Correcting the effect of hematocrit in whole blood coagulation analysis on paper-based lateral flow device

Li, Hua; Han, Daewoo; Pauletti, Giovanni M.; Hegener, Michael; Steckl, A. J.

doi:10.1039/c8ay00192h

Cited by 12 publications

(9 citation statements)

References 28 publications

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“…Non-specific binding by plasma constituents in such complex matrices can yield false positive or false negative results [ 96 ]. Variable hematocrit levels can impact the rate of blood flow on the assay as a higher percentage of red blood cells can result in faster coagulation and therefore less fluid movement [ 97 , 98 ]. For example, blood samples from people with anemia or who are on anticoagulants would migrate faster than those with more viscous blood which may change the binding characteristics and assay sensitivity.…”

Section: Challenges With Serology Assay Development and Implementatiomentioning

confidence: 99%

Technical considerations to development of serological tests for SARS-CoV-2

Ernst

Wolfe

Stahura

et al. 2021

Talanta

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Section: Challenges With Serology Assay Development and Implementatiomentioning

confidence: 99%

Technical considerations to development of serological tests for SARS-CoV-2

Ernst

Wolfe

Stahura

et al. 2021

Talanta

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“…Whole blood tests are considered the “gold standard” in clinical diagnostics . Because the intense red color of hemoglobin interferes with the readout of colorimetric or optical detection-based diagnostic tests, plasma separation is a common first step to increase diagnostic test accuracy. − The traditional method for separating plasma from whole blood in lab settings is centrifugation. Plasma separation in both traditional blood centrifuges and in lab-on-CD platforms , require bulky equipment, which limits their application in the point-of-care setting.…”

mentioning

confidence: 99%

Synthetic Paper Separates Plasma from Whole Blood with Low Protein Loss

2020

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The separation of plasma from whole blood is the first step in many diagnostic tests. Point-of-care tests often rely on integrated plasma filters, but protein retention in such filters limits their performance. Here, we investigate plasma separation on interlocked micropillar scaffolds (“synthetic paper”) by the local agglutination of blood cells coupled with the capillary separation of the plasma. We separated clinically relevant volumes of plasma with high efficiency in a separation time on par with that of state of the art techniques. We investigated different covalent and noncovalent surface treatments (PEGMA, HEMA, BSA, O2 plasma) on our blood filter and their effect on protein recovery and identified O2 plasma treatment and 7.9 μg/cm2 agglutination antibody as most suitable treatments. Using these treatments, we recovered at least 82% of the blood plasma proteins, more than with state-of-the-art filters. The simplicity of our device and the performance of our approach could enable better point-of-care tests.

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“…In fact, it was found that the respective LFA proved to be slightly superior in detecting very weak or very strong coagulation as compared to CoaData 2000 Fibrintimer data. The correlation of hematocrit with that of coagulation time was, however, a factor affecting the PT results, which were further addressed and corrected by the author in the LFA device in their consequent studies [ 92 ]. The LFA device after hematocrit correction provided acceptable PT measures when compared and correlated to the results of the standard CoaguChek ® XS coagulometer.…”

Section: Point Of Care Pt/inrmentioning

confidence: 99%

Evolving Paradigm of Prothrombin Time Diagnostics with Its Growing Clinical Relevance towards Cardio-Compromised and COVID-19 Affected Population

Saha

Bajpai

Krishna

et al. 2021

Sensors

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Prothrombin time (PT) is a significant coagulation (hemostasis) biomarker used to diagnose several thromboembolic and hemorrhagic complications based on its direct correlation with the physiological blood clotting time. Among the entire set of PT dependents, candidates with cardiovascular ailments are the major set of the population requiring lifelong anticoagulation therapy and supervised PT administration. Additionally, the increasing incidence of COVID affected by complications in coagulation dynamics has been strikingly evident. Prolonged PT along with sepsis-induced coagulopathy (SIC score > 3) has been found to be very common in critical COVID or CAC-affected cases. Considering the growing significance of an efficient point-of-care PT assaying platform to counter the increasing fatalities associated with cardio-compromised and coagulation aberrations propping up from CAC cases, the following review discusses the evolution of lab-based PT to point of care (PoC) PT assays. Recent advances in the field of PoC PT devices utilizing optics, acoustics, and mechanical and electrochemical methods in microsensors to detect blood coagulation are further elaborated. Thus, the following review holistically aims to motivate the future PT assay designers/researchers by detailing the relevance of PT and associated protocols for cardio compromised and COVID affected along with the intricacies of previously engineered PoC PT diagnostics.

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Correcting the effect of hematocrit in whole blood coagulation analysis on paper-based lateral flow device

Abstract: A simple method to correct the effect of hematocrit (Hct) on a paper-based lateral flow assay device that is designed to assess whole blood coagulation is reported.

Cited by 12 publications

References 28 publications

Technical considerations to development of serological tests for SARS-CoV-2

Technical considerations to development of serological tests for SARS-CoV-2

Synthetic Paper Separates Plasma from Whole Blood with Low Protein Loss

Evolving Paradigm of Prothrombin Time Diagnostics with Its Growing Clinical Relevance towards Cardio-Compromised and COVID-19 Affected Population

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