Corilagin attenuates osteoclastic osteolysis by enhancing HO‐1 and inhibiting ROS

Abstract

Chinese herbal medicine has well‐established therapeutic effects in various diseases. Corilagin (Cor), a gallic acid tannin in Phyllanthus niruri L., has anti‐inflammatory and antioxidant effects in many diseases. However, its role in osteoclast‐related bone diseases has not been determined. In vitro, bone marrow macrophages (BMMs) were extracted and isolated to differentiate into osteoclasts. The effects of Cor on osteoclast formation, bone resorption, and reactive oxygen species (ROS) production were performed. In addition, quantitative real‐time polymerase chain reaction and western blot analysis were used to evaluate the effect of Cor on oxidative stress‐related pathways, which are nuclear factors‐κB ligand‐receptor activator (RANKL) stimulates important downstream pathways. Furthermore, microcomputed tomography and bone histomorphometry were performed to analyze the therapeutic effect of Cor in mouse models of lipopolysaccharide (LPS)‐mediated bone defects in vivo. Cor influenced the nuclear factor of activated T cells 1 (NFATc1) signaling pathway and reduced ROS in RANKL‐treated osteoclasts, thereby inhibiting osteoclast formation and bone resorption. Moreover, Cor protected against LPS‐mediated skull defects in vivo. In sum, our results confirm that Cor can inhibit osteoclastogenesis and intracellular oxidative stress. In addition, the inflammatory bone defect induced by LPS was also attenuated by Cor. Accordingly, Cor is a new candidate therapeutic agent for osteoclast‐mediated osteolytic diseases.

The bone marrow lipidomics of mice reveal sex‐related differences

“…Our results showed lower levels of C1P in bone marrow cells in female mice than male mice, suggesting that a lower level of C1P in female is detrimental to osteoblastogenesis. In our previous study, we found that the occurrence of OP is related to inflammation (Tan et al, 2022). Sphingolipid or Sph-like compounds have been shown to promote inflammasome activation and stimulate the release of the proinflammatory mediator IL-1β (Pyne et al, 2016).…”

Corilagin attenuates airway inflammation and collagen deposition in ovalbumin-induced asthmatic mice

“…Corilagin has been reported to have effects on several cardiovascular diseases (CVDs), including hypertension, atherosclerosis, stroke, congestive heart failure, and ischemic cardiomyopathy. 16 Moreover, it has antioxidant, anti-tumor, and anti-virus effects. 17,,18 Corilagin inhibited the activation of oligomerization domain-like receptor family pyrin domain-containing 3 (NLRP3) inflammasome and pyroptosis via the reactive oxygen species-thioredoxin-interacting protein-NLRP3 (ROS/TXNIP/NLRP3) axis to suppress inflammation.…”

Paradoxical role of reactive oxygen species in bone remodelling: implications in osteoporosis and possible nanotherapeutic interventions

“…Recently, clinical studies suggested that ROS-antioxidant systems may have a key role in the pathogenesis of bone loss [49]. ROS can easily activate the differentiation of osteoclasts from preosteoclasts which is followed by enhanced bone resorption [50,51] (Figure 1). Plethora of literature suggests that human bone marrow mononuclear cells when incubated with H 2 O 2 show enhanced osteoclasts count and their activity increases significantly along with tartrate resistant acid phosphatase (TRAP) levels [46], thereby favouring osteoclastogenesis.…”