Corrected Emission Spectra and Quantum Yields for a Series of Fluorescent Compounds in the Visible Spectral Region

Velapoldi, R. A.; Tønnesen, Hanne

doi:10.1023/b:jofl.0000031828.96368.c1

Cited by 288 publications

(187 citation statements)

References 4 publications

Supporting

Mentioning

179

Contrasting

Order By: Relevance

“…em ϭ 555 nm ( ex ϭ 480 nm) [35] Fluorescence quenching (fluorescence relative to free chromophore ϭ 0.05) [35] m-Amsacrine 490.5 nm [38] em ϭ 515 nm [38], ⌽ ϭ 0.92 [38,39] ϭ 4.16 ns [39] Covalently linked (5= end)…”

Section: Minor Groovementioning

confidence: 99%

Electron photodetachment dissociation of DNA anions with covalently or noncovalently bound chromophores

Gabelica

Rosu

Pauw

et al. 2007

J. Am. Soc. Mass Spectrom.

View full text Add to dashboard Cite

Double stranded DNA multiply charged anions coupled to chromophores were subjected to UV-Vis photoactivation in a quadrupole ion trap mass spectrometer. The chromophores included noncovalently bound minor groove binders (activated in the near UV), noncovalently bound intercalators (activated with visible light), and covalently linked fluorophores and quenchers (activated at their maximum absorption wavelength). We found that the activation of only chromophores having long fluorescence lifetimes did result in efficient electron photodetachment from the DNA complexes. In the case of ethidium-dsDNA complex excited at 500 nm, photodetachment is a multiphoton process. The MS 3 fragmentation of radicals produced by photodetachment at ϭ 260 nm (DNA excitation) and by photodetachment at Ͼ 300 nm (chromophore excitation) were compared. The radicals keep no memory of the way they were produced. A weakly bound noncovalent ligand (m-amsacrine) allowed probing experimentally that a fraction of the electronic internal energy was converted into vibrational internal energy. This fragmentation channel was used to demonstrate that excitation of the quencher DABSYL resulted in internal conversion, unlike the fluorophore 6-FAM. Altogether, photodetachment of the DNA complexes upon chromophore excitation can be interpreted by the following mechanism: (1) ligands with sufficiently long excited-state lifetime undergo resonant two-photon excitation to reach the level of the DNA excited states, then (2) the excited-state must be coupled to the DNA excited states for photodetachment to occur. Our experiments also pave the way towards photodissociation probes of biomolecule conformation in the gas-phase by Since then, some major advances must be mentioned in electron activation methods [3][4][5] and in infrared photodissociation [6].We recently started exploring the gas-phase reaction pathways of multiply charged DNA single strands and double strands upon UV irradiation around 260 nm [7,8]. To our surprise, we found out that, instead of fragmentation, electron detachment was the major reaction pathway with strands containing guanines. Electron photodetachment itself is not useful for DNA structure analysis, but subsequent collisional activation of the oligonucleotide radicals produced by electron photodetachment gives fragmentation into w, d, a· and z· ions with good sequence coverage [7]. This technique combining electron photodetachment and collision-induced dissociation was coined electron photodetachment dissociation (EPD). It has now been shown to apply to peptides and proteins as well [9].Apart from the sequencing applications of EPD, numerous questions remain about the electron photodetachment mechanism, and how it compares with electron detachment dissociation [3][4][5] and thermal electron detachment [10,11]. We will briefly summarize our current understanding of the photodetachment mechanism [8]. The electron binding energy in multiply charged DNA anions depends on the balance between electron binding energy of the different DNA...

show abstract

Section: Minor Groovementioning

confidence: 99%

Electron photodetachment dissociation of DNA anions with covalently or noncovalently bound chromophores

Gabelica

Rosu

Pauw

et al. 2007

J. Am. Soc. Mass Spectrom.

View full text Add to dashboard Cite

show abstract

“…The solvent was evaporated to obtain a white solid with a low melting point (4.9 g, 50 % yield). 1 Preparation of monohydroxy-3,6,7,10,11-pentakis(dodecyloxy)triphenylene (C). The monohydroxy derivative C was synthesized as reported for similar systems.…”

mentioning

confidence: 99%

Alignment of Palladium Complexes into Columnar Liquid Crystals Driven by Peripheral Triphenylene Substituents

et al. 2014

View full text Add to dashboard Cite

A new triphenylene-imine (ImH) and its orthopalladated complexes (μ-X) 2 [Pd 2 Im 2 ] (X = CH 3 COO − , Cl − , Br − ), (μ-Cl)(μ-SC n H 2n+1 )[Pd 2 Im 2 ] (n = 6, 12), [PdIm(acac)] [ P d I m C l ( C N C 6 H 4 O C 1 2 H 2 5 ) ] , [ P d I m C l -(CNC 6 H 3 (OC 12 H 25 ) 2 )], and [PdImCl(CNC 6 H 2 (OC 12 H 25 ) 3 )]have been prepared. The free imine ligand is not a liquid crystal, but most ortho-metalated dinuclear palladium complexes and the mononuclear trialkoxyphenyl isocyanide derivative display columnar mesophases at temperatures close to ambient. For the dimeric complexes the mesophase obtained is always columnar rectangular (Col r ), with an uncommon structure: the dimeric triphenylene−Pd complex− triphenylene molecules give rise to a triple-column stacking consisting of two columns of stacked triphenylene groups connected to a central column formed by stacking of two ortho-palladated dimeric moieties. For the trialkoxyphenyl isocyanide derivative a related polymer-like arrangement of columns alternating stacking of triphenylenes with stacking of two ortho-palladated dimeric moieties is found. The mesophase structure is columnar oblique (Col ob ). The free imine and all palladium complexes exhibit fluorescence at room temperature in dichloromethane solution, associated with the triphenylene core.

show abstract

“…The corrected spectrum is calculated by dividing the measured spectra for the obtained correction curve. For quantum yield measurements, the corrected spectrum in energy units must be multiplied by the wavelength to obtain the corrected emission spectrum in photon units (E p ) [5,8]:…”

Section: Methodsmentioning

confidence: 99%

Correction of fluorescence spectra of organic compound solutions

Kovalevskij¹

2006

Lithuanian J. Phys.

View full text Add to dashboard Cite

Fluorescence spectra of various dilute solutions not influenced by the apparatus were obtained using appropriate correction curves. Moreover, the validity of the obtained correction curves is checked by comparing measured fluorescence spectra of standard organic dyes with their spectra reported in various sources. The corrected fluorescence spectra of newly synthesized 3,4,9,10-perylenetetracarboxylic acid derivatives are presented. The observed influence of the changes in the chemical structure of the side groups on their optical spectra is explained by the appearance of the charge transfer state below the first excited singlet state in N,N'-bis(4-diphenylaminophenyl)-3,4,9,10-perylenetetracarboxylic acid diimide (BDFAPhPI) solution, resulting in a huge decrease of the fluorescence efficiency compared to the N,N'-diphenyl-3,4,9,10-perylenetetracarboxylic acid diimide (BPhPI) solution.

show abstract

Corrected Emission Spectra and Quantum Yields for a Series of Fluorescent Compounds in the Visible Spectral Region

Cited by 288 publications

References 4 publications

Electron photodetachment dissociation of DNA anions with covalently or noncovalently bound chromophores

Electron photodetachment dissociation of DNA anions with covalently or noncovalently bound chromophores

Alignment of Palladium Complexes into Columnar Liquid Crystals Driven by Peripheral Triphenylene Substituents

Correction of fluorescence spectra of organic compound solutions

Contact Info

Product

Resources

About