidation in yak milk proteins. The study then assessed the degree of oxidation in these proteins and utilized mice as research subjects. Using metagenomics and metabolomics methods, this study examined the structure of intestinal microbial communities and metabolic products in mice consuming oxidized yak milk. The results showed a decrease in carbonyl and total thiol contents of yak milk proteins after different heat treatments, indicating that heat treatment causes oxidation in yak milk proteins. Metagenomic analysis of mouse intestinal microbiota revealed significant changes in 66 genera. In the high-temperature sterilization group (H), key differential genera included Verrucomicrobiales, Verrucomicrobiae, Akkermansiaceae, and 28 others. The high-temperature pasteurization group (M) mainly consisted of Latilactobacillus, Bacillus, and Romboutsia. The low-temperature pasteurization group (L) primarily comprised of Faecalibacterium, Chaetomium, Paenibacillaceae, Eggerthella, Sordariales, and 33 others. Functionally, compared to the control group (C), the H group upregulated translation and energy metabolism functions, the L group the M group significantly upregulated metabolism of other amino acids, translation, and cell replication and repair functions. Based on metabolomic analysis, differential changes in mouse metabolites could affect multiple metabolic pathways in the body. The most significantly affected metabolic pathways were phenylalanine metabolism, vitamin B6 metabolism, steroid hormone biosynthesis, and pantothenate and CoA biosynthesis. The changes were similar to the functional pathway analysis of mouse metagenomics, affecting amino acid and energy metabolism in mice. In summary, moderate oxidation of yak milk proteins exhibits a positive effect on mouse intestinal microbiota and metabolism. In conclusion, yak milk has a positive effect on mouse intestinal microflora and metabolism, and this study provides a scientific basis for optimizing dairy processing technology and further developing and applying yak milk.

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“…Microbial DNA in mouse fecal samples was extracted using the CTAB method [29]. The extracted samples were sequenced using the 16S rRNA gene sequencing method [30].…”

Section: Dna Extraction and Sequencingmentioning

confidence: 99%

Effects of Different Heat Treatments on Yak Milk Proteins on Intestinal Microbiota and Metabolism

Shu,

Jing,

et al. 2024

Foods

View full text Add to dashboard Cite

show abstract

Correction: Comparative evaluation of a new magnetic bead-based DNA extraction method from fecal samples for downstream next-generation 16S rRNA gene sequencing

Cited by 1 publication

References 1 publication

Effects of Different Heat Treatments on Yak Milk Proteins on Intestinal Microbiota and Metabolism

Effects of Different Heat Treatments on Yak Milk Proteins on Intestinal Microbiota and Metabolism

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