Corneal Endothelium: Isolation and Cultivation Methods

Mingo-Botín, David; Balgos, Marie Joan Therese D; Arnalich‐Montiel, Francisco

doi:10.1007/978-3-030-01304-2_28

Cited by 2 publications

(2 citation statements)

References 82 publications

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“…The resulting membrane was found to be transparent, biodegradable, and suitable for CEpC attachment and proliferation. As noted above AM also showed potential as a mechanical support of transplanted limbal stem cells (LSCs) [37], limbal epithelial stem cells (LESCs) [58], corneal endothelial cells (CEnCs) [59], CSC cells [60], and CEC cells [61].…”

Section: Membrane-and Film-based Graftsmentioning

confidence: 67%

Tissue Engineering Meets Nanotechnology: Molecular Mechanism Modulations in Cornea Regeneration

et al. 2021

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Nowadays, tissue engineering is one of the most promising approaches for the regeneration of various tissues and organs, including the cornea. However, the inability of biomaterial scaffolds to successfully integrate into the environment of surrounding tissues is one of the main challenges that sufficiently limits the restoration of damaged corneal tissues. Thus, the modulation of molecular and cellular mechanisms is important and necessary for successful graft integration and long-term survival. The dynamics of molecular interactions affecting the site of injury will determine the corneal transplantation efficacy and the post-surgery clinical outcome. The interactions between biomaterial surfaces, cells and their microenvironment can regulate cell behavior and alter their physiology and signaling pathways. Nanotechnology is an advantageous tool for the current understanding, coordination, and directed regulation of molecular cell–transplant interactions on behalf of the healing of corneal wounds. Therefore, the use of various nanotechnological strategies will provide new solutions to the problem of corneal allograft rejection, by modulating and regulating host–graft interaction dynamics towards proper integration and long-term functionality of the transplant.

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Section: Membrane-and Film-based Graftsmentioning

confidence: 67%

Tissue Engineering Meets Nanotechnology: Molecular Mechanism Modulations in Cornea Regeneration

et al. 2021

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“…Baum et al (1979) described these cells in 1977 as cells with potency to proliferate in vitro in the presence of appropriate stimuli. The cadaver corneas, which lack the quality for transplantation and donated corneoscleral rims remaining after transplantation, are the most common source for isolation of primary HCECs for application in cell therapy and tissue engineering approaches (Mingo-Botín et al, 2019). Generally, in vitro-expanded HCECs are isolated from the donated corneas that are pooled and induced to force proliferation.…”

Section: Hcecsmentioning

confidence: 99%

Corneal endothelium tissue engineering: An evolution of signaling molecules, cells, and scaffolds toward 3D bioprinting and cell sheets

Khalili

Asadi

Kahroba

et al. 2020

Journal Cellular Physiology

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Cornea is an avascular and transparent tissue that focuses light on retina. Cornea is supported by the corneal‐endothelial layer through regulation of hydration homeostasis. Restoring vision in patients afflicted with corneal endothelium dysfunction‐mediated blindness most often requires corneal transplantation (CT), which faces considerable constrictions due to donor limitations. An emerging alternative to CT is corneal endothelium tissue engineering (CETE), which involves utilizing scaffold‐based methods and scaffold‐free strategies. The innovative scaffold‐free method is cell sheet engineering, which typically generates cell layers surrounded by an intact extracellular matrix, exhibiting tunable release from the stimuli‐responsive surface. In some studies, scaffold‐based or scaffold‐free technologies have been reported to achieve promising outcomes. However, yet some issues exist in translating CETE from bench to clinical practice. In this review, we compare different corneal endothelium regeneration methods and elaborate on the application of multiple cell types (stem cells, corneal endothelial cells, and endothelial precursors), signaling molecules (growth factors, cytokines, chemical compounds, and small RNAs), and natural and synthetic scaffolds for CETE. Furthermore, we discuss the importance of three‐dimensional bioprinting strategies and simulation of Descemet's membrane by biomimetic topography. Finally, we dissected the recent advances, applications, and prospects of cell sheet engineering for CETE.

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Corneal Endothelium: Isolation and Cultivation Methods

Cited by 2 publications

References 82 publications

Tissue Engineering Meets Nanotechnology: Molecular Mechanism Modulations in Cornea Regeneration

Tissue Engineering Meets Nanotechnology: Molecular Mechanism Modulations in Cornea Regeneration

Corneal endothelium tissue engineering: An evolution of signaling molecules, cells, and scaffolds toward 3D bioprinting and cell sheets

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