Corn oil or corn grain supplementation to steers grazing endophyte-free tall fescue. II. Effects on subcutaneous fatty acid content and lipogenic gene expression1

Duckett, S. K.; Pratt, Scott L.; Paván, E.

doi:10.2527/jas.2008-1420

Cited by 84 publications

(72 citation statements)

References 48 publications

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“…Heifers assigned to MI had greater (P = 0.01) mRNA expression of FASN on day 51 compared with CON heifers (Table 6); an enzyme that modulates de novo lipogenesis and is up-regulated by rate of nutrient intake (Duckett et al, 2009). Moreover, MI heifers had greater mean mRNA expression of subcutaneous adipocyte PPARγ (P = 0.05), which regulates adipocyte development via triglyceride uptake (Rosen and MacDougald, 2006), and hepatic IGF-I (P < 0.01) compared with CON heifers (Table 6).…”

mentioning

confidence: 99%

Creep-feeding to stimulate metabolic imprinting in nursing beef heifers: impacts on heifer growth, reproductive and physiological variables

Cooke

Cappellozza

Marques

et al. 2015

Animal

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This experiment compared growth, physiological, and reproductive responses of beef heifers with (MI) or without (CON) access to a creep-feeder, as a manner to stimulate metabolic imprinting while nursing their dams. On day 0, 60 Angus × Hereford heifers were ranked by BW and age (140 ± 3 kg and 68 ± 3 days), and assigned to pairs so all ranking criteria were similar between heifers within each pair. On day 1, pairs were randomly assigned to MI (n = 15) or CON (n = 15). From day 1 to 51, MI pairs and their dams were allocated to 15 drylot pens where heifers had ad libitum access to a corn-based supplement through a creep-feeder. The CON pairs and their dams were maintained in an adjacent single drylot pen. From day 52 to 111, treatments were managed as a single group on a semiarid range pasture. On day 111, heifers were weaned and allocated to two pastures (one pasture/ treatment), receiving hay and a corn-based concentrate until day 326. Heifer BW was recorded before and at the end of the creepfeeding period (day 1 to 51), and on days 112 and 326. On days 0, 51, 111, 187, 261, and 325, jugular blood was collected and real-time ultrasonography for longissimus muscle depth and backfat thickness assessment was performed. Blood was also collected every 10 days from days 113 to 323 for puberty evaluation via plasma progesterone. Liver and subcutaneous fat biopsies were performed on days 51, 111, 261 and 325. Average daily gain was greater ( P < 0.01) for MI than CON from day 1 to 51, tended ( P = 0.09) to be greater for CON than MI from day 112 to 326, while BW on day 326 was similar between treatments. On day 51, MI had greater ( P ⩽ 0.01) plasma IGF-I and glucose concentrations, as well as mRNA expression of hepatic pyruvate carboxylase and adipose fatty acid synthase than CON. On days 261 and 325, plasma insulin concentrations were greater ( P ⩽ 0.03) in CON than MI. Mean mRNA expression of hepatic IGF-I and adipose peroxisome proliferator-activated receptor gamma were greater ( P ⩽ 0.05) in MI than CON. No treatment effects were detected for puberty attainment rate. In conclusion, supplementing nursing heifers via creep-feeding for 50 days altered physiological and biochemical variables suggestive of a metabolic imprinting effect, but did not hasten their puberty attainment.Keywords: growth, heifer, physiology, puberty, supplementation ImplicationsFeeding a high-concentrate supplement to nursing beef heifers for 50 days did not enhance their post-weaning body development, physiological responses, and puberty attainment. However, supplemented heifers had long-term increases in biochemical variables suggestive of metabolic imprinting, which is defined as biological responses to a nutritional intervention during early life that permanently alters physiological outcomes later in life. Perhaps a longer period of creep-feeding may be required to further increase supplement intake in nursing beef heifers, and effectively enhance body and reproductive development via metabolic imprinting effects. † E-mail: reinaldo.cooke...

