Correction: Broad-range lytic bacteriophages that kill Staphylococcus aureus local field strains

Abatángelo, Virginia; Bacci, Natalia Peressutti; Boncompain, Carina Andrea; Amadío, Ariel Fernando; Carrasco, Soledad Telma; Suárez, Cristian Alejandro; Morbidoni, Héctor Ricardo

doi:10.1371/journal.pone.0187387

Cited by 16 publications

(9 citation statements)

References 1 publication

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“…However, the potential problems to the use of this kind of therapy must be carefully considered like narrow host range of the phages, the development of resistance of the host bacteria to phages during therapy, potential of inactivation of phages by the patient's immune system, and safety of phage application in humans. 34 Although there are also studies suggesting the use of bacteriophages in the treatment of infection in humans, such as S aureus, P aeruginosa, E coli, Salmonella typhi, and so on, 35 the studies showing the efficacy of bacteriophages in the treatment of chronic nonhealing wound are very few. The encouraging results of the present study indicate the potential role of bacteriophage therapy in the future.…”

Section: Discussionmentioning

confidence: 99%

“…Although there are also studies suggesting the use of bacteriophages in the treatment of infection in humans, such as S aureus, P aeruginosa, E coli, Salmonella typhi , and so on, 35 the studies showing the efficacy of bacteriophages in the treatment of chronic nonhealing wound are very few. The encouraging results of the present study indicate the potential role of bacteriophage therapy in the future.…”

Section: Discussionmentioning

confidence: 99%

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Use of Customized Bacteriophages in the Treatment of Chronic Nonhealing Wounds: A Prospective Study

Patel

Bhartiya

Kumar

et al. 2019

The International Journal of Lower Extremity Wounds

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Nonhealing ulcers are a great challenge to surgeons as they may occasionally culminate in amputation of the affected part. Mostly nonhealing of wounds results due to infection by antibiotic-resistant bacteria and subsequent biofilm formation. However, customized bacteriophage therapy may take care of both of the above-mentioned hurdles. A total of 48 study subjects of age group 12 to 70 years, having minimum one eligible full-thickness wound and failed to heal in 6-week duration with conventional therapy, were included in this exploratory prospective study. Patients with systemic diseases, that is, burn, malignancy, dermatological disorders, and ulcers with leprosy or tuberculosis, were excluded. However, subjects having diabetes and hypertension were included in the study. The customized monophage for single bacterial infection and cocktail of phages specific to 2 or more infecting bacteria were applied on an alternate day over the wound surface. A total of 5 to 7 applications were made till the wound became free of infecting bacteria. The study period extended from August 2018 to May 2019. The study subjects were followed for 3 months since the start of therapy. A cure rate of 81.2% could be obtained, of which 90.5% (19/21) patients were nondiabetic and 74.1% (20/27) diabetic. The wounds infected with Klebsiella pneumoniae were observed with relatively delayed healing. Post phage therapy, the mean hemoglobin level and percentage of lymphocytes increased significantly. The customized local phage therapy is very promising in nonhealing ulcers.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Use of Customized Bacteriophages in the Treatment of Chronic Nonhealing Wounds: A Prospective Study

Patel

Bhartiya

Kumar

et al. 2019

The International Journal of Lower Extremity Wounds

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“…Staphylococcus aureus is a common commensal bacterium that, as an opportunistic pathogen, is capable of causing a variety of diseases ranging from mild skin infections and food poisoning to fatal infections like endocarditis, pneumonia, osteomyelitis, and toxic shock syndrome. It is also a leading cause of infections associated with catheters and devices (8)(9)(10)(11). The pathogenesis of S. aureus strains are related to the expression of different virulence factors, including cell surface components (e.g., collagen-binding protein, clumping factor, fibronectinbinding protein, and elastin binding protein) and secreted factors (e.g., staphylokinase, toxic shock syndrome toxin-1, hemolysin, panton-valentine leukocidin (PVL), exfoliative toxins (eta and etb), staphylococcal enterotoxins (SEs), and lipase) (12).…”

Section: Introductionmentioning

confidence: 99%

“…This approach uses bacterial viruses (phages) that can specifically attack pathogenic bacteria and kill them (20). Phage therapy has the potential of being highly specific merely against a species or even a strain responsible for infection (8,21,22). Moreover, their remarkable specificity pre-vents them from affecting human cells and the composition of the body microbiota and inducing antimicrobial resistance in different bacterial species (1,22).…”

Section: Introductionmentioning

confidence: 99%

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Characterizing a Lytic Bacteriophage Infecting Methicillin-Resistant Staphylococcus aureus (MRSA) Isolated From Burn Patients

Hallajzadeh

Mojtahedi

Amirmozafari

et al. 2020

Arch Clin Infect Dis

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Background: Methicillin-resistant Staphylococcus aureus (MRSA) is a significant threat to human health. It is a multidrug-resistant (MDR) pathogen capable of causing a variety of diseases. Also, MRSA is one of the most important nosocomial pathogens in burn infection. As a treatment strategy against MRSA infections, phage therapy has the potential of becoming an alternative remedy. Objectives: The present study aimed to isolate and characterize a lytic bacteriophage from hospital sewage to be effective against burn wound-infecting MRSA isolates. Methods: Staphylococcus aureus strains were isolated from hospitalized burn patients. The strains were confirmed as MRSA by the Kirby-Bauer disk diffusion method using penicillin, methicillin, and oxacillin, as well as the PCR assay for the mecA gene. The phage was isolated from the hospital sewage and tittered by the double layer agar (DLA) method. The spot test was used for host range determination. The latent period and burst size were estimated from a one-step growth curve. The phage morphology was observed by electron microscopy. The nature of the nucleic acid of the isolated bacteriophages was confirmed by Rnase A, Dnase I, and six restriction enzymes. Results: The titer, latent period, and burst size of the isolated phage were determined to be 1 × 10 9 PFU/mL, 20 min, and 190 PFU per infected cell, respectively. It displayed a broad host range for MRSA bacteria by the spot test (27 out of 30 isolates). Electron microscopy observation demonstrated that the phage belonged to the Myoviridea family. Digestion profiles of Rnase A, Dnase I, and six restriction enzymes in 1% agarose gel electrophoresis showed that the genome of the isolated phage was a double-stranded DNA with a size of <~23 kbp. Conclusions: The isolated phage (MH-1) was active against a wide range of MRSA strains recovered from burn patients. Its specificity and remarkable lytic effects on MRSA strains emphasized that it could be a suitable candidate for use in prophylaxis and treatment of these clinical infections.

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Exploring the virulence potential of immune evasion cluster genes in methicillin-resistant Staphylococcus aureus from cancer patients

Bano,

Asghar,

Ejaz

et al. 2023

Saudi Journal of Biological Sciences

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Correction: Broad-range lytic bacteriophages that kill Staphylococcus aureus local field strains

Cited by 16 publications

References 1 publication

Use of Customized Bacteriophages in the Treatment of Chronic Nonhealing Wounds: A Prospective Study

Use of Customized Bacteriophages in the Treatment of Chronic Nonhealing Wounds: A Prospective Study

Characterizing a Lytic Bacteriophage Infecting Methicillin-Resistant Staphylococcus aureus (MRSA) Isolated From Burn Patients

Exploring the virulence potential of immune evasion cluster genes in methicillin-resistant Staphylococcus aureus from cancer patients

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