Corm induction and multiplication of<i>Amorphophallus albus in vitro</i>

Hu, Jianbin; Liu, J.; Xie, Conghua; Gao, Xiaoxia

doi:10.1080/14620316.2006.11512150

Cited by 4 publications

(6 citation statements)

References 14 publications

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“…Amorphophallus species are characterised by a single leaf and an underground stem (Figure 1A), and are important sources of konjac glucomannan (KGM) (Chua et al, 2010;Yin et al, 2019). Early investigations show that KGM is a multi-purpose material, with uses as a gelling agent, thickener, film former, and emulsifier (Nishinari et al, 1992;Hu et al, 2006). KGM has also been reported to have the ability to control blood cholesterol and sugar levels, help with weight loss and promote intestinal activity (Zhang et al, 2005;Hu et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide SNPs Provide Insights on the Cryptic Genetic Structure and Signatures of Climate Adaption in Amorphophallus albus Germplasms

et al. 2021

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Domesticated species represent unique systems in which the evolutionary genomic consequences of intensive selective breeding and adaptation can be thoroughly investigated. Amorphophallus albus occurs naturally and is in cultivation throughout the downstream region of the Jinshajiang River in Southwest China. This species is characterised by high konjac glucomannan content, and has been cultivated in China for nearly 2,000 years. To study genetic differentiation and local adaption of A. albus, we sampled 13 distinct local cultivated populations of this species. Restriction site-associated DNA sequencing was conducted with 87 samples, resulting in 24,225 SNPs. The population structure analyses suggest two main genetic groups: one in the relatively upstream region, and one downstream. We found evidence of additional sub-structure within the upstream group, demonstrating the statistical power of genomic SNPs in discovering subtle genetic structure. The environmental and geographic factors were all identified as significant in shaping the genetic differentiation of this species. Notably, the proportion of environmental factors was larger than geographic factors in influencing the population genetic patterns of A. albus. We also discovered loci that were associated with local adaptation. These findings will help us understand the genetic differentiation of this newly domesticated species, thereby informing future breeding programs of A. albus.

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Section: Introductionmentioning

confidence: 99%

Genome-Wide SNPs Provide Insights on the Cryptic Genetic Structure and Signatures of Climate Adaption in Amorphophallus albus Germplasms

et al. 2021

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“…Generally 1/2 to full concentration of salt were used and 30 gl -1 sucrose was added to culture medium for shoot proliferation (Faisal et al 2006). In a procedure for rooting of shoots, reduced concentration of medium salt and sucrose were used (Khan et al 1999;Singh et al 2008) However, in bulbous plants, high concentration of culture medium salt and sucrose was oftenly applied to produce more shoots and increase bulblet growth (Takayama and Misawa 1983;Bonnier and Van Tuyl 1997;Hu et al 2006;Joshi and Dhar 2009). In this study, shoot number and fresh weight per explant had increased significantly on medium containing double strength of LS salt and 50 gl -1 sucrose.…”

Section: Resultsmentioning

confidence: 99%

In vitro micropropagation of water hyacinth (Eichhornia crassipes)

Suh¹,

Park²,

Han³

2010

Journal of Plant Biotechnology

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This study was conducted to refine a micropropagation method of water hyacinth (Eichhornia crassipes) in vitro. When young shoots were cultured on media with various concentrations of BA or TDZ alone, LS medium containing 5.0 mgl-1 BA was found favorable for shoot proliferation from young shoots with a mean of 4.2 shoots. Using BA together with IAA, more shoots were obtained on LS medium containing 5.0 mgl-1 BA and 1.0 mgl-1 IAA with a mean of 5.7 shoots. In liquid medium, number of shoots and fresh weight per explant increased significantly. The best shoot proliferation and increasing of fresh weight were achieved on LS liquid medium containing 5.0 mgl-1 BA and 1.0 mgl-1 IAA with 6.9 shoots and more than 4,000 mg fresh weight. Of the different concentrations of LS salt, double strength of LS medium provided the highest shoot proliferation with 7.3 shoots, and fresh weight with 5,539 mg per explant. Shoot proliferation on LS medium containing 50 gl-1 sucrose had better results with 8.7 shoots and 5,979 mg per explant in fresh weight than other conditions. In conclusion, the optimal level for shoot proliferation and biomass increase of water hyacinth was attained with the application of the double strength of LS medium containing 5.0 mgl-1 BA, 1.0 mgl-1 IAA and 50 gl-1 sucrose.

