Corneal Endothelium in Herpetic Keratouveitis

Vannas, Antti; Ahonen, R.; Mäkitie, Jukka

doi:10.1001/archopht.1983.01040010913011

Cited by 44 publications

(13 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Recurrent disease can lead to scarring of the cornea, visual impairment and eventually blindness. The disease has been shown to affect corneal epithelial cells (Coleman et al, 1969), keratocytes (Kaufman, 1975) and endothelial cells (Vannas et al, 1983).…”

Section: Introductionmentioning

confidence: 43%

Herpes Simplex Virus Type 1 Latency in Rabbit Corneal Cells in Vitro: Reactivation and Recombination Following Intratypic Superinfection of Long Term Cultures

1987

Journal of General Virology

View full text Add to dashboard Cite

SUMMARYHerpes simplex virus type 1 (HSV-1) has been isolated from explanted human corneas after cultivation in vitro. To determine whether HSV-1 is persistent or latent in corneal cells, a system to study HSV-1 infection of rabbit corneal cells in vitro was developed. By elevation of the incubation temperature to 42 °C before and during HSV-1 infection it was shown that both keratocytes and epithelial cells support a nonproductive rather than a productive infection. On subsequent temperature reduction to 37 °C, infectious virus was released from both cell types. Addition of the viral inhibitor acycloguanosine during the last 5 days of a 14 day incubation at 42 °C did not reduce the frequency of viral shedding following transfer to 37 °C, indicating that corneal cells support a latent as opposed to persistent infection. Cultures which failed to shed virus spontaneously up to 29 days post-inoculation were superinfected at 37 °C, with an XbaI site deletion mutant of HSV-1. Restriction endonuclease analysis of progeny identified both the initial infecting virus and recombinants between the parental and superinfecting genomes.

show abstract

Section: Introductionmentioning

confidence: 43%

Herpes Simplex Virus Type 1 Latency in Rabbit Corneal Cells in Vitro: Reactivation and Recombination Following Intratypic Superinfection of Long Term Cultures

1987

Journal of General Virology

View full text Add to dashboard Cite

show abstract

“…In some cases, less deep vascularization of varying severity was present. Occasionally, evidence of inflammation seemingly localized to the region of Descemet's membrane was found [2,17,18]. Most patients were adults, and the typical case was unilateral and characterized by an acute onset with symptoms of irritation, pain, photophobia, lacrimation, and circumcorneal injection.…”

Section: Introductionsupporting

confidence: 41%

Sectorial keratitis and uveitis: differential diagnosis

Baltatzis

Romero-Rangel

Maleki

2003

Graefe's Arch Clin Exp Ophthalmol

View full text Add to dashboard Cite

show abstract

“…However, they could not rule out the lesions as nonspe cific signs of endothelial damage and regen eration, since these lesions could be produced when either bovine serum albumin or sodium hydroxide was applied to the cornea. As an HSV-specific sign of endothelial damage, loss of endothelial cells was observed after injec tion of HSV into the anterior chamber or herpetic keratouveitis [ 11,12], In the herpet ic stromal keratitis model, Metcalf and Rei chert [8] demonstrated attack of PMN against rabbit endothelium.…”

Section: Discussionmentioning

confidence: 47%

Participation of T Lymphocyte in Corneal Edema in the Early Stage of Herpetic Stromal Keratitis

et al. 1988

View full text Add to dashboard Cite

The participation of T lymphocytes in the immunopathogenesis of corneal opacity in herpetic stromal keratitis was investigated. In BALB/c mice infected intracorneally with herpes simplex virus type 1 corneal opacity was manifested 10 days after infection, while in athymic mice corneas remained almost clear. Histologically, all opaque corneas revealed stromal edema accompanied by the diffuse presence of polymorphonuclear cells and lymphocytes. When complement (C’)-treated immune spleen cells were adoptively transferred into athymic mice 6 or 72 h after corneal infection, stromal keratitis with mild opacity was observed 10 days after transfer. The athymic mice given anti-Thy 1.2 + C’-treated immune spleen cells failed to develop corneal opacity. The difference, as revealed by light and electron microscopy, was the presence or absence of lymphocytic infiltration and edema in the posterior third layers of the stroma and endothelial lesions. The endothelium was infiltrated by lymphocytes or macrophages and showed various stages of destruction. The main cause of corneal opacity in the early stage of herpetic stromal keratitis is thought to be stromal edema due to an adverse effect on the endothelium by immune T lymphocytes.

show abstract

Corneal Endothelium in Herpetic Keratouveitis

Cited by 44 publications

References 13 publications

Herpes Simplex Virus Type 1 Latency in Rabbit Corneal Cells in Vitro: Reactivation and Recombination Following Intratypic Superinfection of Long Term Cultures

Herpes Simplex Virus Type 1 Latency in Rabbit Corneal Cells in Vitro: Reactivation and Recombination Following Intratypic Superinfection of Long Term Cultures

Sectorial keratitis and uveitis: differential diagnosis

Participation of T Lymphocyte in Corneal Edema in the Early Stage of Herpetic Stromal Keratitis

Contact Info

Product

Resources

About