Cornus officinalis Ethanolic Extract with Potential Anti-Allergic, Anti-Inflammatory, and Antioxidant Activities

Abstract

Atopic dermatitis (AD) is an allergic and chronic inflammatory skin disease. The present study investigates the anti-allergic, antioxidant, and anti-inflammatory activities of the ethanolic extract of Cornus officinalis (COFE) for possible applications in the treatment of AD. COFE inhibits the release of β-hexosaminidase from RBL-2H3 cells sensitized with the dinitrophenyl-immunoglobulin E (IgE-DNP) antibody after stimulation with dinitrophenyl-human serum albumin (DNP-HSA) in a concentration-dependent manner (IC50 = 0.178 mg/mL). Antioxidant activity determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, ferric reducing antioxidant power assay, and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) scavenging activity, result in EC50 values of 1.82, 10.76, and 0.6 mg/mL, respectively. Moreover, the extract significantly inhibits lipopolysaccharide (LPS)-induced nitric oxide (NO) production and the mRNA expression of iNOS and pro-inflammatory cytokines (IL-1β, IL-6, and TNF-α) through attenuation of NF-κB activation in RAW 264.7 cells. COFE significantly inhibits TNF-α-induced apoptosis in HaCaT cells without cytotoxic effects (p < 0.05). Furthermore, 2-furancarboxaldehyde and loganin are identified by gas chromatography/mass spectrometry (GC-MS) and liquid chromatography with tandem mass spectrometry (LC-MS/MS) analysis, respectively, as the major compounds. Molecular docking analysis shows that loganin, cornuside, and naringenin 7-O-β-D-glucoside could potentially disrupt the binding of IgE to human high-affinity IgE receptors (FceRI). Our results suggest that COFE might possess potential inhibitory effects on allergic responses, oxidative stress, and inflammatory responses.

Potential Application of Cornelian Cherry Extract on Broiler Chickens: Growth, Expression of Antioxidant Biomarker and Glucose Transport Genes, and Oxidative Stability of Frozen Meat

“…Among these natural compounds is cornelian cherry ( Cornus mas ) extract (CCE), which is composed of several active compounds, including five anthocyanins: delphinidin 3-galactoside, cyanidin 3-rhamnosylgalactoside, cyanidin 3-galactoside, pelargonidin 3-rhamnosylgalactoside, and pelargonidin 3-galactoside [ 12 ]. Ursolic acid is an important constituent of CCE [ 13 , 14 , 15 , 16 ] possessing antioxidant and antibacterial properties. Additionally, it contains vitamin C, trace minerals, organic acids, pectintriterpenoids, iridoids, pectins, and tannins that range within the safe standards levels of food [ 17 , 18 , 19 ].…”

Antiosteoarthritic Effect of Morroniside in Chondrocyte Inflammation and Destabilization of Medial Meniscus-Induced Mouse Model

“…Manu et al have indicated the beneficial effects of CO on type 2 diabetes, neuroinflammation, and bone loss [ 21 , 22 , 23 , 24 ]. Recent studies have shown that the CO extract possesses potent anti-inflammatory action against oxidative stress in RAW 264.7 cells and decreases proinflammatory factors, such as IL-1β, interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-α), in numerous types of inflammation [ 25 , 26 , 27 ]. Additionally, aqueous extracts of the CO fruit reportedly inhibit OA symptoms in a testosterone-deficient mouse model [ 28 ].…”

Ameliorative Effects of Loganin on Arthritis in Chondrocytes and Destabilization of the Medial Meniscus-Induced Animal Model

“…In addition, pharmaceutical bioactive components derived from CO extract have been identified, including loganin, morroniside, caffeic acid, and ursolic acid [ 22 ]. Loganin, one of the most abundant compounds in CO extract, has been reported to be an anti-inflammatory agent by reducing reactive oxygen species and cytokines [ 23 , 24 ] and inhibiting inflammatory responses by attenuating NF-κB downstream signaling [ 25 , 26 ].…”