Corporeal Structural and Vascular Micro Architecture With X-Ray Micro Computerized Tomography in Normal and Diabetic Rabbits: Histopathological Correlation

Simopoulos, Demetrios N.; Gibbons, Simon J.; Malysz, John; Szurszewski, Joseph H.; Farrugia, Gianrico; Ritman, Erik L.; Moreland, Robert B.; Nehra, Ajay

doi:10.1016/s0022-5347(05)66413-0

Cited by 36 publications

(25 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In experimental studies, it was found that diabetic animals showed reduction in penile vascular tissue compared with controls [27]. Although the findings from the current study revealed that subendothelial fibrosis among diabetic and nondiabetic patients was not statistically different, in agreement with findings from the study by Simopoulos et al [27]. Although the findings from the current study revealed that subendothelial fibrosis among diabetic and nondiabetic patients was not statistically different, in agreement with findings from the study by Simopoulos et al [27].…”

Section: Discussionsupporting

confidence: 90%

See 1 more Smart Citation

Morphological assessment of corpora cavernosa tissue in diabetic and nondiabetic patients with erectile dysfunction

Mahran¹,

Mohammed²,

Radwan³

et al. 2014

Human Andrology

View full text Add to dashboard Cite

PurposeDiabetes mellitus-induced erectile dysfunction (ED) in men is a worldwide problem. Histopathological changes in diabetic corpora cavernosa (CC) tissue are not fully understood due to limited studies on human tissues. The aim of this study was to compare histopathological changes between CC of diabetic and nondiabetic patients with ED. Patients and methods Forty CC biopsies were taken from 16 diabetic and 24 nondiabetic men during penile implant insertion. Formalin-fixed paraffin-embedded sections from each specimen were stained with conventional hematoxylin and eosin stains for general assessment of histopathological changes, with Masson trichrome for assessment of smooth muscle (SM) fibers and collagen, and with Verhoeff's elastic van Gieson stain for assessment of elastic fibers (EF). SM fibers were assessed for cell thickness/bundle, shape, and percentage from trabecular tissue thickness. EF were assessed for shape and percentage from trabecular tissue. Arteries and venous sinuses were assessed for presence of subendothelial fibrosis. The main outcome measures were thickness and shape of trabecular SM bundles, percentage of trabecular EF and shape, and presence of subendothelial fibrosis. ResultsNo difference in SM thickness was detected; however, SM in the diabetic group showed higher degree of shape abnormality in the form of frayed fibers (P = 0.007) and more intramuscular fibrosis (P = 0.001). The percentage of collagen fibers was significantly higher in diabetic patients (P = 0.002). The percentage of EF in trabecular tissue ranged from 10 to 60% and was significantly reduced in diabetic patients (P = 0.015). Subendothelial fibrosis within arteries and sinuses was higher in the diabetic group (P = 0.059 and 0.002, respectively). ConclusionThe major structural changes within CC tissue in diabetic patients were fraying and fibrosis in SM, increased trabecular collagen fibers, and reduction of EF. These changes may play a role in prevention of SM relaxation and interference with proper sinus filling, and therefore ED.

show abstract

Section: Discussionsupporting

confidence: 90%

“…In experimental studies, it was found that diabetic animals showed reduction in penile vascular tissue compared with controls [27]. In experimental studies, it was found that diabetic animals showed reduction in penile vascular tissue compared with controls [27].…”

Section: Discussionmentioning

confidence: 99%

Morphological assessment of corpora cavernosa tissue in diabetic and nondiabetic patients with erectile dysfunction

Mahran¹,

Mohammed²,

Radwan³

et al. 2014

Human Andrology

View full text Add to dashboard Cite

show abstract

“…Previous studies that involved micro-CT imaging of the vasculature predominantly have used either barium sulfatebased contrast agents (27,31,44) or polymerizing compounds containing lead chromate (7,12,19,23,33,38,43). As shown in our preliminary studies, choice of the ideal contrast agent can be a strong determinant of image quality for some applications.…”

