Corneal regeneration by transplantation of corneal epithelial cell sheets fabricated with automated cell culture system in rabbit model

Kobayashi, Toyoshige; Kan, Kazutoshi; Nishida, Kohji; Yamato, Masayuki; Okano, Teruo

doi:10.1016/j.biomaterials.2013.07.065

Cited by 48 publications

(27 citation statements)

References 29 publications

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“…Osteogenic differentiation assay of the cell sheet showed that high-level osteogenic differentiation capacity was retained. In future, we will use ontogenetically induced cells to form cell sheets, as is often performed during myocardial or corneal tissue engineering [38, 39]. …”

Section: Discussionmentioning

confidence: 99%

Local delivery of HMGB1 in gelatin sponge scaffolds combined with mesenchymal stem cell sheets to accelerate fracture healing

et al. 2017

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Fracture nonunion and delayed union continue to pose challenges for orthopedic surgeons. In the present study, we combined HMGB1 gelatin sponges with MSC sheets to promote bone healing after surgical treatment of rat tibial fractures. The HMGB1 gelatin sponge scaffolds supported the expansion of mesenchymal stem cells (MSCs) and promoted the osteogenic differentiation of MSCs and MSC sheets. Lentiviral vectors were then used to overexpress HMGB1 in MSCs. The results indicated that HMGB1 promotes the osteogenic differentiation of MSCs through the STAT3 pathway. Both siRNA and a STAT3 inhibitor downregulated STAT3, further confirming that HMGB1 induces the osteogenic differentiation of MSCs partly via the STAT3 signal pathway. In a rat tibial osteotomy model, we demonstrated the ability of HMGB1 gelatin sponge scaffolds to increase bone formation. The addition of MSC sheets further enhanced fracture healing. These findings support the use of HMGB1-loaded gelatin sponge scaffolds combined with MSC sheets to enhance fracture healing after surgical intervention.

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Section: Discussionmentioning

confidence: 99%

Local delivery of HMGB1 in gelatin sponge scaffolds combined with mesenchymal stem cell sheets to accelerate fracture healing

et al. 2017

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“…13 In vitro the corneal epithelial layer has been successfully generated by cell sheet engineering, and it has been shown to promote in vitro growth of a functional stratified epithelium. [28][29][30] In addition, engineered epithelial tissue sheets, prepared using thermoresponsive polymeric substrates, have been clinically evaluated using autologous oral mucosal epithelium ( Fig. 2A).…”

Section: Corneal Epitheliummentioning

confidence: 99%

Corneal Tissue Engineering: Recent Advances and Future Perspectives

Ghezzi

Rnjak‐Kovacina

Kaplan

2015

Tissue Engineering Part B: Reviews

164

151

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“…Unlike conventional methods that require an enzymatic reaction (Kushida et al , , ; Yamato and Okano, ), this method of automation has significantly increased productivity, and minimized the number of procedural steps and risk of contamination during the handling of cell sheets. This technology has since progressed into the pre‐clinical models for use in the repair of various tissues, such as cornea, skin, oesophagus, heart and periodontal tissue (Iwata et al , ; Kobayashi et al , ; Nishida et al , ; Sawa et al , ; Takagi et al , ).…”

Section: Sensing and Automationmentioning

confidence: 99%

Review: bioreactor design towards generation of relevant engineered tissues: focus on clinical translation

Ravichandran

Liu

Teoh

2017

J Tissue Eng Regen Med

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In tissue engineering and regenerative medicine, studies that utilize 3D scaffolds for generating voluminous tissues are mostly confined in the realm of in vitro research and preclinical animal model testing. Bioreactors offer an excellent platform to grow and develop 3D tissues by providing conditions that mimic their native microenvironment. Aligning the bioreactor development process with a focus on patient care will aid in the faster translation of the bioreactor technology to clinics. In this review, we discuss the various factors involved in the design of clinically relevant bioreactors in relation to their respective applications. We explore the functional relevance of tissue grafts generated by bioreactors that have been designed to provide physiologically relevant mechanical cues on the growing tissue. The review discusses the recent trends in non-invasive sensing of the bioreactor culture conditions. It provides an insight to the current technological advancements that enable in situ, non-invasive, qualitative and quantitative evaluation of the tissue grafts grown in a bioreactor system. We summarize the emerging trends in commercial bioreactor design followed by a short discussion on the aspects that hamper the 'push' of bioreactor systems into the commercial market as well as 'pull' factors for stakeholders to embrace and adopt widespread utility of bioreactors in the clinical setting. Copyright © 2017 John Wiley & Sons, Ltd.

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Corneal regeneration by transplantation of corneal epithelial cell sheets fabricated with automated cell culture system in rabbit model

Cited by 48 publications

References 29 publications

Local delivery of HMGB1 in gelatin sponge scaffolds combined with mesenchymal stem cell sheets to accelerate fracture healing

Local delivery of HMGB1 in gelatin sponge scaffolds combined with mesenchymal stem cell sheets to accelerate fracture healing

Corneal Tissue Engineering: Recent Advances and Future Perspectives

Review: bioreactor design towards generation of relevant engineered tissues: focus on clinical translation

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