Corneal Ulcers Associated With Contact Lens Wear

Galentine, Paul; Cohen, Elisabeth J.; Laibson, Peter R.; Adams, Charles P.; Michaud, Rollande; Arentsen, Juan J.

doi:10.1001/archopht.1984.01040030711025

Cited by 148 publications

(54 citation statements)

References 21 publications

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“…Pseudomonas aeruginosa was also found to be a major causative organism in previous studies too [24][25][26][27][28][29]. It was found that Pseudomonas aeruginosa have a tendency to adhere to the contact lens surface and it penetrates the cornea's deeper layers and leading corneal ulcers, which could cause permanent blindness [23].…”

Section: Discussionsupporting

confidence: 41%

Bacterial Keratitis Risk Factors, Pathogens and Antibiotic Susceptibilities: A 5- Year Review of Cases at Dubai hospital, Dubai

Elhanan¹,

Nabi²

2016

J Clin Exp Ophthalmol

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Objective: Microbial keratitis is a sight threatening infection of the cornea. Its incidence has been increased in the past few years, with the contact lens wear as the major risk factor. In the past few years other risk factors have also come up in light. We thus aimed to present a 5-year study comprising of 37 patients with microbial keratitis who yielded only positive culture; other cases with negative cultures were excluded.Methods: Local microbiology database and retrospective audit of patients (who had a corneal scraping for culture over a 5-year period) medical records were used in this study. Results:We found that in our study also contact lens wear is the major risk factor for microbial keratitis. Pseudomonas aeruginosa was the most widely recognized causative organism isolated, present in 37% of the patient's cultures. We found an association between risk factors for keratitis and variables collectively using multivariate analysis (p value<0.001), and an association of age with the risk factor for keratitis on performing separate ANOVA for each variable (p value<0.001). Conclusion:This study will help the clinical management of patients with keratitis and will raise awareness of sufficient lens care and disinfection practices.

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Section: Discussionsupporting

confidence: 41%

Bacterial Keratitis Risk Factors, Pathogens and Antibiotic Susceptibilities: A 5- Year Review of Cases at Dubai hospital, Dubai

Elhanan¹,

Nabi²

2016

J Clin Exp Ophthalmol

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“…The eye is a notable tissue to evaluate such effects, not only due to its basal immune-privileged status but also because inflammatory-mediated damage to tissues like the cornea that result in clearance of infecting microbes also leads to significant damage (35) that severely compromises eyesight, whereas comparable scarring in other tissues is often of little consequence. Pseudomonas aeruginosa is a leading cause of bacterial eye infections in humans (2,13,29,33), and interventions are needed that promote bacterial clearance while limiting tissue-associated pathology due to a rapid and extensive influx of neutrophils.Neutrophil recruitment to infected tissues, particularly tissues infected with extracellular bacteria and fungi (7, 25), is often highly dependent on the proinflammatory cytokine interleukin-17 (IL-17) produced by helper T cells called Th17 cells (11,21), although the importance of IL-17 varies by pathogen, tissue, and type of infection. For example, antibody-mediated depletion of IL-17 or deficiency of the IL-17 receptor (IL-17R) had no effect on the course of P. aeruginosa lung infection, but these interventions did diminish the protective efficacy of a live-attenuated P. aeruginosa vaccine against lethal pneumonia (27).…”

mentioning

confidence: 43%

mentioning

confidence: 43%

Topical Neutralization of Interleukin-17 during Experimental Pseudomonas aeruginosa Corneal Infection Promotes Bacterial Clearance and Reduces Pathology

2012

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ABSTRACTThe proinflammatory cytokine interleukin-17 (IL-17) is involved in neutrophilic tissue infiltration, contributing to both microbial clearance as well as inflammation-associated tissue damage. Its role during bacterial corneal infections is unknown. We hypothesized that IL-17 responses would be detrimental in this setting and tested the impact of IL-17 receptor deficiency or antibody-mediated neutralization of IL-17 in a murine model ofPseudomonas aeruginosaulcerative keratitis after scratch injury. We found that, compared with infected corneas from wild-type mice, those from IL-17 receptor (IL-17R)-deficient mice had significantly lower corneal pathology scores, neutrophil influx, and intracellular bacterial levels. Infected IL-17R-deficient corneas had low intercellular adhesion molecule 1 (ICAM-1) expression, and ICAM-1-deficient mice were similarly resistant to infection. Topical treatment with polyclonal antibodies to IL-17 resulted in significant reductions in corneal pathology and also lowered bacterial counts after infection with six different laboratory or clinicalP. aeruginosastrains, including both invasive and cytotoxic strains. Thus, neutralization of IL-17 duringP. aeruginosacorneal infection reduces neutrophil influx and pathology without compromising bacterial clearance and offers a promising new avenue for therapy of these potentially sight-threatening infections.

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“…Pseudomonas aeruginosa is the pathogen most frequently isolated in cases of contact lens-associated bacterial keratitis (1,10,38,41), a rapidly progressive infection that can lead to permanent scarring of the cornea and loss of vision. Even when antibiotic treatment eradicates the offending pathogen, corneal damage from inflammation can still occur (24).…”

mentioning

confidence: 45%

A Live-Attenuated Pseudomonas aeruginosa Vaccine Elicits Outer Membrane Protein-Specific Active and Passive Protection against Corneal Infection

2006

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Pseudomonas aeruginosa can cause sight-threatening corneal infections in humans, particularly those who wear contact lenses. We have previously shown that a live-attenuated P. aeruginosa vaccine given intranasally protected mice against acute lethal pneumonia in a lipopolysaccharide (LPS) serogroup-specific manner. In the current study, we evaluated the protective and therapeutic efficacies, as well as the target antigens, of this vaccine in a murine corneal infection model. C3H/HeN mice were nasally immunized with the vaccine (an aroA deletion mutant of strain PAO1, designated PAO1⌬aroA) or with Escherichia coli as a control and were challenged 3 weeks later by inoculating the scratch-injured cornea with P. aeruginosa. For passive prophylaxis and therapy, we utilized a serum raised in rabbits nasally immunized with PAO1⌬aroA or E. coli. Outcome measures included corneal pathology scores and, in some experiments, reductions in total and internalized bacterial CFU. We found that both active and passive immunization reduced corneal pathology scores after challenge with a variety of P. aeruginosa strains, including several serogroup-heterologous strains. Even when given therapeutically starting as late as 24 h after infection, the rabbit antiserum to PAO1⌬aroA was effective at reducing corneal pathology scores. Immunotherapy of established infections also reduced the numbers of total and internalized corneal P. aeruginosa bacteria. Experiments using absorbed sera showed that the protective antibodies are specific to outer membrane proteins. Thus, live-attenuated P. aeruginosa vaccines delivered nasally protect against corneal infections in mice and potentially can be used to prepare passive therapy reagents for the treatment of established P. aeruginosa corneal infections caused by diverse LPS serogroups.

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Corneal Ulcers Associated With Contact Lens Wear

Cited by 148 publications

References 21 publications

Bacterial Keratitis Risk Factors, Pathogens and Antibiotic Susceptibilities: A 5- Year Review of Cases at Dubai hospital, Dubai

Bacterial Keratitis Risk Factors, Pathogens and Antibiotic Susceptibilities: A 5- Year Review of Cases at Dubai hospital, Dubai

Topical Neutralization of Interleukin-17 during Experimental Pseudomonas aeruginosa Corneal Infection Promotes Bacterial Clearance and Reduces Pathology

A Live-Attenuated Pseudomonas aeruginosa Vaccine Elicits Outer Membrane Protein-Specific Active and Passive Protection against Corneal Infection

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