Corneal haze after photorefractive keratectomy using different epithelial removal techniques11The authors have no proprietary interest in any of the equipment mentioned in this article.

Lee, Young G; Chen, Willie Y.W; Petroll, Walter M; Cavanagh, Harrison D; Jester, James V.

doi:10.1016/s0161-6420(00)00426-7

Cited by 45 publications

(9 citation statements)

References 34 publications

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“…and LASIK are shown in Table 1. The mean measured Consistent with the current data, Lee et al, 6 using ablation depth after PRK was not significantly different tandem scanning confocal microscopy, found no differfrom the predicted ablation depth (1.1 Ϯ 11.1 m, ence between the measured and intended ablation P ϭ .84). The mean measured ablation depth 1 month depths after PRK.…”

Section: Resultssupporting

confidence: 88%

Confocal microscopy evaluation of stromal ablation depth after myopic laser in situ keratomileusis and photorefractive keratectomy

Erie

Hodge

Bourne

2004

Journal of Cataract and Refractive Surgery

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Section: Resultssupporting

confidence: 88%

Confocal microscopy evaluation of stromal ablation depth after myopic laser in situ keratomileusis and photorefractive keratectomy

Erie

Hodge

Bourne

2004

Journal of Cataract and Refractive Surgery

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“…Lee et al 9 . evaluated corneal healing after PRK using mechanical and laser scrap techniques and reported that there is no significant difference in the corneal wound healing response between these two techniques.…”

Section: Discussionmentioning

confidence: 99%

“…There are several methods for epithelial debridement including mechanical, chemical, rotating brush, and using Excimer laser. Previous studies demonstrated that all of these epithelial removal techniques are effective for surgical correction of refractive errors 5, 6, 7, 8, 9. Mechanical debridement seems to be the most common technique for epithelial debridement.…”

Section: Introductionmentioning

confidence: 99%

Comparison of mechanical debridement and trans-epithelial myopic photorefractive keratectomy: A contralateral eye study

Zarei‐Ghanavati

Shandiz

Abrishami

et al. 2019

Journal of Current Ophthalmology

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Purpose To compare clinical outcomes between mechanical debridement photorefractive keratectomy (m-PRK) and trans-epithelial photorefractive keratectomy (t-PRK) in myopic patients. Methods Eighty eyes of 40 myopic patients with age between 18 and 55 years were included in this study. In each patient, one eye was randomly assigned for t-PRK, using the Amaris laser's ORK-CAM software and the other eye for m-PRK, using a spatula. Stromal ablation was done by Schwind Amaris 750S. Uncorrected and best corrected visual acuity (BCVA), refractive outcomes, epithelial healing, pain, and discomfort were compared between the groups on day 1, 3, 7 and month 1, 3, and 6. Results Preoperative spherical equivalent (SE) were −3.97 ± 2.08 diopter (D) and −3.98 ± 2.06 D in m-PRK and t-PRK eyes, respectively ( P = 0.981). Operation time was significantly shorter in the t-PRK group than m-PRK ( P < 0.001). Postoperative pain was experienced significantly higher in the t-PRK group measured by 11-point numeric scale of pain questionnaire on the first postoperative day ( P < 0.001). Photophobia, tearing, and vision fluctuation were also significantly higher in the t-PRK group postoperatively. However epithelial defect size and re-epithelialization time were lower in the t-PRK group ( P = 0.012 and P < 0.001, respectively). Postoperative parameters including SE, uncorrected distance visual acuity (UDVA), corrected distance visual acuity (CDVA), and contrast acuity did not show any significant difference between the two groups during all intervals. Conclusions Although epithelial defect size and epithelial healing time were lower in t-PRK, postoperative pain, photophobia, and vision fluctuation were significantly less in the m-PRK group in the first postoperative days. There was no statistically significant difference between the groups after one week, and both mechanical and trans-epithelial techniques were shown to be safe and effective.

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“…Fibroblastic cells invading the wound area transform into myofibroblasts that remove and synthesize new ECM, express alpha smooth muscle actin, and exert strong contractile forces [16][17][18]. The replacement of stromal keratocytes by fibrotic tissue produced by myofibroblasts alters corneal transparency, resulting in corneal haze and vision impairment [19,20]. TGFβ1 plays a central role in myofibroblast transformation of corneal keratocytes during wound healing [21][22][23].…”

Section: Introductionmentioning

confidence: 99%

Coupling of Fibrin Reorganization and Fibronectin Patterning by Corneal Fibroblasts in Response to PDGF BB and TGFβ1

Miron-Mendoza¹,

Vazquez²,

García-Rámila³

et al. 2020

Bioengineering

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We previously reported that corneal fibroblasts within 3D fibrin matrices secrete, bind, and organize fibronectin into tracks that facilitate cell spreading and migration. Other cells use these fibronectin tracks as conduits, which leads to the development of an interconnected cell/fibronectin network. In this study, we investigate how cell-induced reorganization of fibrin correlates with fibronectin track formation in response to two growth factors present during wound healing: PDGF BB, which stimulates cell spreading and migration; and TGFβ1, which stimulates cellular contraction and myofibroblast transformation. Both PDGF BB and TGFβ1 stimulated global fibrin matrix contraction (p < 0.005); however, the cell and matrix patterning were different. We found that, during PDGF BB-induced cell spreading, fibronectin was organized simultaneously with the generation of tractional forces at the leading edge of pseudopodia. Over time this led to the formation of an interconnected network consisting of cells, fibronectin and compacted fibrin tracks. Following culture in TGFβ1, cells were less motile, produced significant local fibrin reorganization, and formed fewer cellular connections as compared to PDGF BB (p < 0.005). Although bands of compacted fibrin tracks developed in between neighboring cells, fibronectin labeling was not generally present along these tracks, and the correlation between fibrin and fibronectin labeling was significantly less than that observed in PDGF BB (p < 0.001). Taken together, our results show that cell-induced extracellular matrix (ECM) reorganization can occur independently from fibronectin patterning. Nonetheless, both events seem to be coordinated, as corneal fibroblasts in PDGF BB secrete and organize fibronectin as they preferentially spread along compacted fibrin tracks between cells, producing an interconnected network in which cells, fibronectin and compacted fibrin tracks are highly correlated. This mechanism of patterning could contribute to the formation of organized cellular networks that have been observed following corneal injury and refractive surgery.

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Corneal haze after photorefractive keratectomy using different epithelial removal techniques11The authors have no proprietary interest in any of the equipment mentioned in this article.

Cited by 45 publications

References 34 publications

Confocal microscopy evaluation of stromal ablation depth after myopic laser in situ keratomileusis and photorefractive keratectomy

Confocal microscopy evaluation of stromal ablation depth after myopic laser in situ keratomileusis and photorefractive keratectomy

Comparison of mechanical debridement and trans-epithelial myopic photorefractive keratectomy: A contralateral eye study

Coupling of Fibrin Reorganization and Fibronectin Patterning by Corneal Fibroblasts in Response to PDGF BB and TGFβ1

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