Correction: A benzylic linker promotes methyltransferase catalyzed norbornene transfer for rapid bioorthogonal tetrazine ligation

Abstract

Correction for ‘A benzylic linker promotes methyltransferase catalyzed norbornene transfer for rapid bioorthogonal tetrazine ligation’ by F. Muttach et al., Chem. Sci., 2017, DOI: ; .

Development of a Universal RNA Dual-Terminal Labeling Method for Sensing RNA-Ligand Interactions

“…RNA single-labeling strategies have been successfully developed during the past few years [14][15][16][17][18][19][20][21][22][23][24][25][26][27][28] . In general, chemo-enzymatic approaches are used to directly install various labels (such as alkyne or azide) into different ncRNAs for the second-step fast click modifications of various functional groups 17,18 . In addition, direct chemical reaction of RNA at 3'-terminus can be achieved based on periodate chemistry 25 .…”

“…RNA 5'-terminus can be chemically labeled by several methods [14][15][16][17][18][19][20][21][22] . For example, direct 5'-functionalization with diazo reagents was achieved for short RNAs 20 , while the use toward long RNAs needs to be further validated.…”

Light-control of cap methylation and mRNA translation via genetic code expansion of Ecm1

“…To achieve this, we chose the guanine N7 MTase Ecm1 that can be efficiently produced in E. coli and that we used previously to generate functional 5 0 caps. 30,[32][33][34][35] We sought to inhibit the activity of Ecm1 by incorporating sterically demanding but photo-cleavable groups in the active site. To generate such "caged" proteins, the genetic code can be expanded, allowing for the site-specic incorporation of non-natural "caged" amino acids into proteins.…”