Corona discharge plasma stimulated production of atropine in callus of Datura inoxia by DNA hypomethylation and gene regulation: a novel technology for plant cell and tissue culture

Seed priming with corona discharge plasma modified growth performance, improved metabolism, and elicited production of tropane alkaloids in Datura inoxia seedlings; plasma technology for application in plant in-vitro cultures

“…Catalase and peroxidase are integral components of an enzymatic antioxidant system, thereby protecting the plant cell against oxidative stress. The advantages of cold plasma recorded in Datura seedlings are in agreement with the ndings of Darigh et al, [21] in Cannabis, Jiang et al, [22] in tomato, Pérez-Pizá et al, [23] in Glycine max, Tardast et al, [10] in Datura inoxia, Zhang et al, [24] in soybean. The non-thermal plasma treatment was capable of increasing stem diameter, plant height, biomass of roots, and yield in soybean seedlings [23].…”

“…For instance, exposure of soybean seeds to plasma was associated with modi cation in the transcription levels of energy metabolism-related genes and several growthregulating factors (GRFs), which play vital roles in controlling plant growth and development [24]. Taking the recorded increase in tropane alkaloids into account, the application of cold plasma accelerated the accumulation of secondary metabolites in different plant species, such as, Stevia rabaudiana [17], Oryza sativa [25], cannabis [21], Datura inoxia [10], red clover [26], and Fenugreek [27].…”

“…This plasma-forming device capable of generating stable non-thermal plasma at atmospheric pressure consists of two parts, including (i) an AC power supply and (ii) 2 copper wire electrodes with distance of 1.5 cm placed inside a Te on tube. We provide a schematic image of plasma-generating device in our recently published article [10] in which we report the effect of plasma on Datura callogenesis performance. The experimental conditions during the plasma formation included (i) voltage: 15 kV, (ii) Frequency: 40 kHz, and (iii) power: 40 W. After placing the copper electrodes on the top and bottom of the Petri dishes containing germinating seeds the air inside the container turns into the plasma which was validated by the optical emission spectroscopy (Ocean Optics HR 2000, Germany) under the wavelengths ranging from 200 to 1000 nm and identi cation of peaks based on NIST Atomic Spectra Database [10].…”

“…It has been well-documented that plant species belonging to the Datura genus possess a variety of secondary metabolites, such as alkaloids, terpenoids, and avonoids, which have valuable medicinal and therapeutic applications [9,10]. For this reason, various strategies, such as applications of chemical [11], physical [10], and biotic elicitors [12] have been employed to stimulate the production of secondary metabolites of this plant under laboratory cultivation conditions. Despite the development of seed disinfection procedures, endogenic seed-borne contaminations restrict the e ciency of tissue or organ cultures of medicinal plants.…”

“…Plasma treatment has been investigated for its potential molecular effects on plants, particularly in terms of gene expression [14,10], epigenetic regulation [10,15], stress resistance [15], pathogen inactivation ([16], and metabolic pathways [17]. Literature data suggest that the plasma treatment can promote defense mechanisms and enhance plant growth, development, and metabolism [18].…”