Coronin 1B supports RhoA signaling at cell-cell junctions through Myosin II

Priya, Rashmi; Wee, Kenneth; Budnar, Srikanth; Gómez, Guillermo A.; Yap, Alpha S.; Michael, Magdalene

doi:10.1080/15384101.2016.1234549

Cited by 12 publications

(13 citation statements)

References 45 publications

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“…The heterogeneous changes of actin structures around the extrusion site were found to be coupled with the non-uniform remodeling of CCJ between the extruding cell and its neighbors. Previous studies showed that the reduction of E-cadherin at the junctions, together with a reduction of tension at the lateral membrane, occurs before extrusion [6,11,36]. The reduced tension at the CCJs is necessary for increased contractility that expels the cell [11],…”

Section: A Theoretical Model To Reconcile Forces Produced By Purse-stmentioning

confidence: 99%

Adhesion-mediated heterogeneous actin organization governs apoptotic cell extrusion

Rupprecht

Mège

et al. 2020

Preprint

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Apoptotic extrusion is crucial in maintaining epithelial homeostasis and has implications in diseases of epithelial tissues. Current literature supports that epithelia respond to extrusion to maintain their integrity by the formation of a supracellular actomyosin ring (purse-string) in the neighbors that encompasses the dying cells. However, little is known about whether other types of actin structures could contribute to extrusion as well as how forces generated by mechanosensitive proteins in the cells are integrated. Here, we found that during extrusion, a heterogeneous actin network composed of lamellipodia protrusions and discontinuous actomyosin cables, was reorganized in the neighboring cells and was the main factor driving extrusion forwards. The early presence of basal lamellipodia protrusion participated both in basal sealing of the extrusion site and in orienting the actomyosin purse-string at the later stage of extrusion. These sequential events are essential in ensuring a successful extrusion in apicobasal direction. The co-existence of these two mechanisms is determined by the interplay between the cell-cell and cell-substrate adhesions. A theoretical model integrates the role of these cellular mechanosensitive components to explain why a dual-mode mechanism, which combined lamellipodia protrusion and purse-string contractility, leads to more efficient extrusion than a single-mode mechanism. We anticipate that our approach will be useful to provide mechanistic insight into epithelial homeostasis, morphogenetic events and tumorigenesis.

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Section: A Theoretical Model To Reconcile Forces Produced By Purse-stmentioning

confidence: 99%

Adhesion-mediated heterogeneous actin organization governs apoptotic cell extrusion

Rupprecht

Mège

et al. 2020

Preprint

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“…Recent work has positioned Coronin 1B as the critical actin-binding protein for restructuring perijunctional actin from a branched F-actin network to contractile bundles (Michael et al, 2016). Furthermore, Coronin is required for stabilizing Myosin II at the AJC, which can positively regulate Rho signaling (Michael et al, 2016; Priya et al, 2016). …”

Section: Actin-binding Proteins At Cell-cell Junctions: Organizing F-mentioning

confidence: 99%

Rho GTPases and actomyosin: Partners in regulating epithelial cell-cell junction structure and function

Arnold

Stephenson

Miller

2017

Experimental Cell Research

121

118

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Epithelial tissues are defined by polarized epithelial cells that are integrated into tissues and exhibit barrier function in order to regulate what is allowed to pass between cells. Cell-cell junctions must be stable enough to promote barrier function and tissue integrity, yet plastic enough to remodel when necessary. This remarkable ability to dynamically sense and respond to changes in cell shape and tissue tension allows cell-cell junctions to remain functional during events that disrupt epithelial homeostasis including morphogenesis, wound healing, and cell division. In order to achieve this plasticity, both tight junctions and adherens junctions are coupled to the underlying actomyosin cytoskeleton. Here, we discuss the importance of the junctional linkage to actomyosin and how a localized zone of active RhoA along with other Rho GTPases work together to orchestrate junctional actomyosin dynamics. We focus on how scaffold proteins help coordinate Rho GTPases, their upstream regulators, and their downstream effectors for efficient, localized Rho GTPase signaling output. Additionally, we highlight important roles junctional actin-binding proteins play in addition to their traditional roles in organizing actin. Together, Rho GTPases, their regulators, and effectors form compartmentalized signaling modules that regulate actomyosin structure and contractility to achieve proper cell-cell adhesion and tissue barriers.

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“…Further, ROCK was recently found to participate in a feedback network that supported RhoA signaling at cadherin junctions (Priya et al. , 2015, 2016). However, distinct contributions of ROCK1 and ROCK2 to junctional biology have yet to be investigated in detail.…”

Section: Introductionmentioning

confidence: 99%

ROCK1 but not ROCK2 contributes to RhoA signaling and NMIIA-mediated contractility at the epithelial zonula adherens

Priya

Liang

Teo

et al. 2017

MBoC

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Coronin 1B supports RhoA signaling at cell-cell junctions through Myosin II

Cited by 12 publications

References 45 publications

Adhesion-mediated heterogeneous actin organization governs apoptotic cell extrusion

Adhesion-mediated heterogeneous actin organization governs apoptotic cell extrusion

Rho GTPases and actomyosin: Partners in regulating epithelial cell-cell junction structure and function

ROCK1 but not ROCK2 contributes to RhoA signaling and NMIIA-mediated contractility at the epithelial zonula adherens

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