Corneal Edema After Descemet Membrane Stripping Automated Endothelial Keratoplasty With the Use of Gentian Violet Staining

Ray, Vincent L. G.; Cronin, Kevin M.; Walter, Keith A.

doi:10.1001/archophthalmol.2011.2493

Cited by 8 publications

(7 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Vincent et al reported two cases that underwent DSAEK with donor grafts marked with GV. 14 In the first postoperative week post-DSAEK, both cases had marked corneal edema in the area of the GV markings and bullae. 14 However, unlike our case, the edema gradually resolved over weeks, and the vision improved to 20/40.…”

Section: Discussionmentioning

confidence: 93%

“… 14 In the first postoperative week post-DSAEK, both cases had marked corneal edema in the area of the GV markings and bullae. 14 However, unlike our case, the edema gradually resolved over weeks, and the vision improved to 20/40. 14 In a recently published paper, two cases of persistent localized DMEK detachments secondary to GV ink have been reported.…”

Section: Discussionmentioning

confidence: 93%

See 1 more Smart Citation

Gentian violet (GV) ink associated reaction in a case of preloaded Descemet membrane endothelial keratoplasty: Case report

Alosaimy,

Bin Helayel,

Ahad

2024

American Journal of Ophthalmology Case Reports

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 93%

Gentian violet (GV) ink associated reaction in a case of preloaded Descemet membrane endothelial keratoplasty: Case report

Alosaimy,

Bin Helayel,

Ahad

2024

American Journal of Ophthalmology Case Reports

View full text Add to dashboard Cite

“…These methods include “double ring” sign, pre-placement of a hitch suture, “acute angled-beveled” sign or pre-marking with gentian violet may. [ 6 7 8 9 10 11 12 ] However, loss of orientation can occur prior to insertion that can be due to prior separation of the lenticule from its cap or while tackling incomplete separation, eccentric trephination or flipped graft before insertion. [ 15 ] The double-ring sign can clear the ambiguity to a certain extent at the end of surgery; but it may not always be discernable as its appearance is governed by the thickness and discrepancy between the anterior and posterior curvature of the lenticule.…”

Section: Discussionmentioning

confidence: 99%

“…[ 5 ] Techniques like double-ring sign aid in identifying lenticule orientation after insertion into anterior chamber (AC). [ 6 7 8 9 10 11 12 ] However, loss of orientation can occur even before its insertion into AC, commonly with ultra-thin lenticules and novice-surgeons. [ 13 14 15 ] Though rare, this could compel the surgeon to replace the tissue, which is undesirable given the preexisting dearth of donor corneas.…”

mentioning

confidence: 99%

Dye-based identification of the orientation of tissue for Descemet stripping automated endothelial keratoplasty: A laboratory-based study

Donthineni

Vaddavalli

2021

Indian Journal of Ophthalmology

View full text Add to dashboard Cite

Purpose: To describe the features enabling the identification of the orientation of Descemet stripping automated endothelial keratoplasty (DSAEK) lenticule with the assistance of vital dyes. Methods: This is a blinded experimental lab-based study, including 30 microkeratome prepared precut DSAEK lenticules. The lenticules were divided into control and study arms which included 10 unstained and 20 stained lenticules, respectively. In the study arm, vital dyes like trypan blue (TB), brilliant blue (BB), indocyanine green (ICG) and fluorescein stain (FS) were used to stain 5 lenticules each. They were examined by experienced (group 1) and novice surgeons (group 2) to identify the correct orientation of the lenticule. The results were tabulated and analyzed. Results: Of the 30 lenticules examined, the average of total scores obtained by each observer was higher (78%) in group 1 as compared to group 2 (65.3%) which was statistically significant ( P < 0.005). In group 1, the accuracy of identifying the correct orientation of unstained lenticules was 70% which improved to 82% on staining. The accuracy in group 2 was 58% with unstained lenticules which improved to 69% on staining. Within the study arm, irrespective of surgical experience, the accuracy was highest with BB (86%), followed by TB (82%), ICG (72%) and FS (62%). Conclusion: This study found that the accuracy of identifying the orientation of DSAEK lenticules increased with experience and with the assistance of staining using vital dyes. This accuracy improved with blue dyes like brilliant blue and trypan blue, irrespective of the level of experience.

show abstract

“…Gentian violet (GV) can be used to mark DSAEK donor stroma to facilitate appropriate tissue orientation in the anterior chamber. However, markings can persist at the graft- host interface [52], and there are case reports of GV causing significant corneal edema after DSAEK [53]. Using an in vitro model, Ide et al demonstrated that GV markings on the donor stroma damage the corneal endothelium [54].…”

Section: Introductionmentioning

confidence: 99%

Eye-bank preparation of endothelial tissue

Boynton

Woodward

2014

Current Opinion in Ophthalmology

View full text Add to dashboard Cite

Purpose of review Eyebank preparation of endothelial tissue for keratoplasty continues to evolve. While eye bank personnel have become comfortable and competent at Descemet Stripping Automated Endothelial Keratoplasty (DSAEK) tissue preparation and tissue transport, optimization of preparation methods continues. Surgeons and eye bank personnel should be up to date on the research in the field. As surgeons transition to Descemet Membrane Endothelial Keratoplasty (DMEK), eye banks have risen to the challenge of preparing tissue. Eye banks are refining their DMEK preparation and transport techniques Recent findings This article covers refinements to DSAEK tissue preparation, innovations to prepare DMEK tissue, and nuances to improve donor cornea tissue quality. Summary As eye bank supplied corneal tissue is the main source of tissue for many corneal surgeons, it is critical to stay informed about tissue handling and preparation. Ultimately the surgeon is responsible for the transplantation, so involvement of clinicians in eye banking practices and advocacy for pursuing meaningful research in this area will benefit clinical patient outcomes.

show abstract

Corneal Edema After Descemet Membrane Stripping Automated Endothelial Keratoplasty With the Use of Gentian Violet Staining

Cited by 8 publications

References 5 publications

Gentian violet (GV) ink associated reaction in a case of preloaded Descemet membrane endothelial keratoplasty: Case report

Gentian violet (GV) ink associated reaction in a case of preloaded Descemet membrane endothelial keratoplasty: Case report

Dye-based identification of the orientation of tissue for Descemet stripping automated endothelial keratoplasty: A laboratory-based study

Eye-bank preparation of endothelial tissue

Contact Info

Product

Resources

About