Coriander Leaf Extract Exerts Antioxidant Activity and Protects Against UVB-Induced Photoaging of Skin by Regulation of Procollagen Type I and MMP-1 Expression

Abstract

Ultraviolet (UV) radiation causes photodamage to the skin, which, in turn, leads to depletion of the dermal extracellular matrix and chronic alterations in skin structure. Skin wrinkles are associated with collagen synthesis and matrix metalloproteinase-1 (MMP-1) activity. Coriandrum sativum L. (coriander leaf, cilantro; CS) has been used as a herbal medicine for the treatment of diabetes, hyperlipidemia, liver disease, and cancer. In this study, we examined whether CS ethanol extract (CSE) has protective effects against UVB-induced skin photoaging in normal human dermal fibroblasts (NHDF) in vitro and in the skin of hairless mice in vivo. The main component of CSE, linolenic acid, was determined by gas chromatography-mass spectroscopy. We measured the cellular levels of procollagen type I and MMP-1 using ELISA in NHDF cells after UVB irradiation. NHDF cells that were treated with CSE after UVB irradiation exhibited higher procollagen type I production and lower levels of MMP-1 than untreated cells. We found that the activity of transcription factor activator protein-1 (AP-1) was also inhibited by CSE treatment. We measured the epidermal thickness, dermal collagen fiber density, and procollagen type I and MMP-1 levels in photo-aged mouse skin in vivo using histological staining and western blot analysis. Our results showed that CSE-treated mice had thinner epidermal layers and denser dermal collagen fibers than untreated mice. On a molecular level, it was further confirmed that CSE-treated mice had lower MMP-1 levels and higher procollagen type I levels than untreated mice. Our results support the potential of C. sativum L. to prevent skin photoaging.

Antiphotoaging and Antimelanogenesis Properties of Ginsenoside C‐Y, a Ginsenoside Rb2 Metabolite from American Ginseng PDD‐ginsenoside

“…In photoaging skin, expression levels of type I procollagen and MMPs are regulated by the MAPK/AP‐1 signaling pathways . In addition, UVB exposure activates MMP‐1 overexpression, a pivotal enzyme in type I collagen degradation, which accelerates the rate of skin senility . In our study, UVB‐induced phosphorylation of AP‐1 and MAPK was prominently suppressed by treatment with ginsenoside C‐Y to NHDF cells.…”

A review on chemical constituents and pharmacological activities of Coriandrum sativum

“…CSE-treated mice had thinner epidermal layers and denser dermal collagen fibers than untreated mice. On a molecular level, it was further confirmed that CSE-treated mice had lower MMP-1 levels and higher procollagen type I levels than untreated mice [169].…”

Antiaging effects of the mixture of Panax ginseng and Crataegus pinnatifida in human dermal fibroblasts and healthy human skin

“…The mechanisms of the skin that lead to photoaging are induced mainly by the repetitive absorption of UV radiation, which can generate reactive oxygen species and stimulate the expression of extracellular matrix proteases via AP-1 signaling [29]. AP-1, made of Jun and Fos family proteins, is a transcription factor that inhibits collagen production and upregulates collagen breakdown by activating enzymes called MMPs [30].…”

“…Because the skin's natural antioxidant defenses are disrupted by high doses of UV light, reactive oxygen species are considered to be key factors involved in the initiation of the skin aging process [23]. Our previous studies demonstrated that Aloe vera , coriander, azuki beans, and Galla chinensis , all of which have antioxidant activity, have protective effects against UVB-induced skin aging [23], [29], [31], [32]. There have been many studies elucidating the antioxidant activities of P. ginseng and C. pinnatifida .…”