Corn gluten hydrolyzates differentially regulate gene expression in epididymal fat by diet induced obese rats

Abstract

The aim of this study was to examine the effect of corn gluten hydrolyzates on signal transduction for body weight reduction in high fat‐induced obese rats. In the previous study, we confirmed that corn gluten hydrolyztes were effective for body fat reduction and reducing leptin and increasing adiponection level. Using this animal, we further analyzed mRNA and protein levels related to leptin and adiponectin signals. We investigated DNA manipulations through microarray and real time PCR. The corn peptide hydrolyztes feeding affected lipid metabolism gene expressions which are related low density lipoprotein receptor(LDLR), steroid 5‐alpha‐reductase 2, protein phosphatase methylesterase 1(PME‐1) and apolipoprotein B(apo B). The corn peptide hydrolyzates feeding decreased also mRNA level for leptin, while increasing adiponectin, fatty acid binding protein(FABP), insulin receptor(INSR), LDL receptor, peroxisome‐proliferator‐activated receptor‐gamma (PPAR‐γ) and mammalian target of rapamycin (mTOR). Our findings provide a molecular mechanism to explain a beneficial effect of corn gluten hydrolyzates on ameliorating diet induced obesity in mammals.