Section: Discussionmentioning

confidence: 78%

“…Recently, vascular imaging procedures have been developed for small animal models based on the use of perfused contrast agents and X-ray micro-CT (7,12,19,23,27,33,38,43,47). The goal of these studies has been to visualize 3-D vascular networks in organs (i.e., heart and kidneys) or tumors.…”

mentioning

confidence: 99%

Quantitative microcomputed tomography analysis of collateral vessel development after ischemic injury

Duvall¹,

Taylor²,

Weiss³

et al. 2004

American Journal of Physiology-Heart and Circulatory Physiology

211

218

View full text Add to dashboard Cite

Transgenic mouse models are increasingly being used to investigate the functions of specific growth factors or matrix proteins to design therapeutic strategies for controlling blood vessel growth. However, the available methodologies for evaluating angiogenesis and arteriogenesis in these models are limited by animal size, user subjectivity, the power to visualize the three-dimensional vessel networks, or the capability to employ a vigorous quantitative analysis. In this study, we employed contrast-enhanced microcomputed tomography imaging to assess collateral development after induction of hindlimb ischemia in the mouse. The morphological parameters vessel volume, connectivity, number, thickness, thickness distribution, separation, and degree of anisotropy were evaluated in control and surgery limbs 0, 3, and 14 days postsurgery. Results indicate that the vascular volume of the surgically manipulated limb was reconstituted as early as 3 days after femoral artery excision through development of a series of highly connected, small caliber, closely spaced, and isotropically oriented collateral vessels. Parametric analyses were completed to assess the sensitivity of the calculated morphological parameters to variations in image binarization threshold and voxel size. Images taken at the 36-microm voxel size were found to be optimal for evaluating collateral vessel formation, whereas 8- to 16-microm voxel sizes were needed to resolve smaller vascular structures. This study demonstrates the utility of microcomputed tomography as a robust method for quantitative, three-dimensional analysis of blood vessel networks. Whereas these initial efforts focused on the mouse hindlimb ischemia model, the developed techniques may be applied to a variety of model systems to investigate mechanisms of angiogenesis and arteriogenesis.

show abstract

“…One example would be Simopoulos et al's work on diabetes. 134 Through quantitative micro CT analysis, a significant drop in corporeal vascular volume and luminal area were observed with on set of diabetes. Ritman and co-workers have centered their work on renal vasculature and its response to kidney failure.…”

Section: Micro Ct Imaging: Vasculature Studiesmentioning

confidence: 99%

The Biomedical Applications of Computed Tomography

Tuan

Hutmacher

2007

Biomechanical Systems Technology

View full text Add to dashboard Cite

Computed Tomography (CT) imaging is playing an increasingly important role in the biomedical sciences of today as clinicians and scientists begin to recognize its potential in health care and research. Clinical CT facilitates the early diagnosis of diseases thus allowing the selection of appropriate therapies which improves the patient's overcome. Pre-surgical imaging aids operation planning while real time scans assist complicated high risk surgeries. One of the primary concerns in clinical CT procedures would be the adverse radiation side effects thus only low dosages are allowed. The resolution of clinical imaging is insufficient for research, hence the micro CT set up has to be used. The advent of micro CT imaging has ushered in the possibility of non destructive quantitative analysis for trabeculae architectures, scaffolds, soft tissues and biological constructs. This paper highlights the inherent potential and emerging biomedical applications of CT. 193 Biomechanical Systems Technology Downloaded from www.worldscientific.com by UNIVERSITY OF QUEENSLAND on 08/15/15. For personal use only.

show abstract

Corporeal Structural and Vascular Micro Architecture With X-Ray Micro Computerized Tomography in Normal and Diabetic Rabbits: Histopathological Correlation

Cited by 36 publications

References 42 publications

Morphological assessment of corpora cavernosa tissue in diabetic and nondiabetic patients with erectile dysfunction

Morphological assessment of corpora cavernosa tissue in diabetic and nondiabetic patients with erectile dysfunction

Quantitative microcomputed tomography analysis of collateral vessel development after ischemic injury

The Biomedical Applications of Computed Tomography

Contact Info

Product

Resources

About