Corneal neovascularization in rats as a model for photothrombotic therapy using bacteriochlorin a and an argon laser

Gool, C. A. M. van; Schuitmaker, Hans J.; Jager, Martine J.

doi:10.1007/bf00180948

Cited by 7 publications

(1 citation statement)

References 22 publications

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“…Several methods to induce experimental corneal neovascularization have been established [2,26,27]. The chemical cauterization model has the advantages of technical simplicity and reproducibility.…”

Section: Discussionmentioning

confidence: 99%

Expression of Matrix Metalloproteinases 2 and 9 and Tissue Inhibitors of Metalloproteinase 1 and 2 in Inflammation-Induced Corneal Neovascularization

Hui‐Kang

Chen²,

Kim³

et al. 2001

Ophthalmic Res

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Purpose: Matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinase (TIMPs) have been linked to the angiogenic process in general. In order to understand the potential roles of MMP-2, MMP-9 and TIMPs in the corneal neovascularization process, we examined the expression and activities of MMP-2, MMP-9 and TIMPs during the course of cauterization-induced corneal neovascularization in a rat model. Methods: Neovascularization of rat corneas was induced by silver nitrate cauterization. The expression of MMP-2, MMP-9, TIMP-1 and TIMP-2 was examined by immunohistochemistry and RT-PCR. The protein activities of MMPs and TIMPs were compared in pre- and postcauterization corneas by gelatin zymography and reverse zymography, respectively. Results: MMP-2, TIMP-1 and TIMP-2 immunoreactivities were expressed in normal corneas, predominantly in the corneal epithelium. After injury, immunoreactivities of both MMPs and TIMPs were increased, notably in the healing corneal epithelium, infiltrating inflammatory cells, stromal fibroblasts and ingrowing vascular endothelial cells. The increase in gross MMP-2 enzymatic activity paralleled the maximal vascular ingrowth on day 4, while the gross MMP-9 enzymatic activity rose immediately on day 1, then decreased steadily, which paralleled the magnitude of inflammatory cell infiltration. The immunoreactivity of MMPs/TIMPs decreased significantly 2 weeks after cauterization. On day 35, MMP-2, TIMP-1 and TIMP-2 staining was seen only in corneal epithelium and vascular endothelial cells. Both the RT-PCR and reverse zymography results revealed a more constant expression of TIMP-2, while the TIMP-1 expression appeared to be more inducible. Conclusion: MMPs as well as TIMPs were upregulated in cauterization-induced corneal neovascularization, suggesting that both may participate in extracellular matrix remodeling in the corneal wound healing, inflammation and neovascularization processes.

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Section: Discussionmentioning

confidence: 99%

Expression of Matrix Metalloproteinases 2 and 9 and Tissue Inhibitors of Metalloproteinase 1 and 2 in Inflammation-Induced Corneal Neovascularization

Hui‐Kang

Chen²,

Kim³

et al. 2001

Ophthalmic Res

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Wissenschaftliche Aspekte der innovativen Lasermedizin

Litscher¹

2015

Laserakupunktur Und Innovative Lasermedizin

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Inhibition of Ocular Angiogenesis by Diced Small Interfering RNAs (siRNAs) Specific to Vascular Endothelial Growth Factor (VEGF)

Murata

Takanami

Shimizu

et al. 2006

Current Eye Research

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The effects of diced small interfering RNAs (siRNAs) designed for vascular endothelial growth factor (VEGF) on the expression of VEGF in human retinal pigment epithelial cell line ARPE-19 cells in vitro and on corneal angiogenesis in vivo were examined. The exposure to diced siRNAs significantly reduced the VEGF mRNA expression in ARPE-19 cells with minimal toxicity. In suture-induced corneal angiogenesis models, diced siRNAs minimized the severity of angiogenesis. Histological analysis displayed no particular tissue damage in the conjunctiva where siRNA was injected. The approach using diced siRNAs can be a new tool for various neovascular ocular diseases.

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Corneal neovascularization in rats as a model for photothrombotic therapy using bacteriochlorin a and an argon laser

Abstract: Suture-induced corneal neovascularization in the rat can be used as a model to study the efficacy of photothrombosis therapy. BCA can be used to enhance the rate and duration of vessel closure.

Cited by 7 publications

References 22 publications

Expression of Matrix Metalloproteinases 2 and 9 and Tissue Inhibitors of Metalloproteinase 1 and 2 in Inflammation-Induced Corneal Neovascularization

Expression of Matrix Metalloproteinases 2 and 9 and Tissue Inhibitors of Metalloproteinase 1 and 2 in Inflammation-Induced Corneal Neovascularization

Wissenschaftliche Aspekte der innovativen Lasermedizin

Inhibition of Ocular Angiogenesis by Diced Small Interfering RNAs (siRNAs) Specific to Vascular Endothelial Growth Factor (VEGF)

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