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confidence: 99%

Creep-feeding to stimulate metabolic imprinting in nursing beef heifers: impacts on heifer growth, reproductive and physiological variables

Cooke

Cappellozza

Marques

et al. 2015

Animal

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“…The decreased n-3/n-6 ratio in grazing beef is a consequence of increased ALA proportion and a relative constant proportion of LA in adipose tissues from steers on pasture compared with those finished with feedlot diets. Steers finished on a grain diet show a 2.5 times higher n-6/n-3 PUFAs ratio than those finished on pasture (Basarab et al, 2007;Duckett et al, 2009). However, the fold of increase in the n-6/n-3 FA ratio can be higher when examined in beef muscles (Garcia et al, 2008).…”

Section: Discussionmentioning

confidence: 91%

Diet and Genotype Effects on n-3 Polyunsaturated Fatty Acids of Beef Lipids

García¹,

Latimori²,

Sancho³

et al. 2017

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“…4 The changes in these fatty acid composition of the subcutaneous adipose tissue suggest that de novo lipogenesis, desaturase, and elongase activities in the adipocytes were heightened with corn grain supplementation. Duckett et al 11 reported 46-fold and 18-fold upregulation of SCD-1 mRNA expression in subcutaneous adipose tissue in steers finishing on high-concentrate diets (85% corn grain) or corn grain supplementation (0.5% of body weight/d), respectively, compared with steers finished on pasture only. Exposure to corn grain during the finishing phase of beef production increases adipocyte hypertrophy and lipogenic enzyme activities to alter fatty acid composition.…”

Section: Discussionmentioning

confidence: 99%

“…10 Corn grain supplementation to grazing steers can also alter fat deposition, fatty acid composition and lipogenic gene expression of adipose tissue depots. 11,12 Fat deposition in finishing animals is the result of both hyperplasia and hypertrophy. [13][14][15] We hypothesized that the nutritional milieu of the donor animals from which SV cells were isolated from would alter proliferative, adipogenic, and lipogenic capacity of preadipocytes/adipocytes.…”

Section: Introductionmentioning

confidence: 99%

Nutritional milieu of isolated stromal vascular cells determines their proliferative, adipogenic, and lipogenic capacity in vitro

2014

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The objective was to determine the effect of nutritional milieu of isolated stromal vascular (SV) cells on proliferative capacity of preadipocytes, and adipogenic and lipogenic capacity in adipocytes in vitro. Proliferation of the preadipocytes increased over time with 48 and 72 h being greater than 24 h; however, preadipocytes from steers supplemented with corn (LC) had lower proliferation rates compared with those without corn grain supplementation (L) at 72 h. Adipocyte cultures isolated from LC group had higher mean diameter on d 4 and 6, and higher mean volume on d 0, 4, 6, and 12 of culture. Adipocytes from steers supplemented with corn grain (LC) had lower expression of key adipogenic genes during extended days in culture. The results show that prior nutritional treatment of the donor animal used to isolate SV cultures alters their proliferative, adipogenic, and lipogenic capacity in culture. These differences may be related to lower induction/expression of AP2 gene in the adipose cultures from corn supplemented group. Corn grain supplementation to steers grazing legumes could have stimulated more active adipogenic progenitor cells to differentiate, which would leave fewer behind in the SV pool for subsequent isolation.

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Corn oil or corn grain supplementation to steers grazing endophyte-free tall fescue. II. Effects on subcutaneous fatty acid content and lipogenic gene expression1

Cited by 84 publications

References 48 publications

Creep-feeding to stimulate metabolic imprinting in nursing beef heifers: impacts on heifer growth, reproductive and physiological variables

Creep-feeding to stimulate metabolic imprinting in nursing beef heifers: impacts on heifer growth, reproductive and physiological variables

Diet and Genotype Effects on n-3 Polyunsaturated Fatty Acids of Beef Lipids

Nutritional milieu of isolated stromal vascular cells determines their proliferative, adipogenic, and lipogenic capacity in vitro

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