The "4R" approach of using the right rate, right source, right timing, and right placement is an accepted framework for increasing crop N use efficiency. However, modifying only one 4R component does not consistently reduce nitrous oxide (N 2 O) emissions. Our objective was to determine if N fertilizer applied in three split applications (Sp), by itself or combined with changes in N source and rate, could improve N recovery efficiency (NRE) and N surplus (NS) and decrease N 2 O emissions. Over two corn (Zea mays L.) growing seasons in Minnesota, N 2 O emissions ranged from 0.6 to 0.9 kg N ha -1. None of the treatment combinations affected grain yield. Compared with urea applied in a single application at the recommended N rate, Sp by itself did not improve NRE or NS and did not decrease N 2 O. Combining Sp with urease and nitrification inhibitors and/or a 15% reduction in N rate increased NRE from 57 to >73% and decreased NS by >20 kg N ha . The only treatment that decreased N 2 O (by 20-53%) was Sp combined with inhibitors and reduced N rate. Emissions of N 2 O were more strongly correlated with NS calculated from grain N uptake (R 2 = 0.61) compared with whole-plant N uptake (r 2 = 0.39), possibly because most N losses occurred before grain filling. Optimizing both application timing and N source can allow for a moderate reduction in N rate that does not affect grain yield but decreases N 2 O. Grain-based NS may be a more useful indicator of N 2 O emissions than whole-plant-based NS.
Evaluation of Intensive "4R" Strategies for Decreasing Nitrous Oxide Emissions and Nitrogen Surplus in Rainfed CornRodney T. Venterea,* Jeffrey A. Coulter, and Michael S. Dolan O ptimizing the four basic aspects of N fertilizer management-the "4R" approach of using the right rate, right source, right timing, and right placement-is often recommended for increasing crop N use efficiency and decreasing soil N 2 O emissions (Snyder et al., 2009). However, modifying one of the 4R components by itself may not be reliable in reducing N 2 O emissions, particularly in rainfed cropping systems (Decock, 2014). For example, the use of delayed application and/or split application (Sp) while maintaining N rate, source, and placement (Phillips et al., 2009;Zebarth et al., 2008Zebarth et al., , 2012 or the use of specialized N fertilizer sources (e.g., urea containing microbial inhibitors [UI]) while maintaining N rate, timing, and placement (Parkin and Hatfield, 2014;Sistani et al., 2011) have been inconsistent in reducing N 2 O emissions.The inconsistency of single-modification strategies is likely due to interactions of crop, soil, and weather factors. Recent studies in Minnesota corn systems using broadcast urea (U) showed no effectiveness of inhibitors alone over 5 site-years (Maharjan and Venterea, 2013;Venterea et al., 2011a) or timing alone over 2 site-years . Few studies have attempted to optimize combinations of timing, source, and rate to maintain corn yield and decrease N 2 O. Burzaco et al. (2013Burzaco et al. ( , 2014) meas...