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“…hortensis (syn A. paeoniifolius) secara in vitro telah dilakukan oleh (Irawati et al, 1986) dari eksplan yang berasal dari mata tunas umbi dan dapat menghasilkan bibit yang normal. Jenis-jenis Amorphophallus lainnya yang telah dipelajari cara perbanyakannya melalui teknik kultur jaringan diantaranya adalah A. albus (Hu et al, 2008;Hu et al, 2006), A. konjac (Zhao, 2012), A. muelleri (Imelda, 2008;Imelda et al, 2007;Prayana et al, 2017), A. oncophyllus (Amphawan et al, 2001), A. rivieri (Hu et al, 2005), A paeoniifolius (Kamala & Makeshkumar, 2014;Kayal et al, 2015) dan A. titanium (Irawati, 2011;Witjaksono et al, 2012).…”

Section: Pendahuluanunclassified

“…Data on the number of explants to form shoot, number of explants to form roots and the average number of roots were processed and transformed into (x + 1.5) 1/2 and data on number of explants to form callus were processed and transformed into (x + 0.5) 1/2. KK: Coefficient of Variation Secara umum hasil penelitian ini mirip dengan hasil terbaik untuk kultur tangkai daun A. albus pada media MS dengan penambahan 2 mg/l BA dan 0,5 mg/l NAA (Hu et al, 2006). Pada media tersebut, 71,2 % eksplan membentuk struktur menyerupai umbi (corm like structure) dan 26,7% dari struktur tersebut berdiferensiasi membentuk tunas.…”

Section: Hasil Dan Pembahasanunclassified

Regenerasi Tunas Suweg (Amorphophallus paeoniifolius (Dennst.) Nicolson) pada Berbagai Konsentrasi BAP dan NAA dengan Kondisi Penyimpanan Terang dan Gelap

Isnaini

Novitasari²

2020

Agriprima

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Amorphopallus paeoniifolius atau suweg merupakan salah satu jenis dari suku Araceae yang umbinya berpotensi untuk dikembangkan sebagai alternatif pangan karena memiliki kandungan gizi yang baik dan indeks glikemik yang rendah. Namun, perbanyakan suweg terkendala dalam ketersediaan bibitnya sehingga dibutuhkan alternatif perbanyakan, salah satunya dengan teknik kultur jaringan. Penelitian ini bertujuan untuk mendapatkan kombinasi media, jenis eksplan dan kondisi kultur yang optimal untuk perbanyakan suweg secara in vitro. Penelitian dilakukan dengan dua percobaan. Percobaan pertama yaitu regenerasi tunas dari eksplan tangkai daun yang ditanam pada media Murashige dan Skoog (MS) yang ditambahkan dengan BAP (0, 1, 2, dan 4 mg/l) dan NAA (0 dan 0,5 mg/l). Percobaan kedua yaitu regenerasi tunas dari eksplan tangkai daun dan kalus pada kondisi gelap dan terang. Hasil penelitian menunjukkan bahwa media MS dengan penambahan 2 mg/l BAP dan 0,5 mg/l NAA merupakan media yang paling baik bagi eksplan tangkai daun dalam pembentukan kalus, tunas dan akar yang ditandai dengan jumlah masing-masing adalah 75%, 50% dan 56,67%. Selanjutnya, eksplan berupa kalus lebih mudah memberikan respon membentuk tunas dan akar dibandingkan dengan tangkai daun, baik pada kondisi ruang penyimpanan gelap maupun terang.

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Corm induction and multiplication ofAmorphophallus albus in vitro

Cited by 4 publications

References 14 publications

Genome-Wide SNPs Provide Insights on the Cryptic Genetic Structure and Signatures of Climate Adaption in Amorphophallus albus Germplasms

Genome-Wide SNPs Provide Insights on the Cryptic Genetic Structure and Signatures of Climate Adaption in Amorphophallus albus Germplasms

In vitro micropropagation of water hyacinth (Eichhornia crassipes)

Regenerasi Tunas Suweg (Amorphophallus paeoniifolius (Dennst.) Nicolson) pada Berbagai Konsentrasi BAP dan NAA dengan Kondisi Penyimpanan Terang dan Gelap

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