Corona-induced micro-centrifugal flows for concentration of Neisseria and Salmonella bacteria prior to their quantitation using antibody-functionalized SERS-reporter nanobeads

Chen, Yuan Yu; Fang, Yu Cheng; Lin, Shih Yun; Lin, Yi Jyun; Yen, Shih Ying; Yang, Chiou-Ying; Chau, Lai-Kwan; Wang, Shau Chun

doi:10.1007/s00604-017-2077-7

Cited by 15 publications

(9 citation statements)

References 29 publications

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“…Another report showed that ionic wind near a corona needle tip above a small liquid reservoir can generate microcentrifugal flows and trap suspended bacteria within a few minutes. Bacteria decorated with antibody-functionalized SERS micro-tags were concentrated and enriched at the reservoir bottom and can then be recognized and quantified by SERS signals from the tags [ 132 ].…”

Section: Microfluidics-sersmentioning

confidence: 99%

Selectivity/Specificity Improvement Strategies in Surface-Enhanced Raman Spectroscopy Analysis

Wang¹,

Cao²,

Yan³

et al. 2017

Sensors

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Surface-enhanced Raman spectroscopy (SERS) is a powerful technique for the discrimination, identification, and potential quantification of certain compounds/organisms. However, its real application is challenging due to the multiple interference from the complicated detection matrix. Therefore, selective/specific detection is crucial for the real application of SERS technique. We summarize in this review five selective/specific detection techniques (chemical reaction, antibody, aptamer, molecularly imprinted polymers and microfluidics), which can be applied for the rapid and reliable selective/specific detection when coupled with SERS technique.

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Section: Microfluidics-sersmentioning

confidence: 99%

Selectivity/Specificity Improvement Strategies in Surface-Enhanced Raman Spectroscopy Analysis

Wang¹,

Cao²,

Yan³

et al. 2017

Sensors

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“…Such a device should integrate the ability for bacteria concentration particularly. Currently used set‐ups mostly utilize pressure‐assisted filtration [7,14,15], discharge‐induced flow [16,17], and solvent evaporation [18] for this purpose. Unfortunately, all of these techniques require very sophisticated technologies or external apparatuses hardly compatible with a portable device.…”

Section: Introductionmentioning

confidence: 99%

Microfluidic device for concentration and SERS‐based detection of bacteria in drinking water

et al. 2020

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There is a constant need for the development of easy-to-operate systems for the rapid and unambiguous identification of bacterial pathogens in drinking water without the requirement for time-consuming culture processes. In this study, we present a disposable and lowcost lab-on-a-chip device utilizing a nanoporous membrane, which connects two stacked perpendicular microfluidic channels. Whereas one of the channels supplies the sample, the second one attracts it by potential-driven forces. Surface-enhanced Raman spectrometry (SERS) is employed as a reliable detection method for bacteria identification. To gain the effect of surface enhancement, silver nanoparticles were added to the sample. The pores of the membrane act as a filter trapping the bodies of microorganisms as well as clusters of nanoparticles creating suitable conditions for sensitive SERS detection. Therein, we focused on the construction and characterization of the device performance. To demonstrate the functionality of the microfluidic chip, we analyzed common pathogens (Escherichia coli DH5α and Pseudomonas taiwanensis VLB120) from spiked tap water using the optimized experimental parameters. The obtained results confirmed our system to be promising for the construction of a disposable optical platform for reliable and rapid pathogen detection which couples their electrokinetic concentration on the integrated nanoporous membrane with SERS detection.

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“…Our group has successfully developed electrohydrodynamic micro-concentrators to trap a small sample aliquot (30–50 µL) containing pathogenic bacteria including Neisseria and Salmonella using centrifugal flows generated by corona discharge-induced wind [ 24 , 25 ]. By hanging a needle electrode with a high AC voltage (≥1 kV) of 50 kHz about 3 mm above the surface of a sample droplet on an electrically grounded slide, we can create large amounts of plasma ions at the needle tip to collide with electroneutral air molecules, generating the airflow known as ionic wind.…”

Section: Introductionmentioning

confidence: 99%

“…These NAEBs have been demonstrated to provide a ultrahigh-sensitive SERS signal from even a single NAEB, due to the Raman molecule-induced “hot-spots” in the nanoaggregates. [ 26 ] Raman signals from the bound tags on bacteria can be employed to quantify pathogen cell numbers even down to the single bacterium level [ 18 , 24 , 25 , 27 , 28 ]. When a sample droplet of 30 μL was evaporated in half an hour using ionic wind to concentrate the collected bacteria, detection of a few cells of Salmonella was successfully accomplished.…”

Section: Introductionmentioning

confidence: 99%

Integration of a Thermoelectric Heating Unit with Ionic Wind-Induced Droplet Centrifugation Chip to Develop Miniaturized Concentration Device for Rapid Determination of Salmonella on Food Samples Using Antibody-Functionalized SERS Tags

Chen

Wang

et al. 2020

Sensors

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When a centrifugation-enriched sample of 100 μL containing the surface-enhanced Raman scattering (SERS) tag-bound bacteria (Salmonella in this study) is siphoned onto a glass slide next to an embedded thermoelectric heating chip, such a sessile droplet is quickly evaporated. As the size of the sample droplet is significantly reduced during the heating process, ionic wind streams from a corona discharge needle, stationed above the sample, sweep across the liquid surface to produce centrifugal vortex flow. Tag-bound Salmonella in the sample are then dragged and trapped at the center of droplet bottom. Finally, when the sample is dried, unlike the “coffee ring” effect, the SERS tag-bound Salmonella is concentrated in one small spot to allow sensitive detection of a Raman signal. Compared with our previous electrohydrodynamic concentration device containing only a corona discharge needle, this thermoelectric evaporation-assisted device is more time-effective, with the time of concentrating and drying about 100 μL sample reduced from 2 h to 30 min. Hence, sample throughput can be accelerated with this device for practical use. It is also more sensitive, with SERS detection of a few cells of Salmonella in neat samples achievable. We also evaluated the feasibility of using this device to detect Salmonella in food samples without performing the culturing procedures. Having spiked a few Salmonella cells into ice cubes and lettuce leaves, we use filtration and ultracentrifugation steps to obtain enriched tag-bound Salmonella samples of 200 μL. After loading an aliquot of 100 μL of sample onto this concentration device, the SERS tag signals from samples of 100 g ice cubes containing two Salmonella cells and 20 g lettuce leaf containing 5 Salmonella cells can be successfully detected.

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Corona-induced micro-centrifugal flows for concentration of Neisseria and Salmonella bacteria prior to their quantitation using antibody-functionalized SERS-reporter nanobeads

Cited by 15 publications

References 29 publications

Selectivity/Specificity Improvement Strategies in Surface-Enhanced Raman Spectroscopy Analysis

Selectivity/Specificity Improvement Strategies in Surface-Enhanced Raman Spectroscopy Analysis

Microfluidic device for concentration and SERS‐based detection of bacteria in drinking water

Integration of a Thermoelectric Heating Unit with Ionic Wind-Induced Droplet Centrifugation Chip to Develop Miniaturized Concentration Device for Rapid Determination of Salmonella on Food Samples Using Antibody-Functionalized SERS Tags

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