Corneal stromal abscess in a cat

Moore, David L.; Jones, R Gareth

doi:10.1111/j.1748-5827.1994.tb03875.x

Cited by 3 publications

(1 citation statement)

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“…Disease conditions affecting any or all parts of the cornea can result in permanent changes in clarity that greatly impair vision and would be best treated with a corneal transplant. Many ophthalmic conditions exist for corneal transplantation across species, including endothelial dystrophy/degeneration, 1 –3 stromal abscess, 4 –6 chronic superficial keratitis, 7 deep corneal ulcers or descemetoceles, 8 squamous cell carcinoma, 9 and globe enlarging procedures for intraocular prosthesis placement 8…”

Section: Introductionmentioning

confidence: 99%

Comparison of Optisol‐GS and neomycin‐polymyxin B‐gramicidin ophthalmic solution for corneal storage in the dog

Andrew

Samuelson

Lewis

et al. 1999

Veterinary Ophthalmology

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The objective of the research was to compare the efficacy of Optisol-GS (OGS, Bausch & Lomb Surgical, Irvine, CA, USA) with triple antibiotic ophthalmic solution (neomycin-polymyxin B-gramicidin, NPG; Bausch & Lomb, Tampa, FL, USA) in preserving the viability of corneal endothelial cells. The study subjects were thirty young to middle-aged dogs with no gross corneal pathology that had been euthanized by pentobarbital overdose for reasons unrelated to this project. Corneal tissues were harvested, analyzed, and randomly assigned to treatment groups: one of two media (OGS or NPG), and one of five storage times (1, 7, 14, 21, or 35 days). Six corneas were stored in each medium for each time period. Corneal endothelial cell viability was evaluated pre- and poststorage by vital staining (trypan blue and alizarin red S), and endothelial cell morphology was evaluated with scanning electron microscopy. Storage in NPG caused significant loss (100%) of endothelial cells after all storage times. OGS storage maintained a high level of endothelial cell viability up to 21 days (98.9% +/- 1.3% viability). A significant decrease in percentage viability was also found for OGS-stored corneas between 21 and 35 days, when endothelial cell viability decreased to 61.4% +/- 45.9%. The conclusions are that NPG storage at -20 degrees C is a very poor choice of media for corneal tissue banking if graft clarity is the goal. Storage in Optisol-GS at 4 degrees C for up to 21 days resulted in significantly higher percentages of viable endothelial cells. Optisol-GS storage should facilitate corneal preservation for canine keratoplasty patients.

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Section: Introductionmentioning

confidence: 99%