Coronatine Alleviates Water Deficiency Stress on Winter Wheat Seedlings

Abstract

With the aim to determine whether coronatine (COR) alleviates drought stress on wheat, two winter wheat (Triticum aestivum L.) cultivars, ChangWu134 (drought-tolerant) and Shan253 (drought-sensitive) were studied under hydroponic conditions. Seedlings at the three-leaf stage were cultured in a Hoagland solution containing COR at 0.1 microM for 24 h, and then exposed to 20% polyethylene glycol 6000 (PEG-6000). Under simulated drought (SD), COR increased the dry weight of shoots and roots of the two cultivars significantly; the root/shoot ratio also increased by 30% for Shan253 and 40% for ChangWu134. Both cultivars treated with COR under SD (0.1COR+PEG) maintained significantly higher relative water content, photosynthesis, transpiration, intercellular concentration of CO(2) and stomatal conductance in leaves than those not treated with PEG. Under drought, COR significantly decreased the relative conductivity and malondialdehyde production, and the loss of 1,1-diphenyl-2-picrylhydrazyl scavenging activity in leaves was significantly alleviated in COR-treated plants. The activity of peroxidase, catalase, glutathione reductase and ascorbate peroxidase were adversely affected by drought. Leaves of plants treated with COR under drought produced less abscisic acid (ABA) than those not treated. Thus, COR might alleviate drought effects on wheat by reducing active oxygen species production, activating antioxidant enzymes and changing the ABA level.

Impact of osmotic stress on seedling growth observations, membrane characteristics and antioxidant defense system of different wheat genotypes

“…Also, severe drought has more intensive effect than moderate drought (Table 3). These results agree with those of Li et al [28] who observed significant increase in membrane lipid peroxidation of different wheat cultivars grown under water deficit. As a similar response, MDA Fig.…”

“…GR was also inhibited in all the surveyed genotypes except in moderately-droughted Gimmaza 9 and 11 as well as stressed Sids 13, Sakha 94 and Giza 186. Reduction in CAT and GR activity in water-unsatisfied wheat was previously noted [28]. Suppressed activity of CAT and GR under stress may result from the reverse effect of stress on protein biosynthesis.…”

Growth and physiological responses to water and nutrient stress in oil palm

“…As shown in Table 2, the results show that RWC and Chlorophyll a/b decreased with the water stress in both fertilizer supply and without fertilizer supply conditions, while the parameter, leaf relative conductivity decreased under the fertilizer supply only under well-watered conditions but greatly increased under water-stressed conditions. Similar results were also reported by previous studies (Legros et al, 2009;Song et al, 2010;Sanchez et al, 1983;Correia et al, 1989;Marulanda et al, 2010;Li et al, 2010;Yousfi et al, 2010). When the treatment was observed up to 60 or 90 days, our result showed that RWC and Chlorophyll a/b was significantly lower and leaf relative conductivity was significantly higher in water-stressed treatment than in the well-watered treatment, but it increased with nutrient supply.…”

Combining Physiological and Metabolomic Analysis to Unravel the Regulations of Coronatine Alleviating Water Stress in Tobacco (Nicotiana tabacum L.)

“…Additionally, GR is a vital constituent of the AsA-GSH cycle and takes part in ROS detoxification via maintaining GSH in its reduced state [37]. Consistent with the results of previous studies of COR-induced stress tolerance in plants [38,39], our results indicated that the positive role of COR-mediated tobacco seedlings to relieve oxidative injury involves improvement in antioxidant enzyme activities.…”