Corneal Resistance to Keratolysis After Collagen Crosslinking With Rose Bengal and Green Light

Fadlallah, Ali; Zhu, Hong; Arafat, Samer N.; Kochevar, Irene E.; Melki, Samir; Ciolino, Joseph B.

doi:10.1167/iovs.15-18764

Cited by 20 publications

(9 citation statements)

References 25 publications

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“…8 Another determining factor of success is penetration of rose bengal into the cornea. Rose bengal, like other photosensitizers, has limited diffusion into the cornea (~100–150um).…”

Section: Discussionmentioning

confidence: 99%

Rose Bengal Photodynamic Antimicrobial Therapy: A Novel Treatment for Resistant Fusarium Keratitis

Amescua

Arboleda

Nikpoor

et al. 2017

Cornea

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Background Fungal keratitis is a commonly occurring cause of corneal blindness which does not always respond to standard antifungal treatment. Rose bengal photodynamic antimicrobial therapy represents an alternative treatment that has worked in vitro and that was successfully implemented in the clinic to treat an aggressive infectious keratitis case. Purpose To evaluate the efficacy of rose bengal photodynamic antimicrobial therapy (PDAT) for the management of a patient with multidrug-resistant Fusarium keratoplasticum keratitis unresponsive to standard clinical treatment. Results In vitro results demonstrated the efficacy of rose bengal PDAT against a multidrug-resistant Fusarium keratoplasticum species. There was complete fungal inhibition in our irradiation zone on the agar plates. In the clinical case, the patient was successfully treated with two sessions of rose bengal PDAT, and at the eight month follow-up, there was no recurrence of infection nor adverse effects to report. Conclusions and Relevance Rose bengal PDAT is a novel treatment that may be considered in cases of aggressive infectious keratitis. Further studies are needed to understand the mechanisms of PDAT in-vivo.

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“…8 Another determining factor of success is penetration of rose bengal into the cornea. Rose bengal, like other photosensitizers, has limited diffusion into the cornea (~100–150um).…”

Section: Discussionmentioning

confidence: 99%

Rose Bengal Photodynamic Antimicrobial Therapy: A Novel Treatment for Resistant Fusarium Keratitis

Amescua

Arboleda

Nikpoor

et al. 2017

Cornea

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“…The safety of the treatment was demonstrated through histological analyses showing no thermal damage to retina and iris cells of rabbits after PTB in vivo. 28 Over recent years, more studies on the comparison between collagen crosslinking induced by photo-activated riboflavin and RB on ex vivo and in vivo efficacy, 29 corneal healing response, 30 and collagenase degradation 31 have been performed, showing that treatment with photo-activated RB is more effective than with riboflavin. Based on these results, photo-activated RB to crosslink corneal tissue is considered an attractive therapeutic approach for keratoconus and other disorders and its clinical application in humans should be explored in the near future.…”

Section: Photo-and Sono-activated Rose Bengalmentioning

confidence: 99%

Biomedical Applications of Photo- and Sono-Activated Rose Bengal: A Review

Vanerio

Stijnen

Mol³

et al. 2019

Photobiomodulation, Photomedicine, and Laser Surgery

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DOI to the publisher's website. • The final author version and the galley proof are versions of the publication after peer review. • The final published version features the final layout of the paper including the volume, issue and page numbers. Link to publication General rights Copyright and moral rights for the publications made accessible in the public portal are retained by the authors and/or other copyright owners and it is a condition of accessing publications that users recognise and abide by the legal requirements associated with these rights. • Users may download and print one copy of any publication from the public portal for the purpose of private study or research. • You may not further distribute the material or use it for any profit-making activity or commercial gain • You may freely distribute the URL identifying the publication in the public portal. If the publication is distributed under the terms of Article 25fa of the Dutch Copyright Act, indicated by the "Taverne" license above, please follow below link for the End User Agreement:

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“…Although the time lag for full digestion of untreated control samples in our study is in line with previous publications, direct comparison should consider differences in treatment protocols and available information regarding study parameters-in particular the enzymatic activity of the applied assay. 12,21,22 Therefore we chose to directly compare WST/NIR and RF/UVA CXL. All four formulations showed a very strong reduced rate of enzymatic digestion after treatment, indicating the keratolytic resistance of both RF/UVA and WST/NIR derived corneal stiffening.…”

Section: Discussionmentioning

confidence: 99%

Enzymatic Digestion of Porcine Corneas Cross-linked by Hypo- and Hyperosmolar Formulations of Riboflavin/ultraviolet A or WST11/Near-Infrared Light

Brekelmans

Veugen

Rieff

et al. 2020

Trans. Vis. Sci. Tech.

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To assess enzymatic digestion rate after Riboflavin (RF) and Water-Soluble-Taurine (WST11) based corneal cross-linking (CXL), with or without the addition of high molecular weight dextran (RF-D and WST-D). Methods: Eighty-eight paired porcine corneas were cross-linked by either RF (n = 11) or RF-D (n = 11) and ultraviolet light (UVA), or WST11 (n = 11) or WST-D (n = 11) and nearinfrared (NIR) light, or used as paired control (n = 44). Corneal buttons of treated and paired control eyes were placed in a 0.3% collagenase solution. Time to full digestion and remaining dry sample weight after six hours were compared. Results: A strong treatment effect was seen with all four formulations, as all controls had been fully digested whilst all treated samples were still visible at the experiment's endpoint. After irradiation, central corneal thickness was significantly higher in samples treated with hypo-osmolar formulations, compared to dextran enriched formulations (P < 0.001). Dry sample weight after digestion was nonsignificantly different between corneas treated by the four different formulations (P = 0.102). Average dry sample weight was 1.68 ± 0.6 (n = 10), 2.19 ± 0.50 (n = 8), 1.48 ± 0.76 (n = 11), and 1.54 ± 0.60 (n = 9) mg, for RF, RF-D, WST11, and WST-D treated samples, respectively. Enzymatic resistance was similar for RF and WST based CXL (P = 0.61) and was not affected by the addition of dextran (P = 0.221). Conclusions: Both RF and WST11 based CXL significantly increases resistance to enzymatic digestion, with similar effect for hypo-osmolar and hyperosmolar (dextran enriched) formulations. Translational Relevance: Our findings indicate these formulations are interchangeable, paving the way for the development of novel PACK-CXL protocols for thin corneas and deep-seated infections.

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Corneal Resistance to Keratolysis After Collagen Crosslinking With Rose Bengal and Green Light

Abstract: Crosslinking with RGX increased corneal resistance to digestion by collagenase comparable to that produced by riboflavin and UVA treatment.

Cited by 20 publications

References 25 publications

Rose Bengal Photodynamic Antimicrobial Therapy: A Novel Treatment for Resistant Fusarium Keratitis

Rose Bengal Photodynamic Antimicrobial Therapy: A Novel Treatment for Resistant Fusarium Keratitis

Biomedical Applications of Photo- and Sono-Activated Rose Bengal: A Review

Enzymatic Digestion of Porcine Corneas Cross-linked by Hypo- and Hyperosmolar Formulations of Riboflavin/ultraviolet A or WST11/Near-Infrared Light

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